Resveratrol Inhibits Transcription of CYP1A1 in Vitro by Preventing Activation of the Aryl Hydrocarbon Receptor Export

@article{citeulike:3978990, abstract = {Resveratrol, a compound present in a variety of plants, was recently shown to have potent chemopreventive activity against aryl hydrocarbon-induced tumorigenesis in mice. Therefore, in the present study, we examined the effect of resveratrol on the function of the aryl hydrocarbon receptor (AHR) and the transcription of CYP1A1 in human HepG2 hepatoma cells. Resveratrol inhibited the increase in cytochrome P450 (CYP) 1A1 mRNA caused by the AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a concentration-dependent manner. The induction of transcription of an aryl hydrocarbon-responsive reporter vector containing the CYP1A1 promoter by TCDD was likewise inhibited by resveratrol. Resveratrol also inhibited the constitutive level of CYP1A1 mRNA and reporter vector transcription in HepG2 cells. The increase in CYP1A1 enzyme activity induced by TCDD was inhibited by resveratrol. Resveratrol prevented the TCDD-induced transformation of the cytosolic AHR to its nuclear DNA-binding form. However, resveratrol had no effect on the binding of TCDD to the cytosolic AHR. These data demonstrate that resveratrol inhibits CYP1A1 expression in vitro, and that it does this by preventing the binding of the AHR to promoter sequences that regulate CYP1A1 transcription. This activity may be an important part of the chemopreventive activity of resveratrol.}, author = {Ciolino, Henry P. and Daschner, Phillip J. and Yeh, Grace C.}, citeulike-article-id = {3978990}, citeulike-linkout-0 = {http://cancerres.aacrjournals.org/cgi/content/abstract/58/24/5707}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9865727}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9865727}, day = {15}, journal = {Cancer Res}, keywords = {ahr, cyp1a1, resveratrol}, month = {December}, number = {24}, pages = {5707--5712}, posted-at = {2009-01-29 13:53:54}, priority = {3}, title = {Resveratrol Inhibits Transcription of CYP1A1 in Vitro by Preventing Activation of the Aryl Hydrocarbon Receptor}, url = {http://cancerres.aacrjournals.org/cgi/content/abstract/58/24/5707}, volume = {58}, year = {1998} }

TY - JOUR ID - citeulike:3978990 L3 - citeulike-article-id:3978990 N2 - Resveratrol, a compound present in a variety of plants, was recently shown to have potent chemopreventive activity against aryl hydrocarbon-induced tumorigenesis in mice. Therefore, in the present study, we examined the effect of resveratrol on the function of the aryl hydrocarbon receptor (AHR) and the transcription of CYP1A1 in human HepG2 hepatoma cells. Resveratrol inhibited the increase in cytochrome P450 (CYP) 1A1 mRNA caused by the AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a concentration-dependent manner. The induction of transcription of an aryl hydrocarbon-responsive reporter vector containing the CYP1A1 promoter by TCDD was likewise inhibited by resveratrol. Resveratrol also inhibited the constitutive level of CYP1A1 mRNA and reporter vector transcription in HepG2 cells. The increase in CYP1A1 enzyme activity induced by TCDD was inhibited by resveratrol. Resveratrol prevented the TCDD-induced transformation of the cytosolic AHR to its nuclear DNA-binding form. However, resveratrol had no effect on the binding of TCDD to the cytosolic AHR. These data demonstrate that resveratrol inhibits CYP1A1 expression in vitro, and that it does this by preventing the binding of the AHR to promoter sequences that regulate CYP1A1 transcription. This activity may be an important part of the chemopreventive activity of resveratrol. IS - 24 JF - Cancer Res EP - 5712 TI - Resveratrol Inhibits Transcription of CYP1A1 in Vitro by Preventing Activation of the Aryl Hydrocarbon Receptor VL - 58 SP - 5707 KW - ahr KW - cyp1a1 KW - resveratrol AU - Ciolino, Henry AU - Daschner, Phillip AU - Yeh, Grace PY - 1998/12/15/ UR - http://cancerres.aacrjournals.org/cgi/content/abstract/58/24/5707 ER -