Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme. Export

@article{citeulike:3290747, abstract = {By subtraction cloning we previously identified a set of mouse genes (named Nedd1 through Nedd10) with developmentally down-regulated expression in brain. We now show that one such gene, Nedd2, encodes a protein similar to the mammalian interleukin-1 beta-converting enzyme (ICE) and the product of the Caenorhabditis elegans cell death gene ced-3 (CED-3). Both ICE and CED-3 are known to encode putative cysteine proteases and induce apoptosis when overexpressed in cultured cells. Overexpression of Nedd2 in cultured fibroblast and neuroblastoma cells also resulted in cell death by apoptosis, which was suppressed by the expression of the human bcl-2 gene, indicating that Nedd2 is functionally similar to the ced-3 gene in C. elegans. We also show that during embryonic development, Nedd2 is highly expressed in several types of mouse tissue undergoing high rates of programmed cell death such as central nervous system and kidney. Our data suggest that Nedd2 is an important component of the mammalian programmed cell death machinery.}, author = {Kumar, S. and Kinoshita, M. and Noda, M. and Copeland, N. G. and Jenkins, N. A.}, citeulike-article-id = {3290747}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012199}, journal = {Genes and Development}, keywords = {amino\_acid\_sequence, article, base\_sequence, brain\_embryology, brain\_metabolism, c48d12, c\_elegans, caenorhabditis\_elegans, caenorhabditis\_elegans\_cytology, caenorhabditis\_elegans\_embryology, caenorhabditis\_elegans\_genetics, ced-3, celegans, cell\_death\_genetics, cells\_cultured, chromosome\_mapping, cloning\_molecular, cysteine\_proteinases\_biosynthesis, cysteine\_proteinases\_genetics, elegans, embryo, fetal\_development, genetic\_vectors, helminth\_proteins\_genetics, in\_situ\_hybridization, mammals\_genetics, mice\_genetics, molecular\_sequence\_data, nematode, organ\_specificity, sequence\_homology\_amino\_acid, transfection, wormbase}, pages = {1613--1626}, posted-at = {2008-09-18 22:06:57}, priority = {3}, title = {Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012199}, volume = {8}, year = {1994} }

TY - JOUR ID - citeulike:3290747 L3 - citeulike-article-id:3290747 N2 - By subtraction cloning we previously identified a set of mouse genes (named Nedd1 through Nedd10) with developmentally down-regulated expression in brain. We now show that one such gene, Nedd2, encodes a protein similar to the mammalian interleukin-1 beta-converting enzyme (ICE) and the product of the Caenorhabditis elegans cell death gene ced-3 (CED-3). Both ICE and CED-3 are known to encode putative cysteine proteases and induce apoptosis when overexpressed in cultured cells. Overexpression of Nedd2 in cultured fibroblast and neuroblastoma cells also resulted in cell death by apoptosis, which was suppressed by the expression of the human bcl-2 gene, indicating that Nedd2 is functionally similar to the ced-3 gene in C. elegans. We also show that during embryonic development, Nedd2 is highly expressed in several types of mouse tissue undergoing high rates of programmed cell death such as central nervous system and kidney. Our data suggest that Nedd2 is an important component of the mammalian programmed cell death machinery. JF - Genes and Development EP - 1626 TI - Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme. VL - 8 SP - 1613 KW - amino_acid_sequence KW - article KW - base_sequence KW - brain_embryology KW - brain_metabolism KW - c48d12 KW - c_elegans KW - caenorhabditis_elegans KW - caenorhabditis_elegans_cytology KW - caenorhabditis_elegans_embryology KW - caenorhabditis_elegans_genetics KW - ced-3 KW - celegans KW - cell_death_genetics KW - cells_cultured KW - chromosome_mapping KW - cloning_molecular KW - cysteine_proteinases_biosynthesis KW - cysteine_proteinases_genetics KW - elegans KW - embryo KW - fetal_development KW - genetic_vectors KW - helminth_proteins_genetics KW - in_situ_hybridization KW - mammals_genetics KW - mice_genetics KW - molecular_sequence_data KW - nematode KW - organ_specificity KW - sequence_homology_amino_acid KW - transfection KW - wormbase AU - Kumar, S AU - Kinoshita, M AU - Noda, M AU - Copeland, NG AU - Jenkins, NA PY - 1994/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012199 ER -