Cutting edge: developmental stage-specific recruitment of cohesin to CTCF sites throughout immunoglobulin loci during B lymphocyte development. Export

@article{citeulike:5161254, abstract = {Contraction of the large Igh and Igkappa loci brings all V genes, spanning >2.5 Mb in each locus, in proximity to DJ(H) or J(kappa) genes. CCCTC-binding factor (CTCF) is a transcription factor that regulates gene expression by long-range chromosomal looping. We therefore hypothesized that CTCF may be crucial for the contraction of the Ig loci, but no CTCF sites have been described in any V loci. Using ChIP-chip, we demonstrated many CTCF sites in the V(H) and V(kappa) regions. However, CTCF enrichment in the Igh locus, but not the Igkappa locus, was largely unchanged throughout differentiation, suggesting that CTCF binding alone cannot be responsible for stage-specific looping. Because cohesin can colocalize with CTCF, we performed chromatin immunoprecipitation for the cohesin subunit Rad21 and found lineage and stage-specific Rad21 recruitment to CTCF in all Ig loci. The differential binding of cohesin to CTCF sites may promote multiple loop formation and thus effective V(D)J recombination.}, author = {Degner, Stephanie C. and Wong, Timothy P. and Jankevicius, Gytis and Feeney, Ann J.}, citeulike-article-id = {5161254}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/19109133}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=19109133}, day = {1}, issn = {1550-6606}, journal = {Journal of immunology (Baltimore, Md. : 1950)}, keywords = {b-cell, b-lymphocyte, cohesin, ctcf, ig, immunoglobulin, rad21}, month = {January}, number = {1}, pages = {44--48}, posted-at = {2009-07-15 19:50:24}, priority = {0}, title = {Cutting edge: developmental stage-specific recruitment of cohesin to CTCF sites throughout immunoglobulin loci during B lymphocyte development.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/19109133}, volume = {182}, year = {2009} }

TY - JOUR ID - citeulike:5161254 L3 - citeulike-article-id:5161254 N2 - Contraction of the large Igh and Igkappa loci brings all V genes, spanning >2.5 Mb in each locus, in proximity to DJ(H) or J(kappa) genes. CCCTC-binding factor (CTCF) is a transcription factor that regulates gene expression by long-range chromosomal looping. We therefore hypothesized that CTCF may be crucial for the contraction of the Ig loci, but no CTCF sites have been described in any V loci. Using ChIP-chip, we demonstrated many CTCF sites in the V(H) and V(kappa) regions. However, CTCF enrichment in the Igh locus, but not the Igkappa locus, was largely unchanged throughout differentiation, suggesting that CTCF binding alone cannot be responsible for stage-specific looping. Because cohesin can colocalize with CTCF, we performed chromatin immunoprecipitation for the cohesin subunit Rad21 and found lineage and stage-specific Rad21 recruitment to CTCF in all Ig loci. The differential binding of cohesin to CTCF sites may promote multiple loop formation and thus effective V(D)J recombination. IS - 1 JF - Journal of immunology (Baltimore, Md. : 1950) SN - 1550-6606 EP - 48 TI - Cutting edge: developmental stage-specific recruitment of cohesin to CTCF sites throughout immunoglobulin loci during B lymphocyte development. VL - 182 SP - 44 KW - b-cell KW - b-lymphocyte KW - cohesin KW - ctcf KW - ig KW - immunoglobulin KW - rad21 AU - Degner, Stephanie AU - Wong, Timothy AU - Jankevicius, Gytis AU - Feeney, Ann PY - 2009/01/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/19109133 ER -