Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells. Export

@article{citeulike:4405656, abstract = {OBJECTIVE AND DESIGN: We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells. METHODS: The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry. RESULTS: Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells. CONCLUSION: These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.}, author = {Matsushima, Miyoko and Takagi, Kenzo and Ogawa, Miyuki and Hirose, Etsuko and Ota, Yui and Abe, Fumie and Baba, Kenji and Hasegawa, Takaaki and Hasegawa, Yoshinori and Kawabe, Tsutomu}, citeulike-article-id = {4405656}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00011-009-0039-1}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19390785}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19390785}, citeulike-linkout-3 = {http://www.springerlink.com/content/54t001498v137678}, doi = {10.1007/s00011-009-0039-1}, issn = {1420-908X}, journal = {Inflammation research : official journal of the European Histamine Research Society ... [et al.]}, keywords = {ho-1, nrf2}, month = {October}, number = {10}, pages = {705--715}, posted-at = {2009-09-22 23:38:08}, priority = {2}, title = {Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells.}, url = {http://dx.doi.org/10.1007/s00011-009-0039-1}, volume = {58}, year = {2009} }

TY - JOUR ID - citeulike:4405656 L3 - citeulike-article-id:4405656 N2 - OBJECTIVE AND DESIGN: We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells. METHODS: The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry. RESULTS: Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells. CONCLUSION: These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway. IS - 10 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] SN - 1420-908X EP - 715 TI - Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells. VL - 58 SP - 705 KW - ho-1 KW - nrf2 AU - Matsushima, Miyoko AU - Takagi, Kenzo AU - Ogawa, Miyuki AU - Hirose, Etsuko AU - Ota, Yui AU - Abe, Fumie AU - Baba, Kenji AU - Hasegawa, Takaaki AU - Hasegawa, Yoshinori AU - Kawabe, Tsutomu PY - 2009/10// UR - http://dx.doi.org/10.1007/s00011-009-0039-1 ER -