Group: Khavkin's lab - with tag molecular [4 articles]

Abstract

In just the past 20 years systematics has progressed from the sequencing of individual genes for a few taxa to routine sequencing of complete plastid and even nuclear genomes. Recent technological advances have made it possible to compile very large data sets, the analyses of which have in turn provided unprecedented insights into phylogeny and evolution. Indeed, this narrow window of a few decades will likely be viewed as a golden era in systematics. Relationships have been resolved at all taxonomic ...

Abstract

Although molecular markers allow more accurate selection in early generations than conventional screens, large numbers can make selection impracticable while screening in later generations may provide little or no advantage over conventional selection techniques. Investigation of different crossing strategies and consideration of when to screen, what proportion to retain and the impacts of dominant vs. codominant marker expression revealed important choices in the design of marker-assisted selection programs that can produce large efficiency gains. Using F2 enrichment increased the frequency of ...