Blunting the response to endotoxin in healthy subjects: effects of various doses of intravenous fish oil. Export

@article{citeulike:6007905, abstract = {OBJECTIVE: To test the dose response effect of infused fish oil (FO) rich in n-3 PUFAs on the inflammatory response to endotoxin (LPS) and on membrane incorporation of fatty acids in healthy subjects. DESIGN: Prospective, sequential investigation comparing three different FO doses. SUBJECTS: Three groups of male subjects aged 26.8 +/- 3.2 years (BMI 22.5 +/- 2.1). INTERVENTION: One of three FO doses (Omegaven((R))10\%) as a slow infusion before LPS: 0.5 g/kg 1 day before LPS, 0.2 g/kg 1 day before, or 0.2 g/kg 2 h before. MEASUREMENTS AND RESULTS: Temperature, hemodynamic variables, indirect calorimetry and blood samples (TNF-alpha, stress hormones) were collected. After LPS temperature, ACTH and TNF-alpha concentrations increased in the three groups: the responses were significantly blunted (p < 0.0001) compared with the control group of the Pluess et al. trial. Cortisol was unchanged. Lowest plasma ACTH, TNF-alpha and temperature AUC values were observed after a single 0.2 g/kg dose of FO. EPA incorporation into platelet membranes was dose-dependent. CONCLUSIONS: Having previously shown that the response to LPS was reproducible, this study shows that three FO doses blunted it to various degrees. The 0.2 g/kg perfusion immediately before LPS was the most efficient in blunting the responses, suggesting LPS capture in addition to the systemic and membrane effects.}, author = {Pittet, Yann K. and Berger, Mette M. and Pluess, Thomas-Thi T. and Voirol, Pierre and Revelly, Jean-Pierre P. and Tappy, Luc and Chiol\'{e}ro, Ren\'{e} L.}, citeulike-article-id = {6007905}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00134-009-1689-8}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19844694}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19844694}, day = {21}, doi = {10.1007/s00134-009-1689-8}, issn = {1432-1238}, journal = {Intensive care medicine}, month = {October}, posted-at = {2009-10-25 22:13:50}, priority = {3}, title = {Blunting the response to endotoxin in healthy subjects: effects of various doses of intravenous fish oil.}, url = {http://dx.doi.org/10.1007/s00134-009-1689-8}, year = {2009} }

TY - JOUR ID - citeulike:6007905 L3 - citeulike-article-id:6007905 N2 - OBJECTIVE: To test the dose response effect of infused fish oil (FO) rich in n-3 PUFAs on the inflammatory response to endotoxin (LPS) and on membrane incorporation of fatty acids in healthy subjects. DESIGN: Prospective, sequential investigation comparing three different FO doses. SUBJECTS: Three groups of male subjects aged 26.8 +/- 3.2 years (BMI 22.5 +/- 2.1). INTERVENTION: One of three FO doses (Omegaven((R))10%) as a slow infusion before LPS: 0.5 g/kg 1 day before LPS, 0.2 g/kg 1 day before, or 0.2 g/kg 2 h before. MEASUREMENTS AND RESULTS: Temperature, hemodynamic variables, indirect calorimetry and blood samples (TNF-alpha, stress hormones) were collected. After LPS temperature, ACTH and TNF-alpha concentrations increased in the three groups: the responses were significantly blunted (p < 0.0001) compared with the control group of the Pluess et al. trial. Cortisol was unchanged. Lowest plasma ACTH, TNF-alpha and temperature AUC values were observed after a single 0.2 g/kg dose of FO. EPA incorporation into platelet membranes was dose-dependent. CONCLUSIONS: Having previously shown that the response to LPS was reproducible, this study shows that three FO doses blunted it to various degrees. The 0.2 g/kg perfusion immediately before LPS was the most efficient in blunting the responses, suggesting LPS capture in addition to the systemic and membrane effects. JF - Intensive care medicine SN - 1432-1238 TI - Blunting the response to endotoxin in healthy subjects: effects of various doses of intravenous fish oil. AU - Pittet, Yann AU - Berger, Mette AU - Pluess, Thomas-Thi AU - Voirol, Pierre AU - Revelly, Jean-Pierre AU - Tappy, Luc AU - Chioléro, René PY - 2009/10/21/ UR - http://dx.doi.org/10.1007/s00134-009-1689-8 ER -