Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network Export

@article{citeulike:393088, abstract = {10.1101/gr.234503 The metabolic network in the yeast was reconstructed using currently available genomic, biochemical, and physiological information. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, and transport steps between the compartments and the environment were included. A total of 708 structural open reading frames (ORFs) were accounted for in the reconstructed network, corresponding to 1035 metabolic reactions. Further, 140 reactions were included on the basis of biochemical evidence resulting in a genome-scale reconstructed metabolic network containing 1175 metabolic reactions and 584 metabolites. The number of gene functions included in the reconstructed network corresponds to \^{a}ˆ¼16\% of all characterized ORFs in . Using the reconstructed network, the metabolic capabilities of were calculated and compared with . The reconstructed metabolic network is the first comprehensive network for a eukaryotic organism, and it may be used as the basis for in silico analysis of phenotypic functions. [Supplemental material is available online at . The detailed genome-scale reconstructed model of can be found at or.]}, address = {Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.}, author = {F\~{A}{\P}rster, Jochen and Famili, Iman and Fu, Patrick and Palsson, Bernhard and Nielsen, Jens}, citeulike-article-id = {393088}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.234503}, citeulike-linkout-1 = {http://genome.cshlp.org/content/13/2/244.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/13/2/244.full.pdf}, citeulike-linkout-3 = {http://www.genome.org/cgi/content/abstract/13/2/244}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/12566402}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=12566402}, doi = {10.1101/gr.234503}, issn = {1088-9051}, journal = {Genome Research}, keywords = {biology, genome, genome-scale, metabolic, model, network, organism, reconstruction, systems, template, yeast}, month = {February}, number = {2}, pages = {244--253}, posted-at = {2006-08-01 21:13:46}, priority = {2}, title = {Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network}, url = {http://dx.doi.org/10.1101/gr.234503}, volume = {13}, year = {2003} }

TY - JOUR ID - citeulike:393088 L3 - citeulike-article-id:393088 N2 - 10.1101/gr.234503 The metabolic network in the yeast was reconstructed using currently available genomic, biochemical, and physiological information. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, and transport steps between the compartments and the environment were included. A total of 708 structural open reading frames (ORFs) were accounted for in the reconstructed network, corresponding to 1035 metabolic reactions. Further, 140 reactions were included on the basis of biochemical evidence resulting in a genome-scale reconstructed metabolic network containing 1175 metabolic reactions and 584 metabolites. The number of gene functions included in the reconstructed network corresponds to ∼16% of all characterized ORFs in . Using the reconstructed network, the metabolic capabilities of were calculated and compared with . The reconstructed metabolic network is the first comprehensive network for a eukaryotic organism, and it may be used as the basis for in silico analysis of phenotypic functions. [Supplemental material is available online at . The detailed genome-scale reconstructed model of can be found at or.] IS - 2 JF - Genome Research SN - 1088-9051 AD - Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark. EP - 253 TI - Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network VL - 13 SP - 244 KW - biology KW - genome KW - genome-scale KW - metabolic KW - model KW - network KW - organism KW - reconstruction KW - systems KW - template KW - yeast AU - Förster, Jochen AU - Famili, Iman AU - Fu, Patrick AU - Palsson, Bernhard AU - Nielsen, Jens PY - 2003/02/01/ UR - http://dx.doi.org/10.1101/gr.234503 ER -