Automated analysis of embryonic gene expression with cellular resolution in C. elegans. Export

@article{citeulike:3337787, abstract = {We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes'' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.}, author = {Murray, J. I. and Bao, Z. and Boyle, T. J. and Boeck, M. E. and Mericle, B. L. and Nicholas, T. J. and Zhao, Z. and Sandel, M. J. and Waterston, R. H.}, citeulike-article-id = {3337787}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18587405}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18587405}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031983}, journal = {Nat Methods}, keywords = {b03041, c18g12, c34e107, c\_elegans, caenorhabditis\_elegans, celegans, cnd-1, elegans, elt-7, end-3, f38a61, f58e105, hlh-1, lit-1, nematode, other, pha-4, w06f121, wormbase}, posted-at = {2008-09-25 18:16:23}, priority = {3}, title = {Automated analysis of embryonic gene expression with cellular resolution in C. elegans.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18587405}, year = {2008} }

TY - JOUR ID - citeulike:3337787 L3 - citeulike-article-id:3337787 N2 - We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes'' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development. JF - Nat Methods TI - Automated analysis of embryonic gene expression with cellular resolution in C. elegans. KW - b03041 KW - c18g12 KW - c34e107 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - cnd-1 KW - elegans KW - elt-7 KW - end-3 KW - f38a61 KW - f58e105 KW - hlh-1 KW - lit-1 KW - nematode KW - other KW - pha-4 KW - w06f121 KW - wormbase AU - Murray, JI AU - Bao, Z AU - Boyle, TJ AU - Boeck, ME AU - Mericle, BL AU - Nicholas, TJ AU - Zhao, Z AU - Sandel, MJ AU - Waterston, RH PY - 2008/// UR - http://view.ncbi.nlm.nih.gov/pubmed/18587405 ER -