An abundantly expressed mucin-like protein from Toxocara canis infective larvae: the precursor of the larval surface coat glycoproteins. Export

@article{citeulike:3290776, abstract = {Evasion of host immunity by Toxocara canis infective larvae is mediated by the nematode surface coat, which is shed in response to binding by host antibody molecules or effector cells. The major constituent of the coat is the TES-120 glycoprotein series. We have isolated a 730-bp cDNA from the gene encoding the apoprotein precursor of TES-120. The mRNA is absent from T. canis adults but hyperabundant in larvae, making up approximately 10\% of total mRNA, and is trans-spliced with the nematode 5' leader sequence SL1. It encodes a 15.8-kDa protein (after signal peptide removal) containing a typical mucin domain: 86 amino acid residues, 72.1\% of which are Ser or Thr, organized into an array of heptameric repeats, interspersed with proline residues. At the C-terminal end of the putative protein are two 36-amino acid repeats containing six Cys residues, in a motif that can also be identified in several genes in Caenorhabditis elegans. Although TES-120 displays size and charge heterogeneity, there is a single c opy gene and a homogeneous size of mRNA. The association of overexpression of some membrane-associated mucins with immunosuppression and tumor metastasis suggests a possible model for the role of the surface coat in immune evasion by parasitic nematodes.}, author = {Gems, D. H. and Maizels, R. M.}, citeulike-article-id = {3290776}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012240}, journal = {Proceedings of the National Academy of Sciences USA}, keywords = {amino\_acid\_sequence, article, base\_sequence, c\_elegans, caenorhabditis\_elegans, caenorhabditis\_elegans\_genetics, celegans, dna\_complementary\_genetics, dogs, elegans, gene\_expression\_regulation\_developmental, genes\_reiterated, genes\_structural\_helminth, helminth\_proteins\_biosynthesis, helminth\_proteins\_genetics, larva, membrane\_glycoproteins\_biosynthesis, membrane\_glycoproteins\_genetics, molecular\_sequence\_data, nematode, protein\_precursors\_biosynthesis, protein\_precursors\_genetics, protein\_processing\_post\_translational, sequence\_alignment, sequence\_homology\_amino\_acid, signal\_peptides\_metabolism, species\_specificity, toxocara\_canis\_genetics, toxocara\_canis\_growth\_and\_development, toxocara\_canis\_metabolism, wormbase}, pages = {1665--1670}, posted-at = {2008-09-18 22:13:19}, priority = {3}, title = {An abundantly expressed mucin-like protein from Toxocara canis infective larvae: the precursor of the larval surface coat glycoproteins.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012240}, volume = {93}, year = {1996} }

TY - JOUR ID - citeulike:3290776 L3 - citeulike-article-id:3290776 N2 - Evasion of host immunity by Toxocara canis infective larvae is mediated by the nematode surface coat, which is shed in response to binding by host antibody molecules or effector cells. The major constituent of the coat is the TES-120 glycoprotein series. We have isolated a 730-bp cDNA from the gene encoding the apoprotein precursor of TES-120. The mRNA is absent from T. canis adults but hyperabundant in larvae, making up approximately 10% of total mRNA, and is trans-spliced with the nematode 5' leader sequence SL1. It encodes a 15.8-kDa protein (after signal peptide removal) containing a typical mucin domain: 86 amino acid residues, 72.1% of which are Ser or Thr, organized into an array of heptameric repeats, interspersed with proline residues. At the C-terminal end of the putative protein are two 36-amino acid repeats containing six Cys residues, in a motif that can also be identified in several genes in Caenorhabditis elegans. Although TES-120 displays size and charge heterogeneity, there is a single c opy gene and a homogeneous size of mRNA. The association of overexpression of some membrane-associated mucins with immunosuppression and tumor metastasis suggests a possible model for the role of the surface coat in immune evasion by parasitic nematodes. JF - Proceedings of the National Academy of Sciences USA EP - 1670 TI - An abundantly expressed mucin-like protein from Toxocara canis infective larvae: the precursor of the larval surface coat glycoproteins. VL - 93 SP - 1665 KW - amino_acid_sequence KW - article KW - base_sequence KW - c_elegans KW - caenorhabditis_elegans KW - caenorhabditis_elegans_genetics KW - celegans KW - dna_complementary_genetics KW - dogs KW - elegans KW - gene_expression_regulation_developmental KW - genes_reiterated KW - genes_structural_helminth KW - helminth_proteins_biosynthesis KW - helminth_proteins_genetics KW - larva KW - membrane_glycoproteins_biosynthesis KW - membrane_glycoproteins_genetics KW - molecular_sequence_data KW - nematode KW - protein_precursors_biosynthesis KW - protein_precursors_genetics KW - protein_processing_post_translational KW - sequence_alignment KW - sequence_homology_amino_acid KW - signal_peptides_metabolism KW - species_specificity KW - toxocara_canis_genetics KW - toxocara_canis_growth_and_development KW - toxocara_canis_metabolism KW - wormbase AU - Gems, DH AU - Maizels, RM PY - 1996/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012240 ER -