Automated microscopy system for mosaic acquisition and processing Export

@article{citeulike:699710, abstract = {An automatic mosaic acquisition and processing system for a multiphoton microscope is described for imaging large expanses of biological specimens at or near the resolution limit of light microscopy. In a mosaic, a larger image is created from a series of smaller images individually acquired systematically across a specimen. Mosaics allow wide-field views of biological specimens to be acquired without sacrificing resolution, providing detailed views of biological specimens within context. The system is composed of a fast-scanning, multiphoton, confocal microscope fitted with a motorized, high-precision stage and custom-developed software programs for automatic image acquisition, image normalization, image alignment and stitching. Our current capabilities allow us to acquire data sets comprised of thousands to tens of thousands of individual images per mosaic. The large number of individual images involved in creating a single mosaic necessitated software development to automate both the mosaic acquisition and processing steps. In this report, we describe the methods and challenges involved in the routine creation of very large scale mosaics from brain tissue labelled with multiple fluorescent probes.}, author = {Chow, S. K. and Hakozaki, H. and Price, D. L. and Maclean, N. A. B. and Deerinck, T. J. and Bouwer, J. C. and Martone, M. E. and Peltier, S. T. and Ellisman, M. H.}, citeulike-article-id = {699710}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2818.2006.01577.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/jms/2006/00000222/00000002/art00002}, citeulike-linkout-2 = {http://www3.interscience.wiley.com/cgi-bin/abstract/118727443/ABSTRACT}, doi = {10.1111/j.1365-2818.2006.01577.x}, issn = {0022-2720}, journal = {Journal of Microscopy}, keywords = {alignment, correction, field, fluorescence, image, microscopy, montage, mosaic, multiphoton, normalization, shading, wide}, month = {May}, number = {2}, pages = {76--84}, posted-at = {2009-01-27 17:19:33}, priority = {2}, publisher = {Blackwell Publishing}, title = {Automated microscopy system for mosaic acquisition and processing}, url = {http://dx.doi.org/10.1111/j.1365-2818.2006.01577.x}, volume = {222}, year = {2006} }

TY - JOUR ID - citeulike:699710 L3 - citeulike-article-id:699710 N2 - An automatic mosaic acquisition and processing system for a multiphoton microscope is described for imaging large expanses of biological specimens at or near the resolution limit of light microscopy. In a mosaic, a larger image is created from a series of smaller images individually acquired systematically across a specimen. Mosaics allow wide-field views of biological specimens to be acquired without sacrificing resolution, providing detailed views of biological specimens within context. The system is composed of a fast-scanning, multiphoton, confocal microscope fitted with a motorized, high-precision stage and custom-developed software programs for automatic image acquisition, image normalization, image alignment and stitching. Our current capabilities allow us to acquire data sets comprised of thousands to tens of thousands of individual images per mosaic. The large number of individual images involved in creating a single mosaic necessitated software development to automate both the mosaic acquisition and processing steps. In this report, we describe the methods and challenges involved in the routine creation of very large scale mosaics from brain tissue labelled with multiple fluorescent probes. IS - 2 JF - Journal of Microscopy SN - 0022-2720 EP - 84 TI - Automated microscopy system for mosaic acquisition and processing PB - Blackwell Publishing VL - 222 SP - 76 KW - alignment KW - correction KW - field KW - fluorescence KW - image KW - microscopy KW - montage KW - mosaic KW - multiphoton KW - normalization KW - shading KW - wide AU - Chow, SK AU - Hakozaki, H AU - Price, DL AU - Maclean, NAB AU - Deerinck, TJ AU - Bouwer, JC AU - Martone, ME AU - Peltier, ST AU - Ellisman, MH PY - 2006/05// UR - http://dx.doi.org/10.1111/j.1365-2818.2006.01577.x ER -