Identification of a novel Brugia pahangi beta-tubulin gene (beta 2) and a 22-nucleotide spliced leader sequence on beta 1-tubulin mRNA. Export

@article{citeulike:3290790, abstract = {We have examined the expression of beta-tubulin genes in the parasitic nematode, Brugia pahangi. A genomic library was constructed and screened by hybridization with a Haemonchus contortus beta-tubulin cDNA fragment which recognizes several B. pahangi beta-tubulin sequences, including sequences which correspond to the previously characterized beta 1-tubulin gene. The B. pahangi beta 2-tubulin gene was isolated by selecting clones which hybridize to the H. contortus beta-tubulin gene but which do not hybridize to the beta 1-tubulin gene. A partial sequence of the beta 2-tubulin gene confirms that it codes for a distinct beta-tubulin. Southern hybridization analyses show that the beta 2-tubulin sequence exists as a single copy gene within the B. pahangi genome. Expression of the beta 2-tubulin gene is developmentally regulated and the message is found predominantly in adult male worms, whereas the beta 1-tubulin gene is expressed in microfilariae and approximately equal levels of the transcript are found in male and female adult worms. During mRNA maturation the beta 1-tubulin mRNA of microfilariae and adult worms acquires a trans-spliced leader identical to the SL1 of Caenorhabditis elegans.}, author = {Guenette, S. and Prichard, R. K. and Matlashewski, G.}, citeulike-article-id = {3290790}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012279}, journal = {Molecular and Biochemical Parasitology}, keywords = {amino\_acid\_sequence, article, base\_sequence, blotting\_northern, blotting\_southern, brugia\_genetics, brugia\_growth\_and\_development, c\_elegans, caenorhabditis\_elegans, celegans, dna\_genetics, elegans, gene\_expression\_regulation, genomic\_library, gerbillinae, molecular\_sequence\_data, nematode, polymerase\_chain\_reaction, rna\_messenger\_metabolism, rna\_splicing, sex\_characteristics, tubulin\_genetics, wormbase}, pages = {275--284}, posted-at = {2008-09-18 22:17:31}, priority = {3}, title = {Identification of a novel Brugia pahangi beta-tubulin gene (beta 2) and a 22-nucleotide spliced leader sequence on beta 1-tubulin mRNA.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00012279}, volume = {50}, year = {1992} }

TY - JOUR ID - citeulike:3290790 L3 - citeulike-article-id:3290790 N2 - We have examined the expression of beta-tubulin genes in the parasitic nematode, Brugia pahangi. A genomic library was constructed and screened by hybridization with a Haemonchus contortus beta-tubulin cDNA fragment which recognizes several B. pahangi beta-tubulin sequences, including sequences which correspond to the previously characterized beta 1-tubulin gene. The B. pahangi beta 2-tubulin gene was isolated by selecting clones which hybridize to the H. contortus beta-tubulin gene but which do not hybridize to the beta 1-tubulin gene. A partial sequence of the beta 2-tubulin gene confirms that it codes for a distinct beta-tubulin. Southern hybridization analyses show that the beta 2-tubulin sequence exists as a single copy gene within the B. pahangi genome. Expression of the beta 2-tubulin gene is developmentally regulated and the message is found predominantly in adult male worms, whereas the beta 1-tubulin gene is expressed in microfilariae and approximately equal levels of the transcript are found in male and female adult worms. During mRNA maturation the beta 1-tubulin mRNA of microfilariae and adult worms acquires a trans-spliced leader identical to the SL1 of Caenorhabditis elegans. JF - Molecular and Biochemical Parasitology EP - 284 TI - Identification of a novel Brugia pahangi beta-tubulin gene (beta 2) and a 22-nucleotide spliced leader sequence on beta 1-tubulin mRNA. VL - 50 SP - 275 KW - amino_acid_sequence KW - article KW - base_sequence KW - blotting_northern KW - blotting_southern KW - brugia_genetics KW - brugia_growth_and_development KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - dna_genetics KW - elegans KW - gene_expression_regulation KW - genomic_library KW - gerbillinae KW - molecular_sequence_data KW - nematode KW - polymerase_chain_reaction KW - rna_messenger_metabolism KW - rna_splicing KW - sex_characteristics KW - tubulin_genetics KW - wormbase AU - Guenette, S AU - Prichard, RK AU - Matlashewski, G PY - 1992/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00012279 ER -