Neuroprotective effect of cocoa flavonids on in vitro oxidative stress Export

@article{citeulike:3745435, abstract = {Abstract Background\ \ Cocoa is a rich source of flavonoids that, among other functions, can act as antioxidants. In living systems, the production of reactive oxygen species (ROS) activate an array of intracellular cascades, including mitogen-activated protein kinases (MAPK), that are closely associated with cell death or survival pathways. Aim of the study\ \ To ascertain the role of a cocoa extract and its main flavonoid, (-)-epicatechin, in an in vitro model of oxidative stress induced in a neuronal cell line. Methods\ \ We analyzed ROS production by fluorometry (dichlorofluorescein assay), and activation of MAPK pathways including extracellular signal-regulated kinases 1/2 (ERK 1/2), c-Jun N-terminal kinase (JNK), and p-38, by Western blot analysis. Results\ \ Cells incubated with cocoa extract or (-)-epicatechin, reduced ROS production in a dose-dependent manner, reaching 35\% inhibition. pJNK and p38, involved in apoptosis, were down-modulated by cocoa extract and (-)-epicatechin with p38 inhibition reaching up to 70\%. Conclusions\ \ Our results show that cocoa extract and (-)-epicatechin may exert a neuroprotective action by reducing ROS production and modulating MAPK activation.}, author = {Ramiro-Puig, Emma and Casades\'{u}s, Gemma and Lee, Hyoung-Gon and Zhu, Xiongwei and Mcshea, Andrew and Perry, George and P\'{e}rez-Cano, Francisco and Smith, Mark and Castell, Margarida}, citeulike-article-id = {3745435}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00394-008-0761-4}, citeulike-linkout-1 = {http://www.springerlink.com/content/g07524327p175781}, doi = {10.1007/s00394-008-0761-4}, journal = {European Journal of Nutrition}, keywords = {cocoa, neuroprotection, oxidative\_stress, p38mapk}, posted-at = {2008-12-05 01:29:47}, priority = {3}, title = {Neuroprotective effect of cocoa flavonids on in vitro oxidative stress}, url = {http://dx.doi.org/10.1007/s00394-008-0761-4} }

TY - JOUR ID - citeulike:3745435 L3 - citeulike-article-id:3745435 N2 - Abstract Background  Cocoa is a rich source of flavonoids that, among other functions, can act as antioxidants. In living systems, the production of reactive oxygen species (ROS) activate an array of intracellular cascades, including mitogen-activated protein kinases (MAPK), that are closely associated with cell death or survival pathways. Aim of the study  To ascertain the role of a cocoa extract and its main flavonoid, (-)-epicatechin, in an in vitro model of oxidative stress induced in a neuronal cell line. Methods  We analyzed ROS production by fluorometry (dichlorofluorescein assay), and activation of MAPK pathways including extracellular signal-regulated kinases 1/2 (ERK 1/2), c-Jun N-terminal kinase (JNK), and p-38, by Western blot analysis. Results  Cells incubated with cocoa extract or (-)-epicatechin, reduced ROS production in a dose-dependent manner, reaching 35% inhibition. pJNK and p38, involved in apoptosis, were down-modulated by cocoa extract and (-)-epicatechin with p38 inhibition reaching up to 70%. Conclusions  Our results show that cocoa extract and (-)-epicatechin may exert a neuroprotective action by reducing ROS production and modulating MAPK activation. JF - European Journal of Nutrition TI - Neuroprotective effect of cocoa flavonids on in vitro oxidative stress KW - cocoa KW - neuroprotection KW - oxidative_stress KW - p38mapk AU - Ramiro-Puig, Emma AU - Casadesús, Gemma AU - Lee, Hyoung-Gon AU - Zhu, Xiongwei AU - Mcshea, Andrew AU - Perry, George AU - Pérez-Cano, Francisco AU - Smith, Mark AU - Castell, Margarida UR - http://dx.doi.org/10.1007/s00394-008-0761-4 ER -