Reproductive Isolation in Caenorhabditis Export

@inproceedings{citeulike:3316369, abstract = {Reproductive isolation refers to all genetic mechanisms that prevent or limit gene flow between species. The combination of C. elegans (males)/CB5161 (females) is cross-fertile but hybrid animals arrest during embryogenesis. Arrested embryos displayed four distinct terminal phenotypes. 70\% failed to gastrulate. Of these 42\% arrested at the 28-30 cell stage and 28\% continued to divide until approximately 100 cells were present. 30\% of the hybrid embryos did gastrulate. Of these, 18\% arrested at the onset of morphogenesis and 12\% arrested at the two-fold stage. Embryonic lineages were consistent with this arrest profile. In eleven hybrid embryos, no lineage defects were observed prior to the division of the E founder cell. In seven hybrids, either E did not divide (1), or E.a and E.p did not divide (3), or E.a and E.p divided aberrantly along an A-P axis. These embyros failed to gastrulate and arrested either at the 28 cell stage or at the 100 cell stage. In four hybrids, E.a and E.p divided normally. These hybrids gastrulated and arrested either at the onset of morphogenesis (3) or at the two-fold stage (1). In C. elegans , gastrulation defects result from inhibition of zygotic transcription, from defects in endoderm specification, and from mutations in genes with direct effects on gastrulation. Two zygotic genes that exhibit gastrulation-defective phenotypes due to defects in endoderm specification are end-1 and rrs-1 . We are testing these genes for dysgenic interactions in C. elegans /CB5161 hybrids. We also are scoring hybrid embryos for the presence of rhabditin granules as a marker for gut differentiation. Finally, we are using a pes-10::gfp reporter to assess zygotic transcription in hybrid embryos.}, author = {Yen, W. C. and Baird, S. E.}, citeulike-article-id = {3316369}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00023814}, journal = {International C. elegans Meeting}, keywords = {c\_elegans, caenorhabditis\_elegans, celegans, elegans, end-1, f58e102, meeting\_abstract, nematode, pes-10, rrs-1, wormbase, y46g5a27}, posted-at = {2008-09-22 16:11:31}, priority = {3}, title = {Reproductive Isolation in Caenorhabditis}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00023814}, year = {1999} }

TY - CONF ID - citeulike:3316369 L3 - citeulike-article-id:3316369 N2 - Reproductive isolation refers to all genetic mechanisms that prevent or limit gene flow between species. The combination of C. elegans (males)/CB5161 (females) is cross-fertile but hybrid animals arrest during embryogenesis. Arrested embryos displayed four distinct terminal phenotypes. 70% failed to gastrulate. Of these 42% arrested at the 28-30 cell stage and 28% continued to divide until approximately 100 cells were present. 30% of the hybrid embryos did gastrulate. Of these, 18% arrested at the onset of morphogenesis and 12% arrested at the two-fold stage. Embryonic lineages were consistent with this arrest profile. In eleven hybrid embryos, no lineage defects were observed prior to the division of the E founder cell. In seven hybrids, either E did not divide (1), or E.a and E.p did not divide (3), or E.a and E.p divided aberrantly along an A-P axis. These embyros failed to gastrulate and arrested either at the 28 cell stage or at the 100 cell stage. In four hybrids, E.a and E.p divided normally. These hybrids gastrulated and arrested either at the onset of morphogenesis (3) or at the two-fold stage (1). In C. elegans , gastrulation defects result from inhibition of zygotic transcription, from defects in endoderm specification, and from mutations in genes with direct effects on gastrulation. Two zygotic genes that exhibit gastrulation-defective phenotypes due to defects in endoderm specification are end-1 and rrs-1 . We are testing these genes for dysgenic interactions in C. elegans /CB5161 hybrids. We also are scoring hybrid embryos for the presence of rhabditin granules as a marker for gut differentiation. Finally, we are using a pes-10::gfp reporter to assess zygotic transcription in hybrid embryos. JF - International C. elegans Meeting TI - Reproductive Isolation in Caenorhabditis KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - end-1 KW - f58e102 KW - meeting_abstract KW - nematode KW - pes-10 KW - rrs-1 KW - wormbase KW - y46g5a27 AU - Yen, WC AU - Baird, SE PY - 1999/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00023814 ER -