Effects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes Export

@article{citeulike:3210682, abstract = {Glucosamine induced insulin resistance in 3T3-L1 adipocytes, which was associated with a 15\% decrease in cellular ATP content. To study the role of ATP depletion in insulin resistance, we employed sodium azide (NaN3) and dinitrophenol (DNP), which affect mitochondrial oxidative phosphorylation, to achieve a similar 15\% ATP depletion. Unlike glucosamine, NaN3 and DNP markedly increased basal glucose transport, and the increased basal glucose transport was associated with increased GLUT-1 content in the plasma membrane without changes in total GLUT-1 content. These agents, like glucosamine, did not affect the early insulin signaling that is implicated in insulin stimulation of glucose transport. In cells with a severe 40\% ATP depletion, basal glucose transport was similarly elevated, and insulin-stimulated glucose transport was similar in cells with 15\% ATP depletion. In these cells, however, early insulin signaling was severely diminished. These data suggest that cellular ATP depletion by glucosamine, NaN3, and DNP exerts differential effects on basal and insulin-stimulated glucose transport and that ATP depletion per se does not induce insulin resistance in 3T3-L1 adipocytes.}, author = {Kang, Jione and Heart, Emma and Sung, Chin K.}, citeulike-article-id = {3210682}, citeulike-linkout-0 = {http://ajpendo.physiology.org/cgi/content/abstract/280/3/E428}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11171597}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11171597}, day = {1}, journal = {Am J Physiol Endocrinol Metab}, keywords = {adipocytes, atp-depletion, glucose\_transport, insulin\_resistance}, month = {March}, number = {3}, pages = {E428--435}, posted-at = {2008-09-09 19:13:09}, priority = {3}, title = {Effects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes}, url = {http://ajpendo.physiology.org/cgi/content/abstract/280/3/E428}, volume = {280}, year = {2001} }

TY - JOUR ID - citeulike:3210682 L3 - citeulike-article-id:3210682 N2 - Glucosamine induced insulin resistance in 3T3-L1 adipocytes, which was associated with a 15% decrease in cellular ATP content. To study the role of ATP depletion in insulin resistance, we employed sodium azide (NaN3) and dinitrophenol (DNP), which affect mitochondrial oxidative phosphorylation, to achieve a similar 15% ATP depletion. Unlike glucosamine, NaN3 and DNP markedly increased basal glucose transport, and the increased basal glucose transport was associated with increased GLUT-1 content in the plasma membrane without changes in total GLUT-1 content. These agents, like glucosamine, did not affect the early insulin signaling that is implicated in insulin stimulation of glucose transport. In cells with a severe 40% ATP depletion, basal glucose transport was similarly elevated, and insulin-stimulated glucose transport was similar in cells with 15% ATP depletion. In these cells, however, early insulin signaling was severely diminished. These data suggest that cellular ATP depletion by glucosamine, NaN3, and DNP exerts differential effects on basal and insulin-stimulated glucose transport and that ATP depletion per se does not induce insulin resistance in 3T3-L1 adipocytes. IS - 3 JF - Am J Physiol Endocrinol Metab EP - 435 TI - Effects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes VL - 280 SP - E428 KW - adipocytes KW - atp-depletion KW - glucose_transport KW - insulin_resistance AU - Kang, Jione AU - Heart, Emma AU - Sung, Chin PY - 2001/03/01/ UR - http://ajpendo.physiology.org/cgi/content/abstract/280/3/E428 ER -