An iron enhancer element in the FTN-1 gene directs iron-dependent expression in C. elegans intestine. Export

@article{citeulike:3336106, abstract = {Ferritin is a ubiquitous protein that sequesters iron and protects cells from iron toxicity. Caenorhabditis elegans express two ferritins, FTN-1 and FTN-2, which are transcriptionally regulated by iron. To identify the cis-acting sequences and proteins required for iron-dependent regulation of ftn-1 and ftn-2 expression, we generated transcriptional GFP reporters corresponding to 5'' upstream sequences of the ftn-1 and ftn-2 genes. We identified a conserved 63-bp sequence, the ''iron-dependent element'' (''IDE''), that is required for iron-dependent regulation of a ftn-1 GFP reporter in intestine. The IDE contains two GATA-binding motifs and three octameric direct repeats. Site-specific mutagenesis of the GATA sequences, singly or in combination, reduces ftn-1 GFP reporter expression in the intestine. In vitro DNA mobility shift assays show that the intestine-specific GATA protein ELT-2 binds to both GATA sequences. Inhibition of ELT-2 function by RNA interference blocks ftn-1 GFP reporter expression in vivo. Insertion of the IDE into the promoter region of a heterologous reporter activates iron-dependent transcription in intestine. These data demonstrate that the activation of ftn-1 and ftn-2 transcription by iron requires ELT-2, and that the IDE functions as an iron-dependent enhancer in intestine.}, author = {Romney, S. J. and Thacker, C. and Leibold, E. A.}, citeulike-article-id = {3336106}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18024960}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18024960}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031227}, journal = {J Biol Chem}, keywords = {c33d31, c54f614, c\_elegans, caenorhabditis\_elegans, celegans, d10373, elegans, elt-1, elt-2, elt-6, f52c125, ftn-1, ftn-2, nematode, other, w09c21, wormbase}, posted-at = {2008-09-25 14:59:25}, priority = {3}, title = {An iron enhancer element in the FTN-1 gene directs iron-dependent expression in C. elegans intestine.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18024960}, year = {2007} }

TY - JOUR ID - citeulike:3336106 L3 - citeulike-article-id:3336106 N2 - Ferritin is a ubiquitous protein that sequesters iron and protects cells from iron toxicity. Caenorhabditis elegans express two ferritins, FTN-1 and FTN-2, which are transcriptionally regulated by iron. To identify the cis-acting sequences and proteins required for iron-dependent regulation of ftn-1 and ftn-2 expression, we generated transcriptional GFP reporters corresponding to 5'' upstream sequences of the ftn-1 and ftn-2 genes. We identified a conserved 63-bp sequence, the ''iron-dependent element'' (''IDE''), that is required for iron-dependent regulation of a ftn-1 GFP reporter in intestine. The IDE contains two GATA-binding motifs and three octameric direct repeats. Site-specific mutagenesis of the GATA sequences, singly or in combination, reduces ftn-1 GFP reporter expression in the intestine. In vitro DNA mobility shift assays show that the intestine-specific GATA protein ELT-2 binds to both GATA sequences. Inhibition of ELT-2 function by RNA interference blocks ftn-1 GFP reporter expression in vivo. Insertion of the IDE into the promoter region of a heterologous reporter activates iron-dependent transcription in intestine. These data demonstrate that the activation of ftn-1 and ftn-2 transcription by iron requires ELT-2, and that the IDE functions as an iron-dependent enhancer in intestine. JF - J Biol Chem TI - An iron enhancer element in the FTN-1 gene directs iron-dependent expression in C. elegans intestine. KW - c33d31 KW - c54f614 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - d10373 KW - elegans KW - elt-1 KW - elt-2 KW - elt-6 KW - f52c125 KW - ftn-1 KW - ftn-2 KW - nematode KW - other KW - w09c21 KW - wormbase AU - Romney, SJ AU - Thacker, C AU - Leibold, EA PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/18024960 ER -