Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Export

@article{citeulike:2768975, abstract = {The architecture of human chromosomes in interphase nuclei is still largely unknown. Microscopy studies have indicated that specific regions of chromosomes are located in close proximity to the nuclear lamina (NL). This has led to the idea that certain genomic elements may be attached to the NL, which may contribute to the spatial organization of chromosomes inside the nucleus. However, sequences in the human genome that interact with the NL in vivo have not been identified. Here we construct a high-resolution map of the interaction sites of the entire genome with NL components in human fibroblasts. This map shows that genome-lamina interactions occur through more than 1,300 sharply defined large domains 0.1-10 megabases in size. These lamina-associated domains (LADs) are typified by low gene-expression levels, indicating that LADs represent a repressive chromatin environment. The borders of LADs are demarcated by the insulator protein CTCF, by promoters that are oriented away from LADs, or by CpG islands, suggesting possible mechanisms of LAD confinement. Taken together, these results demonstrate that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus.}, author = {Guelen, L. and Pagie, L. and Brasset, E. and Meuleman, W. and Faza, M. B. and Talhout, W. and Eussen, B. H. and de Klein, A. and Wessels, L. and de Laat, W. and van Steensel, B.}, citeulike-article-id = {2768975}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature06947}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature06947}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18463634}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18463634}, day = {12}, doi = {10.1038/nature06947}, issn = {1476-4687}, journal = {Nature}, keywords = {lamin, lamina, nuclear-envelope, nuclear-localization, nuclear-organization}, month = {June}, number = {7197}, pages = {948--951}, posted-at = {2009-07-06 20:10:20}, priority = {0}, publisher = {Nature Publishing Group}, title = {Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions.}, url = {http://dx.doi.org/10.1038/nature06947}, volume = {453}, year = {2008} }

TY - JOUR ID - citeulike:2768975 L3 - citeulike-article-id:2768975 N2 - The architecture of human chromosomes in interphase nuclei is still largely unknown. Microscopy studies have indicated that specific regions of chromosomes are located in close proximity to the nuclear lamina (NL). This has led to the idea that certain genomic elements may be attached to the NL, which may contribute to the spatial organization of chromosomes inside the nucleus. However, sequences in the human genome that interact with the NL in vivo have not been identified. Here we construct a high-resolution map of the interaction sites of the entire genome with NL components in human fibroblasts. This map shows that genome-lamina interactions occur through more than 1,300 sharply defined large domains 0.1-10 megabases in size. These lamina-associated domains (LADs) are typified by low gene-expression levels, indicating that LADs represent a repressive chromatin environment. The borders of LADs are demarcated by the insulator protein CTCF, by promoters that are oriented away from LADs, or by CpG islands, suggesting possible mechanisms of LAD confinement. Taken together, these results demonstrate that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus. IS - 7197 JF - Nature SN - 1476-4687 EP - 951 TI - Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. PB - Nature Publishing Group VL - 453 SP - 948 KW - lamin KW - lamina KW - nuclear-envelope KW - nuclear-localization KW - nuclear-organization AU - Guelen, L AU - Pagie, L AU - Brasset, E AU - Meuleman, W AU - Faza, MB AU - Talhout, W AU - Eussen, BH AU - de Klein, A AU - Wessels, L AU - de Laat, W AU - van Steensel, B PY - 2008/06/12/ UR - http://dx.doi.org/10.1038/nature06947 ER -