Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germline development and influences histone H3 methylation in Caenorhabditis elegans. Export

@article{citeulike:3328056, abstract = {RNA interference with one of the eight C. elegans linker histones genes triggers desilencing of a repetitive transgene and developmental defects in the hermaphrodite germline. These observations are similar to the phenotype of the C. elegans Polycomb group genes mes-2, mes-3, mes-4, and mes-6 (9, 18). These Polycomb group proteins contribute to germline specific chromatin modifications. Using a his-24 deletion mutant and an isoform specific antibody we characterize the role of his-24 in C. elegans germline development. We describe an unexpected cytoplasmic retention of HIS-24 in peculiar granular structures. This phenomenon is confined to the developing germline of both sexes. It is strictly dependant on the activity of the chromatin modifying genes mes-2, mes-3, mes-4, and mes-6, as well as on the C. elegans sirtuin sir-2.1. A temperature shift experiment with a mes-3(ts) mutant revealed that mes-gene activity is required in a time window ranging from L3 to the early L4 stage before the onset of meiosis. We find that the his-24(ok1024) mutant germline is characterized by an increased level of the activating H3K4 methylation mark concomitant with a decrease of the repressive H3K9 methylation. In the germline of his-24(ok1024) mes-3(bn35) double mutant animals the repressive H3K27 methylation is more reduced than in the respective mes-single -mutant. These observations distinguish his-24 as an unusual element in the developmental regulation of germline chromatin structure in C. elegans.}, author = {Jedrusik, M. A. and Schulze, E.}, citeulike-article-id = {3328056}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17210650}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17210650}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00028983}, journal = {Mol Cell Biol}, keywords = {c09g45, c\_elegans, caenorhabditis\_elegans, celegans, elegans, f54c13, his-24, m1633, mes-2, mes-3, mes-4, mes-6, nematode, other, r06a47, r11a84, sir-21, wormbase, y2h9a1}, pages = {2229--2239}, posted-at = {2008-09-24 04:34:44}, priority = {3}, title = {Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germline development and influences histone H3 methylation in Caenorhabditis elegans.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17210650}, volume = {27}, year = {2007} }

TY - JOUR ID - citeulike:3328056 L3 - citeulike-article-id:3328056 N2 - RNA interference with one of the eight C. elegans linker histones genes triggers desilencing of a repetitive transgene and developmental defects in the hermaphrodite germline. These observations are similar to the phenotype of the C. elegans Polycomb group genes mes-2, mes-3, mes-4, and mes-6 (9, 18). These Polycomb group proteins contribute to germline specific chromatin modifications. Using a his-24 deletion mutant and an isoform specific antibody we characterize the role of his-24 in C. elegans germline development. We describe an unexpected cytoplasmic retention of HIS-24 in peculiar granular structures. This phenomenon is confined to the developing germline of both sexes. It is strictly dependant on the activity of the chromatin modifying genes mes-2, mes-3, mes-4, and mes-6, as well as on the C. elegans sirtuin sir-2.1. A temperature shift experiment with a mes-3(ts) mutant revealed that mes-gene activity is required in a time window ranging from L3 to the early L4 stage before the onset of meiosis. We find that the his-24(ok1024) mutant germline is characterized by an increased level of the activating H3K4 methylation mark concomitant with a decrease of the repressive H3K9 methylation. In the germline of his-24(ok1024) mes-3(bn35) double mutant animals the repressive H3K27 methylation is more reduced than in the respective mes-single -mutant. These observations distinguish his-24 as an unusual element in the developmental regulation of germline chromatin structure in C. elegans. JF - Mol Cell Biol EP - 2239 TI - Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germline development and influences histone H3 methylation in Caenorhabditis elegans. VL - 27 SP - 2229 KW - c09g45 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - f54c13 KW - his-24 KW - m1633 KW - mes-2 KW - mes-3 KW - mes-4 KW - mes-6 KW - nematode KW - other KW - r06a47 KW - r11a84 KW - sir-21 KW - wormbase KW - y2h9a1 AU - Jedrusik, MA AU - Schulze, E PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/17210650 ER -