Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.

by: D Pribnow

Proc Natl Acad Sci U S A, Vol. 72, No. 3. (March 1975), pp. 784-788.

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Abstract

Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase.

@article{citeulike:1982833, abstract = {Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase.}, author = {Pribnow, D. }, citeulike-article-id = {1982833}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {binding\_site, cis\_regulatory\_elements, conservation, seminal\_paper}, month = {March}, number = {3}, pages = {784--788}, posted-at = {2007-11-26 00:28:07}, priority = {0}, title = {Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1093168}, volume = {72}, year = {1975} }

RIS record

TY - JOUR ID - citeulike:1982833 L3 - citeulike-article-id:1982833 TI - Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. JF - Proc Natl Acad Sci U S A VL - 72 IS - 3 SP - 784 EP - 788 SN - 0027-8424 N2 - Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase. KW - binding_site KW - cis_regulatory_elements KW - conservation KW - seminal_paper AU - Pribnow, D PY - 1975/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/1093168 ER -

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