Acute colitis induction by oil of mustard results in later development of an IBS-like accelerated upper GI transit in mice Export

@article{citeulike:2747145, abstract = {Oil of mustard (OM) is a potent neuronal activator that promotes allodynia and hyperalgesia within minutes of application. In this study, OM was used to induce an acute colitis. We also investigated whether intracolonic OM-induced inflammation alters gastrointestinal (GI) function over a longer time frame as a model of postinflammatory irritable bowel syndrome (PI-IBS). Mice given a single administration of 0.5\% OM developed a severe colitis that peaked at day 3, was reduced at day 7, and was absent by day 14. At the peak response, there was body weight loss, colon shrinkage, thickening and weight increases, distension of the proximal colon, and diarrhea. Macroscopic inspection of the distal colon revealed a discontinuous pattern of inflammatory damage and occasional transmural ulceration. Histological examination showed loss of epithelium, an inflammatory infiltrate, destruction of mucosal architecture, edema, and loss of circular smooth muscle architecture. OM administration increased transit of a carmine dye bolus from 58\% of the total length of the upper GI tract in untreated age-matched controls to as high as 74\% when tested at day 28 post-OM. Mice in the latter group demonstrated a significantly more sensitive response to inhibition of upper GI transit by the {micro}-opioid receptor agonist loperamide compared with normal mice. OM induces a rapid, acute, and transient colitis and, in the longer term, functional changes in motility that are observed when there is no gross inflammation and thereby is a model of functional bowel disorders that mimic aspects of PI-IBS in humans. 10.1152/ajpgi.00444.2004}, author = {Kimball, Edward S. and Palmer, Jeffrey M. and D'Andrea, Michael R. and Hornby, Pamela J. and Wade, Paul R.}, citeulike-article-id = {2747145}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpgi.00444.2004}, citeulike-linkout-1 = {http://ajpgi.physiology.org/cgi/content/abstract/288/6/G1266}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15691868}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15691868}, doi = {10.1152/ajpgi.00444.2004}, journal = {Am J Physiol Gastrointest Liver Physiol}, keywords = {bowel, disease, inflammatory, intestine, irritable, large, postinflammatory, small, syndrome}, month = {June}, number = {6}, pages = {G1266--1273}, posted-at = {2008-05-02 22:59:59}, priority = {3}, title = {Acute colitis induction by oil of mustard results in later development of an IBS-like accelerated upper GI transit in mice}, url = {http://dx.doi.org/10.1152/ajpgi.00444.2004}, volume = {288}, year = {2005} }

TY - JOUR ID - citeulike:2747145 L3 - citeulike-article-id:2747145 N2 - Oil of mustard (OM) is a potent neuronal activator that promotes allodynia and hyperalgesia within minutes of application. In this study, OM was used to induce an acute colitis. We also investigated whether intracolonic OM-induced inflammation alters gastrointestinal (GI) function over a longer time frame as a model of postinflammatory irritable bowel syndrome (PI-IBS). Mice given a single administration of 0.5% OM developed a severe colitis that peaked at day 3, was reduced at day 7, and was absent by day 14. At the peak response, there was body weight loss, colon shrinkage, thickening and weight increases, distension of the proximal colon, and diarrhea. Macroscopic inspection of the distal colon revealed a discontinuous pattern of inflammatory damage and occasional transmural ulceration. Histological examination showed loss of epithelium, an inflammatory infiltrate, destruction of mucosal architecture, edema, and loss of circular smooth muscle architecture. OM administration increased transit of a carmine dye bolus from 58% of the total length of the upper GI tract in untreated age-matched controls to as high as 74% when tested at day 28 post-OM. Mice in the latter group demonstrated a significantly more sensitive response to inhibition of upper GI transit by the {micro}-opioid receptor agonist loperamide compared with normal mice. OM induces a rapid, acute, and transient colitis and, in the longer term, functional changes in motility that are observed when there is no gross inflammation and thereby is a model of functional bowel disorders that mimic aspects of PI-IBS in humans. 10.1152/ajpgi.00444.2004 IS - 6 JF - Am J Physiol Gastrointest Liver Physiol EP - 1273 TI - Acute colitis induction by oil of mustard results in later development of an IBS-like accelerated upper GI transit in mice VL - 288 SP - G1266 KW - bowel KW - disease KW - inflammatory KW - intestine KW - irritable KW - large KW - postinflammatory KW - small KW - syndrome AU - Kimball, Edward AU - Palmer, Jeffrey AU - D'Andrea, Michael AU - Hornby, Pamela AU - Wade, Paul PY - 2005/06/01/ UR - http://dx.doi.org/10.1152/ajpgi.00444.2004 ER -