A Cell Cycle Regulator in the Germ Line Export

@inproceedings{citeulike:3312704, abstract = {Cyclin dependent kinases control cellular events throughout different stages of the cell cycle. One of these kinases, CDC2, regulates the cell's entry into mitosis. CDC2 itself is regulated by a dual specificity phosphatase called CDC25. This phosphatase removes inhibitory phosphates from CDC2, thus driving the cell into M-phase. Whereas other organisms are known to have between one and three cdc25 genes, four cdc25 homologues have been identified in the C. elegans genome (named cdc-25.1 , cdc-25.2 , cdc-25.3 and cdc-25.4 ). We have described the expression pattern of cdc-25.1 using an affinity purified antibody. CDC-25.1 was localized to maturing oocytes, and was observed in the nuclei and cortical membranes of young embryos. CDC-25.1 was also found in the larval germ line precursor cells, Z2 and Z3. Disruption of cdc-25.1 expression using RNAi resulted in the majority of embryos from injected hermaphrodites failing to hatch. These embryos were aneuploid. Further analysis suggested that this aneuploidy was due to aberrant meioses after the oocytes were fertilized. However, the few embryos treated with RNAi that did hatch developed into agametic Glp-like adults. This same sterile phenotype was observed in a molecular null cdc-25.1 mutant ( nr2036 allele generated by Nemapharm, Inc.). Both hermaphrodites and males were sterile. The somatic gonad appeared to develop normally, but the proliferating germ cells died after the L3 larval stage - a time when wild type germ cells enter meiosis. Transgenes containing wild type copies of cdc-25.1 weakly rescued the sterility. Point mutations were introduced into the CDC-25.1 phosphatase domain and these transgenes behaved like dominant negatives. Further analysis of the cdc-25.1 mutant will be presented at the meeting. Research sponsored by the National Cancer Institute, DHHS, under contract with ABL.}, author = {Ashcroft, N. R. and Golden, A.}, citeulike-article-id = {3312704}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00022966}, journal = {International C. elegans Meeting}, keywords = {c\_elegans, caenorhabditis\_elegans, cdc-251, cdc-252, cdc-253, cdc-254, celegans, elegans, f16b48, k06a57, meeting\_abstract, nematode, r05h52, wormbase, zk63711}, posted-at = {2008-09-22 02:15:45}, priority = {3}, title = {A Cell Cycle Regulator in the Germ Line}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00022966}, year = {1999} }

TY - CONF ID - citeulike:3312704 L3 - citeulike-article-id:3312704 N2 - Cyclin dependent kinases control cellular events throughout different stages of the cell cycle. One of these kinases, CDC2, regulates the cell's entry into mitosis. CDC2 itself is regulated by a dual specificity phosphatase called CDC25. This phosphatase removes inhibitory phosphates from CDC2, thus driving the cell into M-phase. Whereas other organisms are known to have between one and three cdc25 genes, four cdc25 homologues have been identified in the C. elegans genome (named cdc-25.1 , cdc-25.2 , cdc-25.3 and cdc-25.4 ). We have described the expression pattern of cdc-25.1 using an affinity purified antibody. CDC-25.1 was localized to maturing oocytes, and was observed in the nuclei and cortical membranes of young embryos. CDC-25.1 was also found in the larval germ line precursor cells, Z2 and Z3. Disruption of cdc-25.1 expression using RNAi resulted in the majority of embryos from injected hermaphrodites failing to hatch. These embryos were aneuploid. Further analysis suggested that this aneuploidy was due to aberrant meioses after the oocytes were fertilized. However, the few embryos treated with RNAi that did hatch developed into agametic Glp-like adults. This same sterile phenotype was observed in a molecular null cdc-25.1 mutant ( nr2036 allele generated by Nemapharm, Inc.). Both hermaphrodites and males were sterile. The somatic gonad appeared to develop normally, but the proliferating germ cells died after the L3 larval stage - a time when wild type germ cells enter meiosis. Transgenes containing wild type copies of cdc-25.1 weakly rescued the sterility. Point mutations were introduced into the CDC-25.1 phosphatase domain and these transgenes behaved like dominant negatives. Further analysis of the cdc-25.1 mutant will be presented at the meeting. Research sponsored by the National Cancer Institute, DHHS, under contract with ABL. JF - International C. elegans Meeting TI - A Cell Cycle Regulator in the Germ Line KW - c_elegans KW - caenorhabditis_elegans KW - cdc-251 KW - cdc-252 KW - cdc-253 KW - cdc-254 KW - celegans KW - elegans KW - f16b48 KW - k06a57 KW - meeting_abstract KW - nematode KW - r05h52 KW - wormbase KW - zk63711 AU - Ashcroft, NR AU - Golden, A PY - 1999/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00022966 ER -