The promoter region of the human type-I-DNA-topoisomerase gene. Protein-binding sites and sequences involved in transcriptional regulation. Export

@article{citeulike:3740790, abstract = {We examined the promoter of the human type-I-DNA topoisomerase gene (hTOP1) for regions protected against DNase I digestion by nuclear proteins from HeLa or from adenovirus-transformed 293 cells. We identified ten protected DNA sequences within 580 bp of DNA upstream of the transcriptional-start sites and one additional site, which is located between the two clusters of transcriptional-start sites. Several of these protein-binding sites have significant similarities to recognition sequences of known transcription factors including factors Sp1, octamer transcription factor, cAMP-responsive-element-binding protein (CREB/ATF), NF-kappa B and members of the Myc-related family of basic/helix-loop-helix/leucine-zipper proteins. Other protein-binding sites show less or no similarities to known consensus sequences. We investigated the physiological significance of these protein-binding sites using a set of deletion and nucleotide-exchange mutants. We conclude that the expression of the hTOP1 gene is regulated by a complex network of negatively and positively acting transcription factors.}, address = {Division of Biology, University of Konstanz, Germany.}, author = {Heiland, S. and Knippers, R. and Kunze, N.}, citeulike-article-id = {3740790}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8223637}, journal = {European journal of biochemistry / FEBS}, keywords = {coletta, gene, topoisomerase}, month = {November}, number = {3}, pages = {813--822}, posted-at = {2008-12-03 15:12:11}, priority = {2}, title = {The promoter region of the human type-I-DNA-topoisomerase gene. Protein-binding sites and sequences involved in transcriptional regulation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8223637}, volume = {217}, year = {1993} }

TY - JOUR ID - citeulike:3740790 L3 - citeulike-article-id:3740790 N2 - We examined the promoter of the human type-I-DNA topoisomerase gene (hTOP1) for regions protected against DNase I digestion by nuclear proteins from HeLa or from adenovirus-transformed 293 cells. We identified ten protected DNA sequences within 580 bp of DNA upstream of the transcriptional-start sites and one additional site, which is located between the two clusters of transcriptional-start sites. Several of these protein-binding sites have significant similarities to recognition sequences of known transcription factors including factors Sp1, octamer transcription factor, cAMP-responsive-element-binding protein (CREB/ATF), NF-kappa B and members of the Myc-related family of basic/helix-loop-helix/leucine-zipper proteins. Other protein-binding sites show less or no similarities to known consensus sequences. We investigated the physiological significance of these protein-binding sites using a set of deletion and nucleotide-exchange mutants. We conclude that the expression of the hTOP1 gene is regulated by a complex network of negatively and positively acting transcription factors. IS - 3 JF - European journal of biochemistry / FEBS AD - Division of Biology, University of Konstanz, Germany. EP - 822 TI - The promoter region of the human type-I-DNA-topoisomerase gene. Protein-binding sites and sequences involved in transcriptional regulation. VL - 217 SP - 813 KW - coletta KW - gene KW - topoisomerase AU - Heiland, S AU - Knippers, R AU - Kunze, N PY - 1993/November// UR - http://view.ncbi.nlm.nih.gov/pubmed/8223637 ER -