Analysis of the multiple roles of gld-1 in germline development: interactions with the sex-determination cascade and the glp-1 signaling pathway. Export

@article{citeulike:3276828, abstract = {The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathway is set in the female mode (terminal fem/fog genes inactive), gld-1 (null) germ cells exit meiotic prophase and proliferate to form a tumor, but when the pathway is set in the male mode, they develop into sperm. We conclude that the gld-1 (null) phenotype is cell-type specific and that gld-1(+) acts at the end of the cascade to direct oogenesis. We also use cell ablation and epistasis analysis to examine the dependence of tumor formation on the glp-1 signalling pathway. Although glp-1 activity promotes tumor growth, it is not essential for tumor formation by gld-1 (null) germ cells. These data also reveal that gld-1(+) plays a nonessential (and sex nonspecific) role in regulating germ cell proliferation before their entry into meiosis. Thus gld-1(+) may negatively regulate proliferation at two distinct points in germ cell development: before entry into meiotic prophase in both sexes (nonessential premeiotic gld-1 function) and during meiotic prophase when the sex determination pathway is set in the female mode (essential meiotic gld-1 function).}, author = {Francis, R. and Maine, E. M. and Schedl, T. B.}, citeulike-article-id = {3276828}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7713420}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7713420}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00002114}, journal = {Genetics}, keywords = {article, c\_elegans, caenorhabditis\_elegans, celegans, cell\_division, elegans, epistasis\_genetic, f02a96, genes\_helminth\_physiology, genes\_suppressor\_tumor\_physiology, germ\_cells\_cytology, germ\_cells\_physiology, germinoma, gld-1, glp-1, helminth\_proteins\_physiology, hermaphroditism, meiosis, membrane\_glycoproteins\_physiology, mutation\_genetics, nematode, oogenesis\_genetics, phenotype, prophase, sex\_differentiation\_genetics, signal\_transduction, spermatogenesis\_genetics, t23g113, wormbase}, pages = {607--630}, posted-at = {2008-09-17 00:02:38}, priority = {3}, title = {Analysis of the multiple roles of gld-1 in germline development: interactions with the sex-determination cascade and the glp-1 signaling pathway.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7713420}, volume = {139}, year = {1995} }

TY - JOUR ID - citeulike:3276828 L3 - citeulike-article-id:3276828 N2 - The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathway is set in the female mode (terminal fem/fog genes inactive), gld-1 (null) germ cells exit meiotic prophase and proliferate to form a tumor, but when the pathway is set in the male mode, they develop into sperm. We conclude that the gld-1 (null) phenotype is cell-type specific and that gld-1(+) acts at the end of the cascade to direct oogenesis. We also use cell ablation and epistasis analysis to examine the dependence of tumor formation on the glp-1 signalling pathway. Although glp-1 activity promotes tumor growth, it is not essential for tumor formation by gld-1 (null) germ cells. These data also reveal that gld-1(+) plays a nonessential (and sex nonspecific) role in regulating germ cell proliferation before their entry into meiosis. Thus gld-1(+) may negatively regulate proliferation at two distinct points in germ cell development: before entry into meiotic prophase in both sexes (nonessential premeiotic gld-1 function) and during meiotic prophase when the sex determination pathway is set in the female mode (essential meiotic gld-1 function). JF - Genetics EP - 630 TI - Analysis of the multiple roles of gld-1 in germline development: interactions with the sex-determination cascade and the glp-1 signaling pathway. VL - 139 SP - 607 KW - article KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - cell_division KW - elegans KW - epistasis_genetic KW - f02a96 KW - genes_helminth_physiology KW - genes_suppressor_tumor_physiology KW - germ_cells_cytology KW - germ_cells_physiology KW - germinoma KW - gld-1 KW - glp-1 KW - helminth_proteins_physiology KW - hermaphroditism KW - meiosis KW - membrane_glycoproteins_physiology KW - mutation_genetics KW - nematode KW - oogenesis_genetics KW - phenotype KW - prophase KW - sex_differentiation_genetics KW - signal_transduction KW - spermatogenesis_genetics KW - t23g113 KW - wormbase AU - Francis, R AU - Maine, EM AU - Schedl, TB PY - 1995/// UR - http://view.ncbi.nlm.nih.gov/pubmed/7713420 ER -