Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells. Export

@article{citeulike:3423081, abstract = {It has been proposed that cytokines play a role in the pathogenesis of chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS). However, different studies have reported conflicting results using enzyme-linked immunosorbent assay or polymerase chain reaction to detect cytokines in these conditions. In the present study, for the first time, the production of inflammatory [interleukin (IL)-1alpha, IL-6, and TNF-alpha] and anti-inflammatory (IL-10) cytokines by CD14+ and CD14- peripheral blood mononuclear cells (PBMC) from chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS) patients and sex- and age-matched normal subjects was investigated at the level of individual cells using the technique of intracellular cytokine staining and flow cytometry. Cultures were carried out in the presence of polymyxin B to inhibit the effect of endotoxins on cytokine production by monocytes. The mean intensity of fluorescence (MIF) and percentage of CD14+ (monocytes) and CD14- (lymphocytes) cytokine-producing mononuclear cells were comparable in patients and controls in either unstimulated or IFN-gamma-stimulated conditions. Our study indicates that dysregulation of cytokine production by circulating monocytes or non-monocytic cells (lymphocytes) is not a dominant factor in the pathogenesis of CFS/FMS.}, author = {Amel Kashipaz, M. R. and Swinden, D. and Todd, I. and Powell, R. J.}, citeulike-article-id = {3423081}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12699429}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12699429}, issn = {0009-9104}, journal = {Clinical and experimental immunology}, keywords = {cfs, chroni\_fatigue\_syndrome, cytokines, fibromyalgia}, month = {May}, number = {2}, pages = {360--365}, posted-at = {2008-10-17 04:34:07}, priority = {3}, title = {Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12699429}, volume = {132}, year = {2003} }

TY - JOUR ID - citeulike:3423081 L3 - citeulike-article-id:3423081 N2 - It has been proposed that cytokines play a role in the pathogenesis of chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS). However, different studies have reported conflicting results using enzyme-linked immunosorbent assay or polymerase chain reaction to detect cytokines in these conditions. In the present study, for the first time, the production of inflammatory [interleukin (IL)-1alpha, IL-6, and TNF-alpha] and anti-inflammatory (IL-10) cytokines by CD14+ and CD14- peripheral blood mononuclear cells (PBMC) from chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS) patients and sex- and age-matched normal subjects was investigated at the level of individual cells using the technique of intracellular cytokine staining and flow cytometry. Cultures were carried out in the presence of polymyxin B to inhibit the effect of endotoxins on cytokine production by monocytes. The mean intensity of fluorescence (MIF) and percentage of CD14+ (monocytes) and CD14- (lymphocytes) cytokine-producing mononuclear cells were comparable in patients and controls in either unstimulated or IFN-gamma-stimulated conditions. Our study indicates that dysregulation of cytokine production by circulating monocytes or non-monocytic cells (lymphocytes) is not a dominant factor in the pathogenesis of CFS/FMS. IS - 2 JF - Clinical and experimental immunology SN - 0009-9104 EP - 365 TI - Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells. VL - 132 SP - 360 KW - cfs KW - chroni_fatigue_syndrome KW - cytokines KW - fibromyalgia AU - Amel Kashipaz, MR AU - Swinden, D AU - Todd, I AU - Powell, RJ PY - 2003/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/12699429 ER -