Kinetic Computational Alanine Scanning: Application to p53 Oligomerization. Export

@article{citeulike:505496, abstract = {We have developed a novel computational alanine scanning approach that involves analysis of ensemble unfolding kinetics at high temperature to identify residues that are critical for the stability of a given protein. This approach has been applied to dimerization of the oligomerization domain (residues 326-355) of tumor suppressor p53. As validated by experimental results, our approach has reasonable success in identifying deleterious mutations, including mutations that have been linked to cancer. We discuss a method for determining the effect of mutations on the location of the dimerization transition state.}, address = {Departments of Chemistry, Structural Biology, Stanford University, Stanford, CA 94305-5447, USA; IBM Almaden Research Center, 650 Harry Road, San Jose, CA 95120, USA.}, author = {Chong, Lillian T T. and Swope, William C C. and Pitera, Jed W W. and Pande, Vijay S S.}, citeulike-article-id = {505496}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jmb.2005.12.083}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16457841}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16457841}, day = {17}, doi = {10.1016/j.jmb.2005.12.083}, issn = {0022-2836}, journal = {J Mol Biol}, keywords = {computational, oligomerization, p53}, month = {January}, posted-at = {2006-02-15 03:31:44}, priority = {2}, title = {Kinetic Computational Alanine Scanning: Application to p53 Oligomerization.}, url = {http://dx.doi.org/10.1016/j.jmb.2005.12.083}, year = {2006} }

TY - JOUR ID - citeulike:505496 L3 - citeulike-article-id:505496 N2 - We have developed a novel computational alanine scanning approach that involves analysis of ensemble unfolding kinetics at high temperature to identify residues that are critical for the stability of a given protein. This approach has been applied to dimerization of the oligomerization domain (residues 326-355) of tumor suppressor p53. As validated by experimental results, our approach has reasonable success in identifying deleterious mutations, including mutations that have been linked to cancer. We discuss a method for determining the effect of mutations on the location of the dimerization transition state. JF - J Mol Biol SN - 0022-2836 AD - Departments of Chemistry, Structural Biology, Stanford University, Stanford, CA 94305-5447, USA; IBM Almaden Research Center, 650 Harry Road, San Jose, CA 95120, USA. TI - Kinetic Computational Alanine Scanning: Application to p53 Oligomerization. KW - computational KW - oligomerization KW - p53 AU - Chong, Lillian T AU - Swope, William C AU - Pitera, Jed W AU - Pande, Vijay S PY - 2006/01/17/ UR - http://dx.doi.org/10.1016/j.jmb.2005.12.083 ER -