A proline-rich structural protein of the surface sheath of larval Brugia filarial nematode parasites. Export

@article{citeulike:3290785, abstract = {Both cDNA and genomic DNA sequences have been isolated which encode a proline-rich precursor protein of the sheath from microfilariae, the first stage larvae of the filarial nematode parasites Brugia pahangi and Brugia malayi. This 22-kDa protein is soluble only under reducing conditions and is extensively cross-linked by both disulfide and nonreducible bonds. Immunogold electron microscopy shows that the protein is localized exclusively in the sheath, a vestigial remnant of the eggshell, which is retained by and encloses the mature microfilaria. Analysis by Western blotting confirms that the protein is expressed only in microfilariae and adult female worms, although transcripts are detectable only in adult females. The deduced amino acid sequence contains a short N-terminal hydrophobic putative leader sequence, a central repetitive domain that contains 14 copies of a degenerate 5-amino acid repeat with the consensus sequence Met-Pro-Pro-Gln-Gly, and a C-terminal proline-rich domain flanked by clusters of cysteine residues. These clusters can be aligned with cysteine residues implicated in cross-linking of a family of cuticular collagens originally identified in Caenorhabditis elegans but which extends to other nematodes.}, author = {Selkirk, M. E. and Yazdanbakhsh, M. and Freedman, D. and Blaxter, M. L. and Cookson, E. and Jenkins, R. E. and Williams, S. A.}, citeulike-article-id = {3290785}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012273}, journal = {Journal of Biological Chemistry}, keywords = {amino\_acid\_sequence, article, base\_sequence, blotting\_northern, blotting\_western, brugia\_genetics, brugia\_growth\_and\_development, brugia\_physiology, brugia\_ultrastructure, c\_elegans, caenorhabditis\_elegans, celegans, cysteine, electrophoresis\_gel\_two\_dimensional, electrophoresis\_polyacrylamide\_gel, elegans, helminth\_proteins\_genetics, helminth\_proteins\_isolation\_and\_purification, larva, microscopy\_immunoelectron, molecular\_sequence\_data, molecular\_weight, nematode, protein\_precursors\_genetics, protein\_precursors\_isolation\_and\_purification, repetitive\_sequences\_nucleic\_acid, rna\_genetics, rna\_isolation\_and\_purification, sequence\_homology\_nucleic\_acid, signal\_peptides\_genetics, wormbase}, pages = {11002--11008}, posted-at = {2008-09-18 22:16:19}, priority = {3}, title = {A proline-rich structural protein of the surface sheath of larval Brugia filarial nematode parasites.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012273}, volume = {266}, year = {1991} }

TY - JOUR ID - citeulike:3290785 L3 - citeulike-article-id:3290785 N2 - Both cDNA and genomic DNA sequences have been isolated which encode a proline-rich precursor protein of the sheath from microfilariae, the first stage larvae of the filarial nematode parasites Brugia pahangi and Brugia malayi. This 22-kDa protein is soluble only under reducing conditions and is extensively cross-linked by both disulfide and nonreducible bonds. Immunogold electron microscopy shows that the protein is localized exclusively in the sheath, a vestigial remnant of the eggshell, which is retained by and encloses the mature microfilaria. Analysis by Western blotting confirms that the protein is expressed only in microfilariae and adult female worms, although transcripts are detectable only in adult females. The deduced amino acid sequence contains a short N-terminal hydrophobic putative leader sequence, a central repetitive domain that contains 14 copies of a degenerate 5-amino acid repeat with the consensus sequence Met-Pro-Pro-Gln-Gly, and a C-terminal proline-rich domain flanked by clusters of cysteine residues. These clusters can be aligned with cysteine residues implicated in cross-linking of a family of cuticular collagens originally identified in Caenorhabditis elegans but which extends to other nematodes. JF - Journal of Biological Chemistry EP - 11008 TI - A proline-rich structural protein of the surface sheath of larval Brugia filarial nematode parasites. VL - 266 SP - 11002 KW - amino_acid_sequence KW - article KW - base_sequence KW - blotting_northern KW - blotting_western KW - brugia_genetics KW - brugia_growth_and_development KW - brugia_physiology KW - brugia_ultrastructure KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - cysteine KW - electrophoresis_gel_two_dimensional KW - electrophoresis_polyacrylamide_gel KW - elegans KW - helminth_proteins_genetics KW - helminth_proteins_isolation_and_purification KW - larva KW - microscopy_immunoelectron KW - molecular_sequence_data KW - molecular_weight KW - nematode KW - protein_precursors_genetics KW - protein_precursors_isolation_and_purification KW - repetitive_sequences_nucleic_acid KW - rna_genetics KW - rna_isolation_and_purification KW - sequence_homology_nucleic_acid KW - signal_peptides_genetics KW - wormbase AU - Selkirk, ME AU - Yazdanbakhsh, M AU - Freedman, D AU - Blaxter, ML AU - Cookson, E AU - Jenkins, RE AU - Williams, SA PY - 1991/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012273 ER -