Most mRNAs in the nematode Ascaris lumbricoides are trans-spliced: a role for spliced leader addition in translational efficiency. Export

@article{citeulike:3290771, abstract = {Some pre-mRNAs in nematodes are processed by trans-splicing. In this reaction, a 22-nt 5' terminal exon (the spliced leader, SL) and its associated 2,2,7-trimethylguanosine cap are acquired from a specialized Sm snRNP, the SL RNP. Although it has been evident for many years that not all nematode mRNAs contain the SL sequence, the prevalence of trans-spliced mRNAs has, with the exception of Caenorhabditis elegans, not been determined. To address this question in an organism amenable to biochemical analysis, we have prepared a message-dependent protein synthesis system from developing embryos of the parasitic nematode, Ascaris lumbricoides. Using this system, we have used both hybrid-arrest and hybrid-selection approaches to show that the vast majority (80-90\%) of A. lumbricoides mRNAs contain the SL sequence and therefore are processed by trans-splicing. Furthermore, to examine the effect of SL addition on translation, we have measured levels of protein synthesis in extracts programmed with a variety of s ynthetic mRNAs. We find that the SL sequence itself and its associated hypermethylated cap functionally collaborate to enhance translational efficiency, presumably at the level of initiation of protein synthesis. These results indicate that trans-splicing plays a larger role in nematode gene expression than previously suspected.}, author = {Maroney, P. A. and Denker, J. A. and Darzynkiewicz, E. and Laneve, R. and Nilsen, T. W.}, citeulike-article-id = {3290771}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012232}, journal = {RNA}, keywords = {article, ascaris\_lumbricoides\_embryology, ascaris\_lumbricoides\_genetics, c\_elegans, caenorhabditis\_elegans, celegans, cell\_free\_system, elegans, genes\_helminth, nematode, nucleic\_acid\_hybridization, rna\_caps, rna\_helminth\_genetics, rna\_helminth\_metabolism, rna\_messenger\_genetics, rna\_messenger\_metabolism, rna\_splicing, translation\_genetic, wormbase}, pages = {714--723}, posted-at = {2008-09-18 22:12:08}, priority = {3}, title = {Most mRNAs in the nematode Ascaris lumbricoides are trans-spliced: a role for spliced leader addition in translational efficiency.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012232}, volume = {1}, year = {1995} }

TY - JOUR ID - citeulike:3290771 L3 - citeulike-article-id:3290771 N2 - Some pre-mRNAs in nematodes are processed by trans-splicing. In this reaction, a 22-nt 5' terminal exon (the spliced leader, SL) and its associated 2,2,7-trimethylguanosine cap are acquired from a specialized Sm snRNP, the SL RNP. Although it has been evident for many years that not all nematode mRNAs contain the SL sequence, the prevalence of trans-spliced mRNAs has, with the exception of Caenorhabditis elegans, not been determined. To address this question in an organism amenable to biochemical analysis, we have prepared a message-dependent protein synthesis system from developing embryos of the parasitic nematode, Ascaris lumbricoides. Using this system, we have used both hybrid-arrest and hybrid-selection approaches to show that the vast majority (80-90%) of A. lumbricoides mRNAs contain the SL sequence and therefore are processed by trans-splicing. Furthermore, to examine the effect of SL addition on translation, we have measured levels of protein synthesis in extracts programmed with a variety of s ynthetic mRNAs. We find that the SL sequence itself and its associated hypermethylated cap functionally collaborate to enhance translational efficiency, presumably at the level of initiation of protein synthesis. These results indicate that trans-splicing plays a larger role in nematode gene expression than previously suspected. JF - RNA EP - 723 TI - Most mRNAs in the nematode Ascaris lumbricoides are trans-spliced: a role for spliced leader addition in translational efficiency. VL - 1 SP - 714 KW - article KW - ascaris_lumbricoides_embryology KW - ascaris_lumbricoides_genetics KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - cell_free_system KW - elegans KW - genes_helminth KW - nematode KW - nucleic_acid_hybridization KW - rna_caps KW - rna_helminth_genetics KW - rna_helminth_metabolism KW - rna_messenger_genetics KW - rna_messenger_metabolism KW - rna_splicing KW - translation_genetic KW - wormbase AU - Maroney, PA AU - Denker, JA AU - Darzynkiewicz, E AU - Laneve, R AU - Nilsen, TW PY - 1995/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012232 ER -