ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP Export

@article{citeulike:937597, abstract = {ARAP2 is a protein that contains both ArfGAP and RhoGAP domains. We found that it is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP but lacks RhoGAP activity. In agreement with the hypothesis that ARAP2 mediates effects of RhoA, endogenous ARAP2 associated with focal adhesions (FAs) and reduction of ARAP2 expression, by RNAi, resulted in fewer FAs and actin stress fibers (SFs). In cells with reduced levels of endogenous ARAP2, FAs and SFs could be restored with wild-type recombinant ARAP2 but not mutants lacking ArfGAP or Rho-binding activity. Constitutively active Arf6 also caused a loss of SFs. The Rho effector ROK{alpha} was ineffective in restoring FAs. Conversely, overexpression of ARAP2 did not restore SFs in cells treated with a ROK inhibitor but induced punctate accumulations of paxillin. We conclude that ARAP2 is an Arf6GAP that functions downstream of RhoA to regulate focal adhesion dynamics. 10.1242/jcs.03237}, author = {Yoon, Hye-Young and Miura, Koichi and Cuthbert, Jebb E. and Davis, Kathryn K. and Ahvazi, Bijan and Casanova, James E. and Randazzo, Paul A.}, citeulike-article-id = {937597}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.03237}, citeulike-linkout-1 = {http://jcs.biologists.org/cgi/content/abstract/119/22/4650}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17077126}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17077126}, day = {15}, doi = {10.1242/jcs.03237}, journal = {J Cell Sci}, keywords = {arf6}, month = {November}, number = {22}, pages = {4650--4666}, posted-at = {2006-11-09 12:00:28}, priority = {2}, title = {ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP}, url = {http://dx.doi.org/10.1242/jcs.03237}, volume = {119}, year = {2006} }

TY - JOUR ID - citeulike:937597 L3 - citeulike-article-id:937597 N2 - ARAP2 is a protein that contains both ArfGAP and RhoGAP domains. We found that it is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP but lacks RhoGAP activity. In agreement with the hypothesis that ARAP2 mediates effects of RhoA, endogenous ARAP2 associated with focal adhesions (FAs) and reduction of ARAP2 expression, by RNAi, resulted in fewer FAs and actin stress fibers (SFs). In cells with reduced levels of endogenous ARAP2, FAs and SFs could be restored with wild-type recombinant ARAP2 but not mutants lacking ArfGAP or Rho-binding activity. Constitutively active Arf6 also caused a loss of SFs. The Rho effector ROK{alpha} was ineffective in restoring FAs. Conversely, overexpression of ARAP2 did not restore SFs in cells treated with a ROK inhibitor but induced punctate accumulations of paxillin. We conclude that ARAP2 is an Arf6GAP that functions downstream of RhoA to regulate focal adhesion dynamics. 10.1242/jcs.03237 IS - 22 JF - J Cell Sci EP - 4666 TI - ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP VL - 119 SP - 4650 KW - arf6 AU - Yoon, Hye-Young AU - Miura, Koichi AU - Cuthbert, Jebb AU - Davis, Kathryn AU - Ahvazi, Bijan AU - Casanova, James AU - Randazzo, Paul PY - 2006/11/15/ UR - http://dx.doi.org/10.1242/jcs.03237 ER -