Control of neuronal morphogenesis by homeoproteins: consequences for the making of neuronal networks. Export

@article{citeulike:3290727, abstract = {To test whether homeoproteins can act as genetic regulators in the processes of neurite growth, branching, guidance, and connectivity, the 60 amino acid homeodomain of Antennapedia was introduced in embryonic neurons in primary culture. It was hoped that this homeopeptide would bind to specific promoters and thus behave as a competitive inhibitor of endogenous homeoproteins. The introduction of the homeodomain in the nerve cells was made easy by its unexpected capability to translocate through the membranes and to accumulate within the nuclei. The presence of the homeodomain within the cells correlated with an increase in neurite growth and branching. The absence of activity of mutant peptides, still internalized but unable to bind with high affinity to homeoprotein cognate binding sites, strongly suggested that endogenous homeoproteins modulate neurite outgrowth and branching. Moreover, the efficient internalization of the homeobox peptide by live cells in culture raises the possibility that, in addition to their well-established role as cell-autonomous transcription factors, some homeoproteins may also exert paracrine functions. We examine how these hypotheses could modify our current views on the establishment and plasticity of neuronal networks.}, author = {Volovitch, M. and Le and Joliot, A. H. and Bloch and Prochiantz, A.}, citeulike-article-id = {3290727}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012176}, journal = {Perspectives in Developmental Neurobiology}, keywords = {article, c38d46, c\_elegans, caenorhabditis\_elegans, caenorhabditis\_elegans\_embryology, caenorhabditis\_elegans\_genetics, celegans, cell\_nucleus\_metabolism, dna\_binding\_proteins\_genetics, dna\_binding\_proteins\_physiology, drosophila\_melanogaster\_embryology, drosophila\_melanogaster\_genetics, elegans, f01d46, genes\_homeo\_box, lin-11, mec-3, mice\_embryology, mice\_genetics, models\_biological, morphogenesis\_genetics, motor\_neurons\_cytology, nematode, nerve\_net\_embryology, neurites\_ultrastructure, neurons\_cytology, pal-1, transcription\_factors\_genetics, transcription\_factors\_physiology, transfection, wormbase, zc2473}, pages = {133--138}, posted-at = {2008-09-18 22:02:28}, priority = {3}, title = {Control of neuronal morphogenesis by homeoproteins: consequences for the making of neuronal networks.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012176}, volume = {1}, year = {1993} }

TY - JOUR ID - citeulike:3290727 L3 - citeulike-article-id:3290727 N2 - To test whether homeoproteins can act as genetic regulators in the processes of neurite growth, branching, guidance, and connectivity, the 60 amino acid homeodomain of Antennapedia was introduced in embryonic neurons in primary culture. It was hoped that this homeopeptide would bind to specific promoters and thus behave as a competitive inhibitor of endogenous homeoproteins. The introduction of the homeodomain in the nerve cells was made easy by its unexpected capability to translocate through the membranes and to accumulate within the nuclei. The presence of the homeodomain within the cells correlated with an increase in neurite growth and branching. The absence of activity of mutant peptides, still internalized but unable to bind with high affinity to homeoprotein cognate binding sites, strongly suggested that endogenous homeoproteins modulate neurite outgrowth and branching. Moreover, the efficient internalization of the homeobox peptide by live cells in culture raises the possibility that, in addition to their well-established role as cell-autonomous transcription factors, some homeoproteins may also exert paracrine functions. We examine how these hypotheses could modify our current views on the establishment and plasticity of neuronal networks. JF - Perspectives in Developmental Neurobiology EP - 138 TI - Control of neuronal morphogenesis by homeoproteins: consequences for the making of neuronal networks. VL - 1 SP - 133 KW - article KW - c38d46 KW - c_elegans KW - caenorhabditis_elegans KW - caenorhabditis_elegans_embryology KW - caenorhabditis_elegans_genetics KW - celegans KW - cell_nucleus_metabolism KW - dna_binding_proteins_genetics KW - dna_binding_proteins_physiology KW - drosophila_melanogaster_embryology KW - drosophila_melanogaster_genetics KW - elegans KW - f01d46 KW - genes_homeo_box KW - lin-11 KW - mec-3 KW - mice_embryology KW - mice_genetics KW - models_biological KW - morphogenesis_genetics KW - motor_neurons_cytology KW - nematode KW - nerve_net_embryology KW - neurites_ultrastructure KW - neurons_cytology KW - pal-1 KW - transcription_factors_genetics KW - transcription_factors_physiology KW - transfection KW - wormbase KW - zc2473 AU - Volovitch, M AU - Le AU - Joliot, AH AU - Bloch AU - Prochiantz, A PY - 1993/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012176 ER -