Extracellular proteins needed for C. elegans mechanosensation. Export

@article{citeulike:3278342, abstract = {The mec-5 and mec-9 genes encode putative extracellular proteins that allow a set of six touch receptor neurons in C. elegans to respond to gentle touch. MEC-5 is a collagen made by the epidermal cells that surround the touch cells. Mutations causing touch insensitivity affect the Gly-X-Y repeats of this collagen. mec-9 produces two transcripts, the larger of which is expressed in the touch cells and two PVD neurons. This transcript encodes a protein with 5 Kunitz-type protease inhibitor domains, 6 EGF-like repeats (2 of the Ca2+-binding type), and a glutamic acid-rich region. Missense mutations causing touch insensitivity affect both the EGF-like and Kunitz domains. Since mec-9 loss of function mutations dominantly enhance the touch insensitive phenotype of several mec-5 mutations, MEC-5 and MEC-9 may interact. We propose that these proteins provide an extracellular attachment point for the mechanosensory channels of the touch cells.}, author = {Du H-, P. and Gu, G. Q. and William, C. M. and Chalfie, M.}, citeulike-article-id = {3278342}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8562083}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8562083}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00002379}, journal = {Neuron}, keywords = {amino\_acid\_sequence, article, base\_sequence, c50h23, c\_elegans, caenorhabditis\_elegans, caenorhabditis\_elegans\_genetics, caenorhabditis\_elegans\_physiology, celegans, collagen\_genetics, collagen\_physiology, e03g23, elegans, epistasis\_genetic, extracellular\_matrix\_physiology, extracellular\_matrix\_proteins\_chemistry, extracellular\_matrix\_proteins\_genetics, extracellular\_matrix\_proteins\_physiology, f01d46, fluorescent\_dyes, genes\_recessive, genes\_reporter, genes\_structural\_helminth, helminth\_proteins\_chemistry, helminth\_proteins\_genetics, helminth\_proteins\_physiology, mec-3, mec-5, mec-9, mechanoreceptors\_physiology, molecular\_sequence\_data, mutagenesis\_site\_directed, nematode, neurons\_afferent\_chemistry, neurons\_afferent\_physiology, protein\_structure\_tertiary, recombinant\_fusion\_proteins\_analysis, repetitive\_sequences\_nucleic\_acid, sequence\_alignment, sequence\_homology\_amino\_acid, touch\_physiology, wormbase}, pages = {183--194}, posted-at = {2008-09-17 01:15:37}, priority = {3}, title = {Extracellular proteins needed for C. elegans mechanosensation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8562083}, volume = {16}, year = {1996} }

TY - JOUR ID - citeulike:3278342 L3 - citeulike-article-id:3278342 N2 - The mec-5 and mec-9 genes encode putative extracellular proteins that allow a set of six touch receptor neurons in C. elegans to respond to gentle touch. MEC-5 is a collagen made by the epidermal cells that surround the touch cells. Mutations causing touch insensitivity affect the Gly-X-Y repeats of this collagen. mec-9 produces two transcripts, the larger of which is expressed in the touch cells and two PVD neurons. This transcript encodes a protein with 5 Kunitz-type protease inhibitor domains, 6 EGF-like repeats (2 of the Ca2+-binding type), and a glutamic acid-rich region. Missense mutations causing touch insensitivity affect both the EGF-like and Kunitz domains. Since mec-9 loss of function mutations dominantly enhance the touch insensitive phenotype of several mec-5 mutations, MEC-5 and MEC-9 may interact. We propose that these proteins provide an extracellular attachment point for the mechanosensory channels of the touch cells. JF - Neuron EP - 194 TI - Extracellular proteins needed for C. elegans mechanosensation. VL - 16 SP - 183 KW - amino_acid_sequence KW - article KW - base_sequence KW - c50h23 KW - c_elegans KW - caenorhabditis_elegans KW - caenorhabditis_elegans_genetics KW - caenorhabditis_elegans_physiology KW - celegans KW - collagen_genetics KW - collagen_physiology KW - e03g23 KW - elegans KW - epistasis_genetic KW - extracellular_matrix_physiology KW - extracellular_matrix_proteins_chemistry KW - extracellular_matrix_proteins_genetics KW - extracellular_matrix_proteins_physiology KW - f01d46 KW - fluorescent_dyes KW - genes_recessive KW - genes_reporter KW - genes_structural_helminth KW - helminth_proteins_chemistry KW - helminth_proteins_genetics KW - helminth_proteins_physiology KW - mec-3 KW - mec-5 KW - mec-9 KW - mechanoreceptors_physiology KW - molecular_sequence_data KW - mutagenesis_site_directed KW - nematode KW - neurons_afferent_chemistry KW - neurons_afferent_physiology KW - protein_structure_tertiary KW - recombinant_fusion_proteins_analysis KW - repetitive_sequences_nucleic_acid KW - sequence_alignment KW - sequence_homology_amino_acid KW - touch_physiology KW - wormbase AU - Du H-, P AU - Gu, GQ AU - William, CM AU - Chalfie, M PY - 1996/// UR - http://view.ncbi.nlm.nih.gov/pubmed/8562083 ER -