Applying mass spectrometry-based proteomics to genetics, genomics and network biology Export

@article{citeulike:5469872, abstract = {The systematic and quantitative molecular analysis of mutant organisms that has been pioneered by studies on mutant metabolomes and transcriptomes holds great promise for improving our understanding of how phenotypes emerge. Unfortunately, owing to the limitations of classical biochemical analysis, proteins have previously been excluded from such studies. Here we review how technical advances in mass spectrometry-based proteomics can be applied to measure changes in protein abundance, posttranslational modifications and protein-protein interactions in mutants at the scale of the proteome. We finally discuss examples that integrate proteomics data with genomic and phenomic information to build network-centred models, which provide a promising route for understanding how phenotypes emerge.}, author = {Gstaiger, Matthias and Aebersold, Ruedi}, citeulike-article-id = {5469872}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nrg2633}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nrg2633}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19687803}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19687803}, doi = {10.1038/nrg2633}, issn = {1471-0064}, journal = {Nat Rev Genet}, month = {September}, number = {9}, pages = {617--627}, posted-at = {2009-09-04 15:10:07}, priority = {2}, publisher = {Nature Publishing Group}, title = {Applying mass spectrometry-based proteomics to genetics, genomics and network biology}, url = {http://dx.doi.org/10.1038/nrg2633}, volume = {10}, year = {2009} }

TY - JOUR ID - citeulike:5469872 L3 - citeulike-article-id:5469872 N2 - The systematic and quantitative molecular analysis of mutant organisms that has been pioneered by studies on mutant metabolomes and transcriptomes holds great promise for improving our understanding of how phenotypes emerge. Unfortunately, owing to the limitations of classical biochemical analysis, proteins have previously been excluded from such studies. Here we review how technical advances in mass spectrometry-based proteomics can be applied to measure changes in protein abundance, posttranslational modifications and protein-protein interactions in mutants at the scale of the proteome. We finally discuss examples that integrate proteomics data with genomic and phenomic information to build network-centred models, which provide a promising route for understanding how phenotypes emerge. IS - 9 JF - Nat Rev Genet SN - 1471-0064 EP - 627 TI - Applying mass spectrometry-based proteomics to genetics, genomics and network biology PB - Nature Publishing Group VL - 10 SP - 617 AU - Gstaiger, Matthias AU - Aebersold, Ruedi PY - 2009/09// UR - http://dx.doi.org/10.1038/nrg2633 ER -