qx18 affects cell corpse degradation in C. elegans Export

@inproceedings{citeulike:3337231, abstract = {"Phagocytosis of apoptotic cells is a tightly regulated cellular process by which apoptotic cells are cleared in a rapid, non-inflammatory and histologically undetectable fashion. Genetic studies in C. elegans have identified several genes that function in two partially redundant pathways to regulate cell corpse engulfment, with ced-1, ced-6, ced-7, dyn-1 acting in one pathway and psr-1, ced-2, ced-5, ced-12 and ced-10 in the other. Despite the successful identification of the above genes involved in cell corpse engulfment, many crucial components required for this process are still missing, including genes that are involved in the signal presenting, recognition as well as the degradation of cell corpses. In order to identify additional components that function in the engulfment process, we performed genetic screens and identified qx18, a recessive mutation, which contains many persistent cell corpses at various developmental stages. To determine whether accumulation of cell corpses in qx18 animal is due to a defect in cell corpse engulfment, we performed time-course analysis of cell corpse appearance during development. Significantly higher numbers of cell corpses were observed in the qx18 mutant than that of wild-type animal at all developmental stages, suggesting that qx18 may affect the removal of apoptotic cells. This conclusion was further confirmed by the observation that the cell corpses indeed persisted longer in qx18 animals than in wild-type embryos as revealed by the 4-dimensional microscopy analysis. Furthermore, we found that qx18 affects the degradation rather than the internalization of cell corpses based on acridine orange (AO) staining, which preferentially stains engulfed apoptotic cells and labeled persistent cell corpses in the qx18 mutant. We are currently in the process of cloning the gene affected in the qx18 mutant and performing further phenotypic and genetic analyses to understand its function in the degradation of apoptotic cells. We will report our progress in the meeting."}, author = {Hu, Tianjing and Guo, Pengfei and Wang, Xiaochen}, citeulike-article-id = {3337231}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031731}, keywords = {c02c61, c02f41, c09g128, c48b44, c\_elegans, caenorhabditis\_elegans, ced-1, ced-10, ced-12, ced-2, ced-5, ced-6, ced-7, celegans, dyn-1, elegans, f29b94, f56d27, meeting\_abstract, nematode, psr-1, wormbase, y106g6e5, y41d4b13, y47h9c4}, posted-at = {2008-09-25 17:08:43}, priority = {3}, title = {qx18 affects cell corpse degradation in C. elegans}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031731} }

TY - CONF ID - citeulike:3337231 L3 - citeulike-article-id:3337231 N2 - "Phagocytosis of apoptotic cells is a tightly regulated cellular process by which apoptotic cells are cleared in a rapid, non-inflammatory and histologically undetectable fashion. Genetic studies in C. elegans have identified several genes that function in two partially redundant pathways to regulate cell corpse engulfment, with ced-1, ced-6, ced-7, dyn-1 acting in one pathway and psr-1, ced-2, ced-5, ced-12 and ced-10 in the other. Despite the successful identification of the above genes involved in cell corpse engulfment, many crucial components required for this process are still missing, including genes that are involved in the signal presenting, recognition as well as the degradation of cell corpses. In order to identify additional components that function in the engulfment process, we performed genetic screens and identified qx18, a recessive mutation, which contains many persistent cell corpses at various developmental stages. To determine whether accumulation of cell corpses in qx18 animal is due to a defect in cell corpse engulfment, we performed time-course analysis of cell corpse appearance during development. Significantly higher numbers of cell corpses were observed in the qx18 mutant than that of wild-type animal at all developmental stages, suggesting that qx18 may affect the removal of apoptotic cells. This conclusion was further confirmed by the observation that the cell corpses indeed persisted longer in qx18 animals than in wild-type embryos as revealed by the 4-dimensional microscopy analysis. Furthermore, we found that qx18 affects the degradation rather than the internalization of cell corpses based on acridine orange (AO) staining, which preferentially stains engulfed apoptotic cells and labeled persistent cell corpses in the qx18 mutant. We are currently in the process of cloning the gene affected in the qx18 mutant and performing further phenotypic and genetic analyses to understand its function in the degradation of apoptotic cells. We will report our progress in the meeting." TI - qx18 affects cell corpse degradation in C. elegans KW - c02c61 KW - c02f41 KW - c09g128 KW - c48b44 KW - c_elegans KW - caenorhabditis_elegans KW - ced-1 KW - ced-10 KW - ced-12 KW - ced-2 KW - ced-5 KW - ced-6 KW - ced-7 KW - celegans KW - dyn-1 KW - elegans KW - f29b94 KW - f56d27 KW - meeting_abstract KW - nematode KW - psr-1 KW - wormbase KW - y106g6e5 KW - y41d4b13 KW - y47h9c4 AU - Hu, Tianjing AU - Guo, Pengfei AU - Wang, Xiaochen UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00031731 ER -