A Novel P-granule Protein Required for glh-1 and rde-4 mRNA Accumulation in the Germline Export

@article{citeulike:3325852, abstract = {P granules are germ-cell-specific structures containing RNA and protein and required for proper germ cell development. We are interested in understanding how P granules are assembled or stabilized and whether they regulate mRNA translation or stability in germ cells. Mutations in glh-1, a gene encoding a VASA-like RNA helicase, fail to localize PGL-1 to P granules (Kawasaki et al. Cell 94, 635-645). Using EMS mutagenesis, we identified four mutations in a second gene (Y65B4BL.2) that reduce the accumulation of PGL-1 on P granules. We provisionally refer to this gene as dip-1 (diffuse PGL-1). Like pgl-1 and glh-1, dip-1 mutations cause germline underproliferation and temperature-sensitive maternal-effect sterility. Furthermore, glh-1 mRNA and protein levels are dramatically reduced in dip-1 mutants, suggesting that DIP-1 may be required to produce or stabilize glh-1 mRNAs. DIP-1 is a P-granule-associated protein, raising the exciting possibility that DIP-1 may link P granules to mRNA stability. We investigated this possibility by comparing the genome-wide expression profiles of isolated N2 and dip-1 germlines using microarrays. Although relatively few messages have altered abundances (either increased or decreased) in dip-1 mutant germlines, one gene that shows reduced mRNA accumlation is rde-4. Intriguingly, dip-1 mutants are resistant to pos-1(RNAi) and skn-1(RNAi) by feeding and we speculate that this is caused by a deficiency in RDE-4 protein accumulation in the germline.}, author = {Spike, Caroline and Reinke, Valerie and Strome, Susan}, citeulike-article-id = {3325852}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00027804}, journal = {Development \& Evolution Meeting}, keywords = {c\_elegans, caenorhabditis\_elegans, celegans, deps-1, elegans, glh-1, meeting\_abstract, nematode, pgl-1, t21g53, wormbase, y65b4bl2, zk3814}, posted-at = {2008-09-23 23:17:28}, priority = {3}, title = {A Novel P-granule Protein Required for glh-1 and rde-4 mRNA Accumulation in the Germline}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00027804}, year = {2006} }

TY - JOUR ID - citeulike:3325852 L3 - citeulike-article-id:3325852 N2 - P granules are germ-cell-specific structures containing RNA and protein and required for proper germ cell development. We are interested in understanding how P granules are assembled or stabilized and whether they regulate mRNA translation or stability in germ cells. Mutations in glh-1, a gene encoding a VASA-like RNA helicase, fail to localize PGL-1 to P granules (Kawasaki et al. Cell 94, 635-645). Using EMS mutagenesis, we identified four mutations in a second gene (Y65B4BL.2) that reduce the accumulation of PGL-1 on P granules. We provisionally refer to this gene as dip-1 (diffuse PGL-1). Like pgl-1 and glh-1, dip-1 mutations cause germline underproliferation and temperature-sensitive maternal-effect sterility. Furthermore, glh-1 mRNA and protein levels are dramatically reduced in dip-1 mutants, suggesting that DIP-1 may be required to produce or stabilize glh-1 mRNAs. DIP-1 is a P-granule-associated protein, raising the exciting possibility that DIP-1 may link P granules to mRNA stability. We investigated this possibility by comparing the genome-wide expression profiles of isolated N2 and dip-1 germlines using microarrays. Although relatively few messages have altered abundances (either increased or decreased) in dip-1 mutant germlines, one gene that shows reduced mRNA accumlation is rde-4. Intriguingly, dip-1 mutants are resistant to pos-1(RNAi) and skn-1(RNAi) by feeding and we speculate that this is caused by a deficiency in RDE-4 protein accumulation in the germline. JF - Development & Evolution Meeting TI - A Novel P-granule Protein Required for glh-1 and rde-4 mRNA Accumulation in the Germline KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - deps-1 KW - elegans KW - glh-1 KW - meeting_abstract KW - nematode KW - pgl-1 KW - t21g53 KW - wormbase KW - y65b4bl2 KW - zk3814 AU - Spike, Caroline AU - Reinke, Valerie AU - Strome, Susan PY - 2006/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00027804 ER -