Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells Export

@article{citeulike:3269208, abstract = {Ammonia is a cytotoxic factor produced during Helicobacter pylori infection that may reduce the survival of surface epithelial cells. Here we examine whether ammonia kills cells and whether L-glutamine (L-Gln) protects against cell death by stimulating ammonia detoxification pathways. Cell viability and vacuolation were quantified in rat gastric epithelial (RGM1) cells incubated with ammonium chloride at pH 7.4 in the presence or absence of L-Gln. Incubation of RGM1 cells with ammonium chloride caused a dose-dependent increase in cell death and vacuolation, which were both inhibited by L-Gln. We show that RGM1 cells metabolize ammonia to urea via arginase, a process that is stimulated by L-Gln and results in reduced ammonia cytotoxicity. L-Gln also inhibits the uptake and facilitates the extrusion of ammonia from cells. Blockade of glutamine synthetase did not reduce the survival of RGM1 cells, demonstrating that the conversion of L-glutamate and ammonia to L-Gln is not involved in ammonia detoxification. Thus our data support a role for L-Gln and arginase in protection against ammonia-induced cell death in gastric epithelial cells. 10.1152/ajpgi.00235.2002}, author = {Nakamura, Eiji and Hagen, Susan J.}, citeulike-article-id = {3269208}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpgi.00235.2002}, citeulike-linkout-1 = {http://ajpgi.physiology.org/cgi/content/abstract/283/6/G1264}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12388179}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12388179}, day = {1}, doi = {10.1152/ajpgi.00235.2002}, journal = {Am J Physiol Gastrointest Liver Physiol}, keywords = {ammonia, arginase, epithelial\_cells, glutamine}, month = {December}, number = {6}, pages = {G1264--1275}, posted-at = {2008-09-15 15:03:44}, priority = {3}, title = {Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells}, url = {http://dx.doi.org/10.1152/ajpgi.00235.2002}, volume = {283}, year = {2002} }

TY - JOUR ID - citeulike:3269208 L3 - citeulike-article-id:3269208 N2 - Ammonia is a cytotoxic factor produced during Helicobacter pylori infection that may reduce the survival of surface epithelial cells. Here we examine whether ammonia kills cells and whether L-glutamine (L-Gln) protects against cell death by stimulating ammonia detoxification pathways. Cell viability and vacuolation were quantified in rat gastric epithelial (RGM1) cells incubated with ammonium chloride at pH 7.4 in the presence or absence of L-Gln. Incubation of RGM1 cells with ammonium chloride caused a dose-dependent increase in cell death and vacuolation, which were both inhibited by L-Gln. We show that RGM1 cells metabolize ammonia to urea via arginase, a process that is stimulated by L-Gln and results in reduced ammonia cytotoxicity. L-Gln also inhibits the uptake and facilitates the extrusion of ammonia from cells. Blockade of glutamine synthetase did not reduce the survival of RGM1 cells, demonstrating that the conversion of L-glutamate and ammonia to L-Gln is not involved in ammonia detoxification. Thus our data support a role for L-Gln and arginase in protection against ammonia-induced cell death in gastric epithelial cells. 10.1152/ajpgi.00235.2002 IS - 6 JF - Am J Physiol Gastrointest Liver Physiol EP - 1275 TI - Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells VL - 283 SP - G1264 KW - ammonia KW - arginase KW - epithelial_cells KW - glutamine AU - Nakamura, Eiji AU - Hagen, Susan PY - 2002/12/01/ UR - http://dx.doi.org/10.1152/ajpgi.00235.2002 ER -