Glycoproteins of the vitelline envelope of Amphibian oocyte: Biological and molecular characterization of ZPC component (gp41) in Bufo arenarum. Export

@article{citeulike:1133441, abstract = {The vitelline envelope (VE) participates in sperm-egg interactions during the first steps of fertilization. In Bufo arenarum, this envelope is composed of at least four glycoproteins, with molecular masses of 120, 75, 41, and 38 kDa and molar ratio of 1:1.3:7.4:4.8, respectively. These components were isolated and covalently coupled to silanized glass slides in order to study their sperm-binding capacity. When considering the molar ratio of the glycoproteins in the egg-envelope and assuming that each protein is monovalent for sperm, the assay showed that gp41 and gp38 possess 55 and 25\% of total sperm-binding activity. We obtained a full-length cDNA of gp41 (ZPC), comprising a sequence for 486 amino acids, with 43.3\% homology with Xenopus laevis ZPC. As in the case of mammalian ZP3 and Xenopus ZPC, Bufo ZPC presented a furin-like (convertase) and a C-terminal transmembrane domain (TMD) reflecting common biosynthetic and secretory pathways. As it was reported for some fishes, we obtained evidence that suggests the presence of more than one zpc gene in Bufo genome, based on different partial cDNA sequences of zpc, Southern blots and two-dimensional SDS-PAGE of deglycosylated egg-envelope components. As far as we are aware, this is the first observation of the presence of different zpc genes in an Amphibian species. Mol. Reprod. Dev. 74: 629-640, 2007. (c) 2006 Wiley-Liss, Inc.}, address = {Divisi\'{o}n Biolog\'{\i}a del Desarrollo, Instituto de Biolog\'{\i}a Celular y Molecular de Rosario (CONICET‐UNR) and Area Biolog\'{\i}a, Facultad de Ciencias Bioqu\'{\i}micas y Farmac\'{e}uticas, Universidad Nacional de Rosario, Argentina.}, author = {Barisone, Gustavo A A. and Krapf, Dar\'{\i}o and Correa-Fiz, Florencia and Arranz, Silvia E E. and Cabada, Marcelo O O.}, citeulike-article-id = {1133441}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/mrd.20635}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17034049}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17034049}, day = {10}, doi = {10.1002/mrd.20635}, issn = {1040-452X}, journal = {Mol Reprod Dev}, keywords = {amphibian, amphibian-zp, zona-pellucida, zp3, zpc}, month = {October}, number = {5}, pages = {629--640}, posted-at = {2007-03-01 10:24:33}, priority = {2}, title = {Glycoproteins of the vitelline envelope of Amphibian oocyte: Biological and molecular characterization of ZPC component (gp41) in Bufo arenarum.}, url = {http://dx.doi.org/10.1002/mrd.20635}, volume = {74}, year = {2006} }

TY - JOUR ID - citeulike:1133441 L3 - citeulike-article-id:1133441 N2 - The vitelline envelope (VE) participates in sperm-egg interactions during the first steps of fertilization. In Bufo arenarum, this envelope is composed of at least four glycoproteins, with molecular masses of 120, 75, 41, and 38 kDa and molar ratio of 1:1.3:7.4:4.8, respectively. These components were isolated and covalently coupled to silanized glass slides in order to study their sperm-binding capacity. When considering the molar ratio of the glycoproteins in the egg-envelope and assuming that each protein is monovalent for sperm, the assay showed that gp41 and gp38 possess 55 and 25% of total sperm-binding activity. We obtained a full-length cDNA of gp41 (ZPC), comprising a sequence for 486 amino acids, with 43.3% homology with Xenopus laevis ZPC. As in the case of mammalian ZP3 and Xenopus ZPC, Bufo ZPC presented a furin-like (convertase) and a C-terminal transmembrane domain (TMD) reflecting common biosynthetic and secretory pathways. As it was reported for some fishes, we obtained evidence that suggests the presence of more than one zpc gene in Bufo genome, based on different partial cDNA sequences of zpc, Southern blots and two-dimensional SDS-PAGE of deglycosylated egg-envelope components. As far as we are aware, this is the first observation of the presence of different zpc genes in an Amphibian species. Mol. Reprod. Dev. 74: 629-640, 2007. (c) 2006 Wiley-Liss, Inc. IS - 5 JF - Mol Reprod Dev SN - 1040-452X AD - División Biología del Desarrollo, Instituto de Biología Celular y Molecular de Rosario (CONICET‐UNR) and Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina. EP - 640 TI - Glycoproteins of the vitelline envelope of Amphibian oocyte: Biological and molecular characterization of ZPC component (gp41) in Bufo arenarum. VL - 74 SP - 629 KW - amphibian KW - amphibian-zp KW - zona-pellucida KW - zp3 KW - zpc AU - Barisone, Gustavo A AU - Krapf, Darío AU - Correa-Fiz, Florencia AU - Arranz, Silvia E AU - Cabada, Marcelo O PY - 2006/10/10/ UR - http://dx.doi.org/10.1002/mrd.20635 ER -