ceh-43/distal-less acts in sensory ray assembly. Export

@inproceedings{citeulike:3333855, abstract = { Although many genes are known to contribute to sensory organ differentiation, the mechanistic operations of them are not well defined. Using the male sensory ray in Caenorhabditis elegans as a model and a missing ray strain KC62, we have defined a bona fide organ assembly process where differentiated ray cells organize themselves as a functional unit and form a ray. The assembly defect in KC62 is caused by high copy number of a transgenic fragment of ram-5 promoter. By deletion analysis on this fragment, a 123 bp region was identified to be responsible for both the missing ray phenotype and the ram-5 reporter expression in ray structural cells. The results infer that this element has sequestered key transcription factors acting in the ray cells. In an RNAi screen of a number of transcription factors required for ray assembly, a distal-less homeobox transcription factor family member, ceh-43, was identified. Two putative Dlx5/distal-less binding sites were subsequently identified in the 123 bp minimal ram-5 promoter responsible for the missing ray phenotype and expression of ram-5 gene. Using a yeast 1-hybrid assay, the direct binding of CEH-43 to the ram-5 promoter has been characterized. ceh-43(RNAi) animals display ray assembly and papillae attachment defects. The temporal and spatial expression of ceh-43 revealed by gfp reporters was defined in the structural cells throughout the whole ray assembly process. The expression of this ceh-43 reporter was further shown to be dependent of lin-32 activity, and ceh-43 was found to control the expression of mab-22 transcriptional reporter. Further characterization of how ceh-43 interacts with these transcriptional regulators is on the way. We hope that the results and identification of its target genes function in governing the ray assembly will allow us to establish a mechanistic understanding of this organ developmental process in C. elegans male. (This study is funded by Research Grants Council, Hong Kong).}, author = {Yip, Brian S. Y. and Yu, Raymond Y. L. and Chow, King L.}, citeulike-article-id = {3333855}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00030671}, journal = {International Worm Meeting}, keywords = {c28a54, c\_elegans, caenorhabditis\_elegans, ceh-43, celegans, elegans, lin-32, mab-22, meeting\_abstract, nematode, ram-5, t14f95, t24c21, wormbase}, posted-at = {2008-09-24 20:58:47}, priority = {3}, title = {ceh-43/distal-less acts in sensory ray assembly.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00030671}, year = {2007} }

TY - CONF ID - citeulike:3333855 L3 - citeulike-article-id:3333855 N2 - Although many genes are known to contribute to sensory organ differentiation, the mechanistic operations of them are not well defined. Using the male sensory ray in Caenorhabditis elegans as a model and a missing ray strain KC62, we have defined a bona fide organ assembly process where differentiated ray cells organize themselves as a functional unit and form a ray. The assembly defect in KC62 is caused by high copy number of a transgenic fragment of ram-5 promoter. By deletion analysis on this fragment, a 123 bp region was identified to be responsible for both the missing ray phenotype and the ram-5 reporter expression in ray structural cells. The results infer that this element has sequestered key transcription factors acting in the ray cells. In an RNAi screen of a number of transcription factors required for ray assembly, a distal-less homeobox transcription factor family member, ceh-43, was identified. Two putative Dlx5/distal-less binding sites were subsequently identified in the 123 bp minimal ram-5 promoter responsible for the missing ray phenotype and expression of ram-5 gene. Using a yeast 1-hybrid assay, the direct binding of CEH-43 to the ram-5 promoter has been characterized. ceh-43(RNAi) animals display ray assembly and papillae attachment defects. The temporal and spatial expression of ceh-43 revealed by gfp reporters was defined in the structural cells throughout the whole ray assembly process. The expression of this ceh-43 reporter was further shown to be dependent of lin-32 activity, and ceh-43 was found to control the expression of mab-22 transcriptional reporter. Further characterization of how ceh-43 interacts with these transcriptional regulators is on the way. We hope that the results and identification of its target genes function in governing the ray assembly will allow us to establish a mechanistic understanding of this organ developmental process in C. elegans male. (This study is funded by Research Grants Council, Hong Kong). JF - International Worm Meeting TI - ceh-43/distal-less acts in sensory ray assembly. KW - c28a54 KW - c_elegans KW - caenorhabditis_elegans KW - ceh-43 KW - celegans KW - elegans KW - lin-32 KW - mab-22 KW - meeting_abstract KW - nematode KW - ram-5 KW - t14f95 KW - t24c21 KW - wormbase AU - Yip, Brian AU - Yu, Raymond AU - Chow, King PY - 2007/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00030671 ER -