Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component Export

@article{dtraSporeEPS, abstract = {10.1073/pnas.0803897105 is a spore-forming bacterium that causes anthrax in humans and in other mammals. The glycoprotein BclA ( collagen-like protein of ) is a major constituent of the exosporium, the outermost surface of spores. The glycosyl part of BclA is an oligosaccharide composed of 2--methyl-4-(3-hydroxy-3-methylbutanamido)-4,6-dideoxy--glucose, referred to as anthrose, and three rhamnose residues. A structure similar to anthrose, 4-(3-hydroxy-3-methylbutanamido)-4,6-dideoxy--glucose is found in the side chain of the capsular polysaccharide (CPS) of spp. MR-4. Under certain growth conditions the bacteria produce a variant CPS lacking one methyl group on the hydroxybutyrate, 4-(3-hydroxybutanamido)-4,6-dideoxy--glucose. Contrary to anthrose, neither of the CPSs is 2- methylated. Here, we report that both CPS variants react with anti- spore sera. We also found that these antisera reacted with flagellae of , reported to be glycosylated with a similar terminal saccharide, 4-(3-hydroxybutanamido)-4,6-dideoxy-2--methyl--glucose. Sera produced by immunization with or cells bound to spores but not to spores in a fluorescent microscopy assay. These experiments show that methylation of the anthrose at the 2 of the sugar ring and at the -3 of 3-hydroxybutyrate are not essential for induction of cross-reactive antibodies. We report the preparation, characterization, and antibody responses to protein conjugates of the two variants of CPS. Both conjugates induced antibodies that bound to both CPS variants by ELISA and to spores, as detected by fluorescent microscopy. We propose the use of CPS conjugates as a component of an anthrax vaccine.}, author = {Kubler-Kielb, Joanna and Vinogradov, Evgeny and Hu, Haijing and Leppla, Stephen H. and Robbins, John B. and Schneerson, Rachel}, citeulike-article-id = {6081126}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0803897105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/25/8709.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/25/8709.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18562275}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18562275}, day = {24}, doi = {10.1073/pnas.0803897105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {adhesion}, month = {June}, number = {25}, pages = {8709--8712}, posted-at = {2009-11-06 15:54:20}, priority = {2}, title = {Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component}, url = {http://dx.doi.org/10.1073/pnas.0803897105}, volume = {105}, year = {2008} }

TY - JOUR ID - dtraSporeEPS L3 - citeulike-article-id:6081126 N2 - 10.1073/pnas.0803897105 is a spore-forming bacterium that causes anthrax in humans and in other mammals. The glycoprotein BclA ( collagen-like protein of ) is a major constituent of the exosporium, the outermost surface of spores. The glycosyl part of BclA is an oligosaccharide composed of 2--methyl-4-(3-hydroxy-3-methylbutanamido)-4,6-dideoxy--glucose, referred to as anthrose, and three rhamnose residues. A structure similar to anthrose, 4-(3-hydroxy-3-methylbutanamido)-4,6-dideoxy--glucose is found in the side chain of the capsular polysaccharide (CPS) of spp. MR-4. Under certain growth conditions the bacteria produce a variant CPS lacking one methyl group on the hydroxybutyrate, 4-(3-hydroxybutanamido)-4,6-dideoxy--glucose. Contrary to anthrose, neither of the CPSs is 2- methylated. Here, we report that both CPS variants react with anti- spore sera. We also found that these antisera reacted with flagellae of , reported to be glycosylated with a similar terminal saccharide, 4-(3-hydroxybutanamido)-4,6-dideoxy-2--methyl--glucose. Sera produced by immunization with or cells bound to spores but not to spores in a fluorescent microscopy assay. These experiments show that methylation of the anthrose at the 2 of the sugar ring and at the -3 of 3-hydroxybutyrate are not essential for induction of cross-reactive antibodies. We report the preparation, characterization, and antibody responses to protein conjugates of the two variants of CPS. Both conjugates induced antibodies that bound to both CPS variants by ELISA and to spores, as detected by fluorescent microscopy. We propose the use of CPS conjugates as a component of an anthrax vaccine. IS - 25 JF - Proceedings of the National Academy of Sciences EP - 8712 TI - Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component VL - 105 SP - 8709 KW - adhesion AU - Kubler-Kielb, Joanna AU - Vinogradov, Evgeny AU - Hu, Haijing AU - Leppla, Stephen AU - Robbins, John AU - Schneerson, Rachel PY - 2008/06/24/ UR - http://dx.doi.org/10.1073/pnas.0803897105 ER -