A genome-wide RNAi screen for genes that interact with the C. elegans PcG genes sop-2 and sor-3 Export

@inproceedings{citeulike:3337288, abstract = {" Polycomb group (PcG) proteins play an important role in specifying the positional identities of cells by transcriptionally regulating the expression of Hox genes. PcG proteins also function in other processes such as in tumorigenesis and in the self-renewal of stem cells. However, the study of molecular mechanisms of PcG-mediated gene repression in fly and mammals is hindered by the complexity and redundancy of the PcG complexes. We previously showed that C. elegans contains relatively simple PcG complexes (1,2). sop-2 encodes a novel protein that is functionally and structurally related to PH and SCM of the PRC1 PcG complex. sor-3 encodes a novel protein containing an MBT domain, suggesting that it may constitute the PhoRC-like PcG complex in C. elegans. We also demonstrated that mutations in sop-2 cause synthetic lethal in combination of a mutation in sor-1 (encoding a component of the PRC1 complex), sor-3 or mes-6 (encoding a component of the ESC/E(Z) complex). Furthermore, the expression domains of the Hox gene egl-5 were dramatically expanded in the double mutants. The genetic interactions suggest that the distinct PcG complex could function together in gene regulation or, alternatively, that they act in parralle pathways. To further understand the underlying mechanisms of PcG-mediated gene repression, we performed a genome-wide RNAi screen to identify mutants that are synthetic lethal with sop-2 or sor-3 mutants. 70 RNAi clones were identified from 16,540 RNAi clones screened (targeting about 86\% of the genome). We found that a large number of identified genes were previously implicated in chromatin remodeling or in regulating the germline tumor formation. We are further characterizing these putative PcG enhancers. Our study shows that PcG genes interact with multiple chromatin remodeling complexes in regulating the expression of Hox genes. Furthermore, PcG genes function in many biological processes, including vulva formation, specification of dopaminergic and serotonergic neurons and germline tumor formation.1. Zhang et al., Dev. Cell 20032. Zhang et al., Development 2006"}, author = {Tian, Ye and Yang, Dongjia and Li, Zhipeng and Zhang, Hong}, citeulike-article-id = {3337288}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031782}, keywords = {c04e72, c08c31, c09g45, c50e104, c\_elegans, caenorhabditis\_elegans, celegans, egl-5, elegans, meeting\_abstract, mes-6, nematode, sop-2, sor-1, sor-3, wormbase, zk12363}, posted-at = {2008-09-25 17:21:35}, priority = {3}, title = {A genome-wide RNAi screen for genes that interact with the C. elegans PcG genes sop-2 and sor-3}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031782} }

TY - CONF ID - citeulike:3337288 L3 - citeulike-article-id:3337288 N2 - " Polycomb group (PcG) proteins play an important role in specifying the positional identities of cells by transcriptionally regulating the expression of Hox genes. PcG proteins also function in other processes such as in tumorigenesis and in the self-renewal of stem cells. However, the study of molecular mechanisms of PcG-mediated gene repression in fly and mammals is hindered by the complexity and redundancy of the PcG complexes. We previously showed that C. elegans contains relatively simple PcG complexes (1,2). sop-2 encodes a novel protein that is functionally and structurally related to PH and SCM of the PRC1 PcG complex. sor-3 encodes a novel protein containing an MBT domain, suggesting that it may constitute the PhoRC-like PcG complex in C. elegans. We also demonstrated that mutations in sop-2 cause synthetic lethal in combination of a mutation in sor-1 (encoding a component of the PRC1 complex), sor-3 or mes-6 (encoding a component of the ESC/E(Z) complex). Furthermore, the expression domains of the Hox gene egl-5 were dramatically expanded in the double mutants. The genetic interactions suggest that the distinct PcG complex could function together in gene regulation or, alternatively, that they act in parralle pathways. To further understand the underlying mechanisms of PcG-mediated gene repression, we performed a genome-wide RNAi screen to identify mutants that are synthetic lethal with sop-2 or sor-3 mutants. 70 RNAi clones were identified from 16,540 RNAi clones screened (targeting about 86% of the genome). We found that a large number of identified genes were previously implicated in chromatin remodeling or in regulating the germline tumor formation. We are further characterizing these putative PcG enhancers. Our study shows that PcG genes interact with multiple chromatin remodeling complexes in regulating the expression of Hox genes. Furthermore, PcG genes function in many biological processes, including vulva formation, specification of dopaminergic and serotonergic neurons and germline tumor formation.1. Zhang et al., Dev. Cell 20032. Zhang et al., Development 2006" TI - A genome-wide RNAi screen for genes that interact with the C. elegans PcG genes sop-2 and sor-3 KW - c04e72 KW - c08c31 KW - c09g45 KW - c50e104 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - egl-5 KW - elegans KW - meeting_abstract KW - mes-6 KW - nematode KW - sop-2 KW - sor-1 KW - sor-3 KW - wormbase KW - zk12363 AU - Tian, Ye AU - Yang, Dongjia AU - Li, Zhipeng AU - Zhang, Hong UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00031782 ER -