Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella

@article{citeulike:2786387, abstract = {A peptide nucleic acid (PNA) probe was developed to detect the toxic dinoflagellate, Takayama pulchella TPXM, using fluorescent in situ hybridization (FISH) combined with epifluorescent microscopy and flow cytometry. The PNA probe was then used to analyze HAB samples from Xiamen Bay. The results indicated that the fluorescein phosphoramidite (FAM)-labeled probe (PNATP28S01) [Flu]-OO ATG CCA TCT CAA GA, entered the algal cells easily and bound to the target species specifically. High hybridization efficiency (nearly 100\%) was observed. Detection by epifluorescence microscopy and flow cytometry gave comparable results. The fluorescence intensity of the PNA probe hybridized to T. pulchella cells was remarkably higher than that of two DNA probes used in this study and than the autofluorescence of the blank and negative control cells. In addition, the hybridization condition of the PNA probe was easier to control than DNA probes, and when applied to field-collected samples, the PNA probe showed higher binding efficiency to the target species than DNA probes. With the observed high specificity, binding efficiency, and detection signal intensity, the PNA probe will be useful for monitoring harmful algal blooms of T. pulchella.}, author = {Huang, Bangqin and Hou, Jianjun and Lin, Senjie and Chen, Jixin and Hong, Huasheng }, citeulike-article-id = {2786387}, doi = {10.1016/j.hal.2007.11.006}, journal = {Harmful Algae}, keywords = {18s, dinoflagellate, phytoplankton, probes, toxic}, month = {June}, number = {4}, pages = {495--503}, posted-at = {2008-05-12 05:43:11}, priority = {2}, title = {Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella}, url = {http://dx.doi.org/10.1016/j.hal.2007.11.006}, volume = {7}, year = {2008} }

TY - JOUR ID - citeulike:2786387 L3 - citeulike-article-id:2786387 TI - Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella JF - Harmful Algae VL - 7 IS - 4 SP - 495 EP - 503 N2 - A peptide nucleic acid (PNA) probe was developed to detect the toxic dinoflagellate, Takayama pulchella TPXM, using fluorescent in situ hybridization (FISH) combined with epifluorescent microscopy and flow cytometry. The PNA probe was then used to analyze HAB samples from Xiamen Bay. The results indicated that the fluorescein phosphoramidite (FAM)-labeled probe (PNATP28S01) [Flu]-OO ATG CCA TCT CAA GA, entered the algal cells easily and bound to the target species specifically. High hybridization efficiency (nearly 100%) was observed. Detection by epifluorescence microscopy and flow cytometry gave comparable results. The fluorescence intensity of the PNA probe hybridized to T. pulchella cells was remarkably higher than that of two DNA probes used in this study and than the autofluorescence of the blank and negative control cells. In addition, the hybridization condition of the PNA probe was easier to control than DNA probes, and when applied to field-collected samples, the PNA probe showed higher binding efficiency to the target species than DNA probes. With the observed high specificity, binding efficiency, and detection signal intensity, the PNA probe will be useful for monitoring harmful algal blooms of T. pulchella. KW - 18s KW - dinoflagellate KW - phytoplankton KW - probes KW - toxic AU - Huang, Bangqin AU - Hou, Jianjun AU - Lin, Senjie AU - Chen, Jixin AU - Hong, Huasheng PY - 2008/06// UR - http://dx.doi.org/10.1016/j.hal.2007.11.006 ER -