Ductal carcinoma in situ and the emergence of diversity during breast cancer evolution. Export

@article{citeulike:3214228, abstract = {PURPOSE: Human invasive breast cancers (IBC) show enormous histologic and biological diversity. This study comprehensively evaluated diversity in ductal carcinoma in situ (DCIS), the immediate precursors of IBCs. EXPERIMENTAL DESIGN: The extent of diversity for conventional histologic grade and standard prognostic biomarkers assessed by immunohistochemistry was evaluated in a series of pure DCIS (n = 200) compared with a contemporaneous series of IBCs (n = 200). A subset of the DCIS (n = 25) was evaluated by DNA microarrays for the presence of luminal, basal, and erbB2 intrinsic subtypes. The extent of diversity within individual cases of DCIS (n = 120) was determined by assessing multiple regions independently for histologic (nuclear) grade and several biomarkers by immunohistochemistry, which approximate microarrays in determining intrinsic subtypes. RESULTS: DCIS showed a broad distribution of conventional histologic grades and standard biomarkers ranging from well to poorly differentiated, nearly identical to IBCs. Microarrays showed the same intrinsic subtypes in DCIS as in IBCs. However, higher resolution analysis showed that multiple histologic grades, biomarker phenotypes, and intrinsic subtypes often coexist within the same DCIS, and these diverse regions probably compete for dominance. Diversity within cases of DCIS was highly correlated with mutated p53 (P = 0.0007). CONCLUSIONS: These results support the hypothesis that poorly differentiated DCIS gradually evolve from well-differentiated DCIS by randomly acquiring genetic defects resulting in increasingly abnormal cellular features. This diversity is amplified by defects resulting in genetic instability (e.g., p53 mutation), and the alterations are propagated to IBC in a manner independent of progression to invasion.}, address = {Department of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA. dcallred@path.wustl.edu}, author = {Allred, D. C. and Wu, Y. and Mao, S. and Nagtegaal, I. D. and Lee, S. and Perou, C. M. and Mohsin, S. K. and O'Connell, P. and Tsimelzon, A. and Medina, D.}, citeulike-article-id = {3214228}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/1078-0432.CCR-07-1127}, citeulike-linkout-1 = {http://clincancerres.aacrjournals.org/cgi/content/abstract/14/2/370}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18223211}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18223211}, day = {15}, doi = {10.1158/1078-0432.CCR-07-1127}, issn = {1078-0432}, journal = {Clinical cancer research : an official journal of the American Association for Cancer Research}, keywords = {breast, cancer, dcis, diversity, evolution, progression}, month = {January}, number = {2}, pages = {370--378}, posted-at = {2008-09-11 15:17:21}, priority = {2}, title = {Ductal carcinoma in situ and the emergence of diversity during breast cancer evolution.}, url = {http://dx.doi.org/10.1158/1078-0432.CCR-07-1127}, volume = {14}, year = {2008} }

TY - JOUR ID - citeulike:3214228 L3 - citeulike-article-id:3214228 N2 - PURPOSE: Human invasive breast cancers (IBC) show enormous histologic and biological diversity. This study comprehensively evaluated diversity in ductal carcinoma in situ (DCIS), the immediate precursors of IBCs. EXPERIMENTAL DESIGN: The extent of diversity for conventional histologic grade and standard prognostic biomarkers assessed by immunohistochemistry was evaluated in a series of pure DCIS (n = 200) compared with a contemporaneous series of IBCs (n = 200). A subset of the DCIS (n = 25) was evaluated by DNA microarrays for the presence of luminal, basal, and erbB2 intrinsic subtypes. The extent of diversity within individual cases of DCIS (n = 120) was determined by assessing multiple regions independently for histologic (nuclear) grade and several biomarkers by immunohistochemistry, which approximate microarrays in determining intrinsic subtypes. RESULTS: DCIS showed a broad distribution of conventional histologic grades and standard biomarkers ranging from well to poorly differentiated, nearly identical to IBCs. Microarrays showed the same intrinsic subtypes in DCIS as in IBCs. However, higher resolution analysis showed that multiple histologic grades, biomarker phenotypes, and intrinsic subtypes often coexist within the same DCIS, and these diverse regions probably compete for dominance. Diversity within cases of DCIS was highly correlated with mutated p53 (P = 0.0007). CONCLUSIONS: These results support the hypothesis that poorly differentiated DCIS gradually evolve from well-differentiated DCIS by randomly acquiring genetic defects resulting in increasingly abnormal cellular features. This diversity is amplified by defects resulting in genetic instability (e.g., p53 mutation), and the alterations are propagated to IBC in a manner independent of progression to invasion. IS - 2 JF - Clinical cancer research : an official journal of the American Association for Cancer Research SN - 1078-0432 AD - Department of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA. dcallred@path.wustl.edu EP - 378 TI - Ductal carcinoma in situ and the emergence of diversity during breast cancer evolution. VL - 14 SP - 370 KW - breast KW - cancer KW - dcis KW - diversity KW - evolution KW - progression AU - Allred, DC AU - Wu, Y AU - Mao, S AU - Nagtegaal, ID AU - Lee, S AU - Perou, CM AU - Mohsin, SK AU - O'Connell, P AU - Tsimelzon, A AU - Medina, D PY - 2008/01/15/ UR - http://dx.doi.org/10.1158/1078-0432.CCR-07-1127 ER -