noah-2: new component in ray morphogenesis of C. elegans. Export

@inproceedings{citeulike:3333807, abstract = { Organ morphogenesis is an important process during development. We use male tail sensory ray as a model and screen for genes that required for this process. From previous forward genetics screens, we identified two novel transmembrane proteins, MAB-7 and RAM-5. Domain analysis of both MAB-7 and RAM-5 proteins showed that their extracellular domains are crucial for their function, therefore the extracellular domains were used as bait in yeast two hybrid screens. A number of potential candidates were identified. The results will be discussed. In addition, a genome-wide screen for interacting partner, targeted RNAi gene knock down was performed to identify potential components required for ray morphogenesis. Since RAM-5 contains a ZP domain curial for protein function and in vitro studies show that ZP proteins interact with each other, we evaluated the biological function of 40 ZP domain-containing proteins in C. elegans ray development. From the screens, we identified noah-2 (NOmpA Homolog, a Drosophila nompA counterpart) shows larval lethal phenotype and Ram phenotype. The gene is expressed in hypodermis from embryo to adult, which explains its requirement in larval development. To confirm the requirement of noah-2 in ray morphogenesis, dominant negative NOAH-2 protein expressing transgenic animals were generated. They display Ram phenotype revealing all features similar to other ram mutants. Furthermore, to test whether noah-2 works together with ram-5 in controlling ray morphogenesis, noah-2 was knocked down in ram-5(bx81) mutant. An enhanced Ram phenotype was observed, suggesting that they are acting synergistically in ray morphogenesis. The implication of this result in the context of ram genes interactions will be presented. (The research is funded by Research Grants Council, Hong Kong.).}, author = {Tsang, Horace S. W. and Hui, Agnes K. Y. and Chow, King L.}, citeulike-article-id = {3333807}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00030652}, journal = {International Worm Meeting}, keywords = {c\_elegans, caenorhabditis\_elegans, celegans, elegans, f52b113, meeting\_abstract, nematode, noah-2, ram-5, t24c21, wormbase}, posted-at = {2008-09-24 20:38:46}, priority = {3}, title = {noah-2: new component in ray morphogenesis of C. elegans.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00030652}, year = {2007} }

TY - CONF ID - citeulike:3333807 L3 - citeulike-article-id:3333807 N2 - Organ morphogenesis is an important process during development. We use male tail sensory ray as a model and screen for genes that required for this process. From previous forward genetics screens, we identified two novel transmembrane proteins, MAB-7 and RAM-5. Domain analysis of both MAB-7 and RAM-5 proteins showed that their extracellular domains are crucial for their function, therefore the extracellular domains were used as bait in yeast two hybrid screens. A number of potential candidates were identified. The results will be discussed. In addition, a genome-wide screen for interacting partner, targeted RNAi gene knock down was performed to identify potential components required for ray morphogenesis. Since RAM-5 contains a ZP domain curial for protein function and in vitro studies show that ZP proteins interact with each other, we evaluated the biological function of 40 ZP domain-containing proteins in C. elegans ray development. From the screens, we identified noah-2 (NOmpA Homolog, a Drosophila nompA counterpart) shows larval lethal phenotype and Ram phenotype. The gene is expressed in hypodermis from embryo to adult, which explains its requirement in larval development. To confirm the requirement of noah-2 in ray morphogenesis, dominant negative NOAH-2 protein expressing transgenic animals were generated. They display Ram phenotype revealing all features similar to other ram mutants. Furthermore, to test whether noah-2 works together with ram-5 in controlling ray morphogenesis, noah-2 was knocked down in ram-5(bx81) mutant. An enhanced Ram phenotype was observed, suggesting that they are acting synergistically in ray morphogenesis. The implication of this result in the context of ram genes interactions will be presented. (The research is funded by Research Grants Council, Hong Kong.). JF - International Worm Meeting TI - noah-2: new component in ray morphogenesis of C. elegans. KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - f52b113 KW - meeting_abstract KW - nematode KW - noah-2 KW - ram-5 KW - t24c21 KW - wormbase AU - Tsang, Horace AU - Hui, Agnes AU - Chow, King PY - 2007/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00030652 ER -