Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome". Export

@article{citeulike:735694, abstract = {The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single genome does not reflect how genetic variability drives pathogenesis within a bacterial species and also limits genome-wide screens for vaccine candidates or for antimicrobial targets. We have generated the genomic sequence of six strains representing the five major disease-causing serotypes of Streptococcus agalactiae, the main cause of neonatal infection in humans. Analysis of these genomes and those available in databases showed that the S. agalactiae species can be described by a pan-genome consisting of a core genome shared by all isolates, accounting for approximately 80\% of any single genome, plus a dispensable genome consisting of partially shared and strain-specific genes. Mathematical extrapolation of the data suggests that the gene reservoir available for inclusion in the S. agalactiae pan-genome is vast and that unique genes will continue to be identified even after sequencing hundreds of genomes.}, address = {Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.}, author = {Tettelin, Herv\'{e} and Masignani, Vega and Cieslewicz, Michael J. and Donati, Claudio and Medini, Duccio and Ward, Naomi L. and Angiuoli, Samuel V. and Crabtree, Jonathan and Jones, Amanda L. and Durkin, A. Scott and Deboy, Robert T. and Davidsen, Tanja M. and Mora, Marirosa and Scarselli, Maria and Margarit y Ros, Immaculada and Peterson, Jeremy D. and Hauser, Christopher R. and Sundaram, Jaideep P. and Nelson, William C. and Madupu, Ramana and Brinkac, Lauren M. and Dodson, Robert J. and Rosovitz, Mary J. and Sullivan, Steven A. and Daugherty, Sean C. and Haft, Daniel H. and Selengut, Jeremy and Gwinn, Michelle L. and Zhou, Liwei and Zafar, Nikhat and Khouri, Hoda and Radune, Diana and Dimitrov, George and Watkins, Kisha and O'Connor, Kevin J. and Smith, Shannon and Utterback, Teresa R. and White, Owen and Rubens, Craig E. and Grandi, Guido and Madoff, Lawrence C. and Kasper, Dennis L. and Telford, John L. and Wessels, Michael R. and Rappuoli, Rino and Fraser, Claire M.}, citeulike-article-id = {735694}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0506758102}, citeulike-linkout-1 = {http://www.pnas.org/content/102/39/13950.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/102/39/13950.full.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/16172379}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=16172379}, day = {27}, doi = {10.1073/pnas.0506758102}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {comparative, core, genome, genomics, hgt, pangenome, population, prokaryotes}, month = {September}, number = {39}, pages = {13950--13955}, posted-at = {2009-07-14 20:00:07}, priority = {0}, title = {Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome".}, url = {http://dx.doi.org/10.1073/pnas.0506758102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:735694 L3 - citeulike-article-id:735694 N2 - The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single genome does not reflect how genetic variability drives pathogenesis within a bacterial species and also limits genome-wide screens for vaccine candidates or for antimicrobial targets. We have generated the genomic sequence of six strains representing the five major disease-causing serotypes of Streptococcus agalactiae, the main cause of neonatal infection in humans. Analysis of these genomes and those available in databases showed that the S. agalactiae species can be described by a pan-genome consisting of a core genome shared by all isolates, accounting for approximately 80% of any single genome, plus a dispensable genome consisting of partially shared and strain-specific genes. Mathematical extrapolation of the data suggests that the gene reservoir available for inclusion in the S. agalactiae pan-genome is vast and that unique genes will continue to be identified even after sequencing hundreds of genomes. IS - 39 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. EP - 13955 TI - Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome". VL - 102 SP - 13950 KW - comparative KW - core KW - genome KW - genomics KW - hgt KW - pangenome KW - population KW - prokaryotes AU - Tettelin, Hervé AU - Masignani, Vega AU - Cieslewicz, Michael AU - Donati, Claudio AU - Medini, Duccio AU - Ward, Naomi AU - Angiuoli, Samuel AU - Crabtree, Jonathan AU - Jones, Amanda AU - Durkin, Scott AU - Deboy, Robert AU - Davidsen, Tanja AU - Mora, Marirosa AU - Scarselli, Maria AU - Margarit y Ros, Immaculada AU - Peterson, Jeremy AU - Hauser, Christopher AU - Sundaram, Jaideep AU - Nelson, William AU - Madupu, Ramana AU - Brinkac, Lauren AU - Dodson, Robert AU - Rosovitz, Mary AU - Sullivan, Steven AU - Daugherty, Sean AU - Haft, Daniel AU - Selengut, Jeremy AU - Gwinn, Michelle AU - Zhou, Liwei AU - Zafar, Nikhat AU - Khouri, Hoda AU - Radune, Diana AU - Dimitrov, George AU - Watkins, Kisha AU - O'Connor, Kevin AU - Smith, Shannon AU - Utterback, Teresa AU - White, Owen AU - Rubens, Craig AU - Grandi, Guido AU - Madoff, Lawrence AU - Kasper, Dennis AU - Telford, John AU - Wessels, Michael AU - Rappuoli, Rino AU - Fraser, Claire PY - 2005/09/27/ UR - http://dx.doi.org/10.1073/pnas.0506758102 ER -