Interplay of DNA supercoiling and catenation during the segregation of sister duplexes Export

@article{citeulike:5806600, abstract = {The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as a result of DNA replication in Escherichia coli cells whose topoisomerase IV activity was inhibited. We combined high-resolution two-dimensional agarose gel electrophoresis with numerical simulations in order to better understand the relationship between the negative supercoiling of DNA generated by DNA gyrase and the DNA interlinking resulting from replication of circular DNA molecules. We showed that in those replication intermediates formed in vivo, catenation and negative supercoiling compete with each other. In interlinked molecules with high catenation numbers negative supercoiling is greatly limited. However, when interlinking decreases, as required for the segregation of newly replicated sister duplexes, their negative supercoiling increases. This observation indicates that negative supercoiling plays an active role during progressive decatenation of newly replicated DNA molecules in vivo. 10.1093/nar/gkp530}, author = {Martinez-Robles, Maria L. and Witz, Guillaume and Hernandez, Pablo and Schvartzman, Jorge B. and Stasiak, Andrzej and Krimer, Dora B.}, citeulike-article-id = {5806600}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp530}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/37/15/5126}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19553196}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19553196}, day = {1}, doi = {10.1093/nar/gkp530}, journal = {Nucl. Acids Res.}, keywords = {coarse\_grained, dna, molecular\_dynamics}, month = {August}, number = {15}, pages = {5126--5137}, posted-at = {2009-09-20 12:07:08}, priority = {2}, title = {Interplay of DNA supercoiling and catenation during the segregation of sister duplexes}, url = {http://dx.doi.org/10.1093/nar/gkp530}, volume = {37}, year = {2009} }

TY - JOUR ID - citeulike:5806600 L3 - citeulike-article-id:5806600 N2 - The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as a result of DNA replication in Escherichia coli cells whose topoisomerase IV activity was inhibited. We combined high-resolution two-dimensional agarose gel electrophoresis with numerical simulations in order to better understand the relationship between the negative supercoiling of DNA generated by DNA gyrase and the DNA interlinking resulting from replication of circular DNA molecules. We showed that in those replication intermediates formed in vivo, catenation and negative supercoiling compete with each other. In interlinked molecules with high catenation numbers negative supercoiling is greatly limited. However, when interlinking decreases, as required for the segregation of newly replicated sister duplexes, their negative supercoiling increases. This observation indicates that negative supercoiling plays an active role during progressive decatenation of newly replicated DNA molecules in vivo. 10.1093/nar/gkp530 IS - 15 JF - Nucl. Acids Res. EP - 5137 TI - Interplay of DNA supercoiling and catenation during the segregation of sister duplexes VL - 37 SP - 5126 KW - coarse_grained KW - dna KW - molecular_dynamics AU - Martinez-Robles, Maria AU - Witz, Guillaume AU - Hernandez, Pablo AU - Schvartzman, Jorge AU - Stasiak, Andrzej AU - Krimer, Dora PY - 2009/08/01/ UR - http://dx.doi.org/10.1093/nar/gkp530 ER -