C-reactive protein inhibits adiponectin gene expression and secretion in 3T3-L1 adipocytes Export

@article{citeulike:4698578, abstract = {C-reactive protein (CRP) is considered as one of the most sensitive markers of inflammation. The aim of the present study is to investigate the effects of CRP on the production of adiponectin in 3T3-L1 adipocytes. Northern and western blot analysis revealed that CRP treatment inhibited adiponectin mRNA expression and secretion in a dose- and time-dependent manner. Co-incubation of adipocytes with rosiglitazone and CRP decreased induction of adiponectin gene expression by rosiglitazone. However, luciferase reporter assays did not show that CRP affected the activity of \~{}2.1 kb adiponectin gene promoter, which was increased by rosiglitazone alone. Pharmacological inhibition of phosphatidylinositol (PI)-3 kinase by LY294002 partially reversed inhibition of adiponectin gene expression by CRP. These results collectively suggest that CRP suppresses adiponectin gene expression partially through the PI-3 kinase pathway, and that decreased production of adiponectin might represent a mechanism by which CRP regulates insulin sensitivity. 10.1677/JOE-07-0133}, author = {Yuan, Guoyue and Chen, Xia and Ma, Qinyun and Qiao, Jie and Li, Rongying and Li, Xuesong and Li, Shengxian and Tang, Jinfeng and Zhou, Libin and Song, Huaidong and Chen, Mingdao}, citeulike-article-id = {4698578}, citeulike-linkout-0 = {http://dx.doi.org/10.1677/JOE-07-0133}, citeulike-linkout-1 = {http://joe.endocrinology-journals.org/cgi/content/abstract/194/2/275}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17641277}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17641277}, doi = {10.1677/JOE-07-0133}, journal = {J Endocrinol}, keywords = {adipocytes, adiponectin, c-reactive\_protein}, month = {August}, number = {2}, pages = {275--281}, posted-at = {2009-06-01 11:52:28}, priority = {3}, title = {C-reactive protein inhibits adiponectin gene expression and secretion in 3T3-L1 adipocytes}, url = {http://dx.doi.org/10.1677/JOE-07-0133}, volume = {194}, year = {2007} }

TY - JOUR ID - citeulike:4698578 L3 - citeulike-article-id:4698578 N2 - C-reactive protein (CRP) is considered as one of the most sensitive markers of inflammation. The aim of the present study is to investigate the effects of CRP on the production of adiponectin in 3T3-L1 adipocytes. Northern and western blot analysis revealed that CRP treatment inhibited adiponectin mRNA expression and secretion in a dose- and time-dependent manner. Co-incubation of adipocytes with rosiglitazone and CRP decreased induction of adiponectin gene expression by rosiglitazone. However, luciferase reporter assays did not show that CRP affected the activity of ~2.1 kb adiponectin gene promoter, which was increased by rosiglitazone alone. Pharmacological inhibition of phosphatidylinositol (PI)-3 kinase by LY294002 partially reversed inhibition of adiponectin gene expression by CRP. These results collectively suggest that CRP suppresses adiponectin gene expression partially through the PI-3 kinase pathway, and that decreased production of adiponectin might represent a mechanism by which CRP regulates insulin sensitivity. 10.1677/JOE-07-0133 IS - 2 JF - J Endocrinol EP - 281 TI - C-reactive protein inhibits adiponectin gene expression and secretion in 3T3-L1 adipocytes VL - 194 SP - 275 KW - adipocytes KW - adiponectin KW - c-reactive_protein AU - Yuan, Guoyue AU - Chen, Xia AU - Ma, Qinyun AU - Qiao, Jie AU - Li, Rongying AU - Li, Xuesong AU - Li, Shengxian AU - Tang, Jinfeng AU - Zhou, Libin AU - Song, Huaidong AU - Chen, Mingdao PY - 2007/08/01/ UR - http://dx.doi.org/10.1677/JOE-07-0133 ER -