Accurate determination of microbial diversity from 454 pyrosequencing data Export

by: Christopher Quince, Anders Lanzen, Thomas P. Curtis, Russell J. Davenport, Neil Hall, Ian M. Head, L. Fiona Read, William T. Sloan

Nature Methods, Vol. 6, No. 9. (09 August 2009), pp. 639-641.

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Abstract

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

@article{citeulike:5415594, abstract = {We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.}, author = {Quince, Christopher and Lanzen, Anders and Curtis, Thomas P. and Davenport, Russell J. and Hall, Neil and Head, Ian M. and Read, L. Fiona and Sloan, William T.}, citeulike-article-id = {5415594}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nmeth.1361}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nmeth.1361}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19668203}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19668203}, day = {09}, doi = {10.1038/nmeth.1361}, issn = {1548-7091}, journal = {Nature Methods}, keywords = {454, diversity, otu}, month = {August}, number = {9}, pages = {639--641}, posted-at = {2009-08-21 22:29:55}, priority = {2}, publisher = {Nature Publishing Group}, title = {Accurate determination of microbial diversity from 454 pyrosequencing data}, url = {http://dx.doi.org/10.1038/nmeth.1361}, volume = {6}, year = {2009} }

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TY - JOUR ID - citeulike:5415594 L3 - citeulike-article-id:5415594 N2 - We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated. IS - 9 JF - Nature Methods SN - 1548-7091 EP - 641 TI - Accurate determination of microbial diversity from 454 pyrosequencing data PB - Nature Publishing Group VL - 6 SP - 639 KW - 454 KW - diversity KW - otu AU - Quince, Christopher AU - Lanzen, Anders AU - Curtis, Thomas AU - Davenport, Russell AU - Hall, Neil AU - Head, Ian AU - Read, Fiona AU - Sloan, William PY - 2009/08/09/ UR - http://dx.doi.org/10.1038/nmeth.1361 ER -

Accurate determination of microbial diversity from 454 pyrosequencing data Export

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