Anti-inflammatory activity of creatine supplementation in endothelial cells in vitro. Export

@article{citeulike:3475952, abstract = {1 Creatine (CR) supplementation augments muscle strength in skeletal muscle cells by increasing intracellular energy pools. However, the effect of CR supplementation on endothelial cells remains to be clarified. 2 In this study, we investigated whether CR supplementation had any anti-inflammatory activity against human pulmonary endothelial cells in culture. 3 We confirmed that supplementation with 0.5 mM CR significantly increased both intracellular CR and phosphocreatine (PC) through a CR transporter while keeping intracellular ATP levels constant independent of CR supplementation and a CR transporter antagonist. 4 In the assay system of endothelial permeability, supplementation with 5 mM CR significantly suppressed the endothelial permeability induced by serotonin and H(2)O(2). 5 In cell adhesion experiments, supplementation with 5 mM CR significantly suppressed neutrophil adhesion to endothelial cells. 6 In the measurement of adhesion molecules, CR supplementation with more than 0.5 mM CR significantly inhibited the expressions of ICAM-1 and E-selectin on endothelial cells, and the inhibition was significantly suppressed by an adenosine A(2A) receptor antagonist. 7 The present study suggests that CR supplementation has anti-inflammatory activities against endothelial cells.}, author = {Nomura, A. and Zhang, M. and Sakamoto, T. and Ishii, Y. and Morishima, Y. and Mochizuki, M. and Kimura, T. and Uchida, Y. and Sekizawa, K.}, citeulike-article-id = {3475952}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/sj.bjp.0705316}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12812994}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12812994}, doi = {10.1038/sj.bjp.0705316}, issn = {0007-1188}, journal = {British journal of pharmacology}, keywords = {adhesion, atp, cell, creatine, endothelial, h2o2, icam-1, permeability, serotonin}, month = {June}, number = {4}, pages = {715--720}, posted-at = {2008-11-03 17:28:52}, priority = {3}, title = {Anti-inflammatory activity of creatine supplementation in endothelial cells in vitro.}, url = {http://dx.doi.org/10.1038/sj.bjp.0705316}, volume = {139}, year = {2003} }

TY - JOUR ID - citeulike:3475952 L3 - citeulike-article-id:3475952 N2 - 1 Creatine (CR) supplementation augments muscle strength in skeletal muscle cells by increasing intracellular energy pools. However, the effect of CR supplementation on endothelial cells remains to be clarified. 2 In this study, we investigated whether CR supplementation had any anti-inflammatory activity against human pulmonary endothelial cells in culture. 3 We confirmed that supplementation with 0.5 mM CR significantly increased both intracellular CR and phosphocreatine (PC) through a CR transporter while keeping intracellular ATP levels constant independent of CR supplementation and a CR transporter antagonist. 4 In the assay system of endothelial permeability, supplementation with 5 mM CR significantly suppressed the endothelial permeability induced by serotonin and H(2)O(2). 5 In cell adhesion experiments, supplementation with 5 mM CR significantly suppressed neutrophil adhesion to endothelial cells. 6 In the measurement of adhesion molecules, CR supplementation with more than 0.5 mM CR significantly inhibited the expressions of ICAM-1 and E-selectin on endothelial cells, and the inhibition was significantly suppressed by an adenosine A(2A) receptor antagonist. 7 The present study suggests that CR supplementation has anti-inflammatory activities against endothelial cells. IS - 4 JF - British journal of pharmacology SN - 0007-1188 EP - 720 TI - Anti-inflammatory activity of creatine supplementation in endothelial cells in vitro. VL - 139 SP - 715 KW - adhesion KW - atp KW - cell KW - creatine KW - endothelial KW - h2o2 KW - icam-1 KW - permeability KW - serotonin AU - Nomura, A AU - Zhang, M AU - Sakamoto, T AU - Ishii, Y AU - Morishima, Y AU - Mochizuki, M AU - Kimura, T AU - Uchida, Y AU - Sekizawa, K PY - 2003/06// UR - http://dx.doi.org/10.1038/sj.bjp.0705316 ER -