Iron accelerates the conversion of dopamine-oxidized intermediates into melanin and provides protection in SH-SY5Y cells.

@article{citeulike:566548, abstract = {Parkinson's disease (PD) is characterized by the selective loss of dopaminergic neurons in the substantia nigra (SN), and it has been suggested that dopamine is one of the main endogenous toxins in the genesis of PD. We demonstrated that thiol antioxidants (the reduced form of glutathione, N-acetyl-L-cysteine, and L-cysteine), which conjugate with one dopamine oxidation intermediate, o-quinone, provided almost complete protection from dopamine-mediated toxicity in SH-SY5Y, a human neuroblastoma cell line. In contrast, catalase partially provided protection against cell death caused by dopamine. These data suggest that the generation of dopamine oxidation intermediates, rather than hydrogen peroxide, plays a pivotal role in dopamine-induced toxicity. Iron accumulated in the SN of patients with PD can cause dopaminergic neuronal degeneration by enhancing oxidative stress. However, we found that iron reduced the total amounts of dopamine oxidation intermediates and enhanced the formation of melanin, a final product of dopamine oxidation. Also, addition of iron inhibited dopamine-induced cytotoxicity. These results suggest that iron can provide protection when it accelerates the conversion of dopamine oxidation intermediates.}, address = {Department of Pharmacology, Graduate School of Pharmaceutical Sciences,Kyoto University, Kyoto, Japan.}, author = {Izumi, Y. and Sawada, H. and Yamamoto, N. and Kume, T. and Katsuki, H. and Shimohama, S. and Akaike, A. }, citeulike-article-id = {566548}, doi = {10.1002/jnr.20595}, issn = {0360-4012}, journal = {J Neurosci Res}, keywords = {dopamine, parkinsons}, month = {October}, number = {1}, pages = {126--137}, posted-at = {2006-03-28 05:58:33}, priority = {3}, title = {Iron accelerates the conversion of dopamine-oxidized intermediates into melanin and provides protection in SH-SY5Y cells.}, url = {http://dx.doi.org/10.1002/jnr.20595}, volume = {82}, year = {2005} }

TY - JOUR ID - citeulike:566548 L3 - citeulike-article-id:566548 TI - Iron accelerates the conversion of dopamine-oxidized intermediates into melanin and provides protection in SH-SY5Y cells. JF - J Neurosci Res VL - 82 IS - 1 SP - 126 EP - 137 AD - Department of Pharmacology, Graduate School of Pharmaceutical Sciences,Kyoto University, Kyoto, Japan. SN - 0360-4012 N2 - Parkinson's disease (PD) is characterized by the selective loss of dopaminergic neurons in the substantia nigra (SN), and it has been suggested that dopamine is one of the main endogenous toxins in the genesis of PD. We demonstrated that thiol antioxidants (the reduced form of glutathione, N-acetyl-L-cysteine, and L-cysteine), which conjugate with one dopamine oxidation intermediate, o-quinone, provided almost complete protection from dopamine-mediated toxicity in SH-SY5Y, a human neuroblastoma cell line. In contrast, catalase partially provided protection against cell death caused by dopamine. These data suggest that the generation of dopamine oxidation intermediates, rather than hydrogen peroxide, plays a pivotal role in dopamine-induced toxicity. Iron accumulated in the SN of patients with PD can cause dopaminergic neuronal degeneration by enhancing oxidative stress. However, we found that iron reduced the total amounts of dopamine oxidation intermediates and enhanced the formation of melanin, a final product of dopamine oxidation. Also, addition of iron inhibited dopamine-induced cytotoxicity. These results suggest that iron can provide protection when it accelerates the conversion of dopamine oxidation intermediates. KW - dopamine KW - parkinsons AU - Izumi, Y AU - Sawada, H AU - Yamamoto, N AU - Kume, T AU - Katsuki, H AU - Shimohama, S AU - Akaike, A PY - 2005/10/01/ UR - http://dx.doi.org/10.1002/jnr.20595 ER -