Whey protein potentiates the intestinotrophic action of glucagon-like peptide-2 in parenterally fed rats Export

@article{citeulike:6051482, abstract = {Glucagon-like peptide-2 (GLP-2) is a nutrient-regulated intestinotrophic hormone derived from proglucagon in the distal intestine. Enteral nutrients (EN) potentiate the action of GLP-2 to reverse parenteral nutrition (PN)-induced mucosal hypoplasia. The objective was to determine what enteral protein component, casein, soy, or whey protein, potentiates the intestinal growth response to GLP-2 in rats with PN-induced mucosal hypoplasia. Rats received PN and continuous intravenous infusion of GLP-2 (100 {micro}g/kg/day) for 7 days. Six EN groups received PN+GLP-2 for days 1-3 and partial PN+GLP-2 plus EN for days 4-7. EN was provided by ad libitum intake of a semielemental liquid diet with different protein sources: casein, hydrolyzed soy, whey protein concentrate (WPC), and hydrolyzed WPC+casein. Controls received PN+GLP-2 alone. EN induced significantly greater jejunal sucrase activity and gain of body weight, and improved feed efficiency compared with PN+GLP-2 alone. EN induced greater ileal proglucagon expression, increased plasma concentration of bioactive GLP-2 by 35\%, and reduced plasma dipeptidyl peptidase IV (DPP-IV) activity compared with PN+GLP-2 alone, P < 0.05. However, only whey protein, and not casein or soy, potentiated the ability of GLP-2 to reverse PN-induced mucosal hypoplasia and further increase ileal villus height, crypt depth, and mucosa cellularity compared with PN+GLP-2 alone, P < 0.05. The ability of whey protein to induce greater mucosal surface area was associated with decreased DPP-IV activity in ileum and colon compared with casein, soy, or PN+GLP-2 alone, P < 0.05. In conclusion, whey protein potentiates the action of GLP-2 to reverse PN-induced mucosal hypoplasia in association with decreased intestinal DPP-IV activity. 10.1152/ajpregu.00423.2009}, author = {Liu, Xiaowen and Murali, Sangita G. and Holst, Jens J. and Ney, Denise M.}, citeulike-article-id = {6051482}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpregu.00423.2009}, citeulike-linkout-1 = {http://ajpregu.physiology.org/content/297/5/R1554.abstract}, citeulike-linkout-2 = {http://ajpregu.physiology.org/content/297/5/R1554.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19776251}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19776251}, day = {1}, doi = {10.1152/ajpregu.00423.2009}, journal = {Am J Physiol Regul Integr Comp Physiol}, month = {November}, number = {5}, pages = {R1554--1562}, posted-at = {2009-10-31 20:33:09}, priority = {3}, title = {Whey protein potentiates the intestinotrophic action of glucagon-like peptide-2 in parenterally fed rats}, url = {http://dx.doi.org/10.1152/ajpregu.00423.2009}, volume = {297}, year = {2009} }

TY - JOUR ID - citeulike:6051482 L3 - citeulike-article-id:6051482 N2 - Glucagon-like peptide-2 (GLP-2) is a nutrient-regulated intestinotrophic hormone derived from proglucagon in the distal intestine. Enteral nutrients (EN) potentiate the action of GLP-2 to reverse parenteral nutrition (PN)-induced mucosal hypoplasia. The objective was to determine what enteral protein component, casein, soy, or whey protein, potentiates the intestinal growth response to GLP-2 in rats with PN-induced mucosal hypoplasia. Rats received PN and continuous intravenous infusion of GLP-2 (100 {micro}g/kg/day) for 7 days. Six EN groups received PN+GLP-2 for days 1-3 and partial PN+GLP-2 plus EN for days 4-7. EN was provided by ad libitum intake of a semielemental liquid diet with different protein sources: casein, hydrolyzed soy, whey protein concentrate (WPC), and hydrolyzed WPC+casein. Controls received PN+GLP-2 alone. EN induced significantly greater jejunal sucrase activity and gain of body weight, and improved feed efficiency compared with PN+GLP-2 alone. EN induced greater ileal proglucagon expression, increased plasma concentration of bioactive GLP-2 by 35%, and reduced plasma dipeptidyl peptidase IV (DPP-IV) activity compared with PN+GLP-2 alone, P < 0.05. However, only whey protein, and not casein or soy, potentiated the ability of GLP-2 to reverse PN-induced mucosal hypoplasia and further increase ileal villus height, crypt depth, and mucosa cellularity compared with PN+GLP-2 alone, P < 0.05. The ability of whey protein to induce greater mucosal surface area was associated with decreased DPP-IV activity in ileum and colon compared with casein, soy, or PN+GLP-2 alone, P < 0.05. In conclusion, whey protein potentiates the action of GLP-2 to reverse PN-induced mucosal hypoplasia in association with decreased intestinal DPP-IV activity. 10.1152/ajpregu.00423.2009 IS - 5 JF - Am J Physiol Regul Integr Comp Physiol EP - 1562 TI - Whey protein potentiates the intestinotrophic action of glucagon-like peptide-2 in parenterally fed rats VL - 297 SP - R1554 AU - Liu, Xiaowen AU - Murali, Sangita AU - Holst, Jens AU - Ney, Denise PY - 2009/11/01/ UR - http://dx.doi.org/10.1152/ajpregu.00423.2009 ER -