Essential kinase-independent role of a Fer-like non-receptor tyrosine kinase in Caenorhabditis elegans morphogenesis. Export

@article{citeulike:3322364, abstract = {Morphogenesis requires coordination of cell surface activity and cytoskeletal architecture. During the initial stage of morphogenesis in Caenorhabditis elegans, the concerted movement of surface epithelial cells results in enclosure of the embryo by the epidermis. We report that Fer-related kinase-1 (FRK-1), an ortholog of the mammalian non-receptor tyrosine kinase Fer, is necessary for embryonic enclosure and morphogenesis in C. elegans. Expression of FRK-1 in epidermal cells is sufficient to rescue a chromosomal deficiency that removes the frk-1 locus, demonstrating its autonomous requirement in the epidermis. The essential function of FRK-1 is independent of its kinase domain, suggesting a non-enzymatic role in morphogenesis. Localization of FRK-1 to the plasma membrane requires beta-catenin, but not cadherin or alpha-catenin, and muscle-expressed beta-integrin is nonautonomously required for this localization; in the absence of these components FRK-1 becomes nuclear. Mouse FerT rescues the morphogenetic defects of frk-1 mutants and expression of FRK-1 in mammalian cells results in loss of adhesion, implying a conserved function for FRK-1/FerT in cell adhesion and morphogenesis. Thus, FRK-1 performs a kinase-independent function in differentiation and morphogenesis of the C. elegans epidermis during}, author = {Putzke, A. P. and Hikita, S. T. and Clegg, D. O. and Rothman, J. H.}, citeulike-article-id = {3322364}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15958510}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15958510}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00026609}, journal = {Development}, keywords = {article, c\_elegans, caenorhabditis\_elegans, celegans, elegans, elt-1, emb-9, f01g125, frk-1, hmp-1, hmp-2, hmr-1, jac-1, k04h41, k05c46, let-2, nematode, pat-3, r13h44, t04b22, w02b91, w09c21, wormbase, y105c5b21, zk10582}, pages = {3185--3195}, posted-at = {2008-09-23 16:36:54}, priority = {3}, title = {Essential kinase-independent role of a Fer-like non-receptor tyrosine kinase in Caenorhabditis elegans morphogenesis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15958510}, volume = {132}, year = {2005} }

TY - JOUR ID - citeulike:3322364 L3 - citeulike-article-id:3322364 N2 - Morphogenesis requires coordination of cell surface activity and cytoskeletal architecture. During the initial stage of morphogenesis in Caenorhabditis elegans, the concerted movement of surface epithelial cells results in enclosure of the embryo by the epidermis. We report that Fer-related kinase-1 (FRK-1), an ortholog of the mammalian non-receptor tyrosine kinase Fer, is necessary for embryonic enclosure and morphogenesis in C. elegans. Expression of FRK-1 in epidermal cells is sufficient to rescue a chromosomal deficiency that removes the frk-1 locus, demonstrating its autonomous requirement in the epidermis. The essential function of FRK-1 is independent of its kinase domain, suggesting a non-enzymatic role in morphogenesis. Localization of FRK-1 to the plasma membrane requires beta-catenin, but not cadherin or alpha-catenin, and muscle-expressed beta-integrin is nonautonomously required for this localization; in the absence of these components FRK-1 becomes nuclear. Mouse FerT rescues the morphogenetic defects of frk-1 mutants and expression of FRK-1 in mammalian cells results in loss of adhesion, implying a conserved function for FRK-1/FerT in cell adhesion and morphogenesis. Thus, FRK-1 performs a kinase-independent function in differentiation and morphogenesis of the C. elegans epidermis during JF - Development EP - 3195 TI - Essential kinase-independent role of a Fer-like non-receptor tyrosine kinase in Caenorhabditis elegans morphogenesis. VL - 132 SP - 3185 KW - article KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - elt-1 KW - emb-9 KW - f01g125 KW - frk-1 KW - hmp-1 KW - hmp-2 KW - hmr-1 KW - jac-1 KW - k04h41 KW - k05c46 KW - let-2 KW - nematode KW - pat-3 KW - r13h44 KW - t04b22 KW - w02b91 KW - w09c21 KW - wormbase KW - y105c5b21 KW - zk10582 AU - Putzke, AP AU - Hikita, ST AU - Clegg, DO AU - Rothman, JH PY - 2005/// UR - http://view.ncbi.nlm.nih.gov/pubmed/15958510 ER -