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	✔ A dual role for EDEM1 in the processing of rod opsin [Quick Edit] [CiTO] Journal of Cell Science, Vol. 122, No. 24. (15 December 2009), pp. 4465-4472, doi:10.1242/jcs.055228 by Maria Kosmaoglou, Naheed Kanuga, Mònica Aguilà, Pere Garriga, Michael E. Cheetham posted by 2 people rasheedastlouis plaissue Abstract Copy My Attachments My Copy Abstract Mutations in rod opsin, the archetypal G-protein-coupled receptor, cause retinitis pigmentosa. The majority of mutations, e.g. P23H, cause protein misfolding, resulting in ER retention, induction of the unfolded protein response and degradation by ERAD. If misfolded rod opsin escapes degradation, it aggregates and forms intracellular inclusions. Therefore, it is important to identify the chaperones that mediate the folding or degradation of rod opsin. ER degradation enhancing α-mannosidase-like 1 (EDEM1) can enhance the release of terminally misfolded glycoproteins from the calnexin chaperone ...
	✔ Aggresome formation and neurodegenerative diseases: therapeutic implications. [Quick Edit] [CiTO] Current medicinal chemistry, Vol. 15, No. 1. (2008), pp. 47-60 by J. A. Olzmann, L. Li, L. S. Chin posted by 2 people rasheedastlouis inbetweener Abstract Copy My Attachments My Copy Abstract Accumulation of misfolded proteins in proteinaceous inclusions is a prominent pathological feature common to many age-related neurodegenerative diseases, including Parkinson's disease, Alzheimer's disease, Huntington's disease, and amyotrophic lateral sclerosis. In cultured cells, when the production of misfolded proteins exceeds the capacity of the chaperone refolding system and the ubiquitin-proteasome degradation pathway, misfolded proteins are actively transported to a cytoplasmic juxtanuclear structure called an aggresome. Aggresome ...
	✔ Direct observation of ligand colocalization on individual receptor molecules. [Quick Edit] [CiTO] Biophysical journal, Vol. 74, No. 5. (May 1998), pp. 2223-2226, doi:10.1016/s0006-3495(98)77931-7 by G. J. Schütz, W. Trabesinger, T. Schmidt posted by 2 people rasheedastlouis plaissue Abstract Copy My Attachments My Copy Abstract We have exploited the novel methodology of far-field fluorescence microscopy at the single molecule level to study colocalization of two different ligand molecules on an individual receptor. The use of dual-wavelength single molecule imaging allows discrimination between isolated and colocalized ligands with an accuracy of 40 nm. In the case of very close proximity of the two ligands, below 7 nm, single pair Forster energy-transfer ...
	✔ Structure and function in rhodopsin. Studies of the interaction between the rhodopsin cytoplasmic domain and transducin. [Quick Edit] [CiTO] Journal of Biological Chemistry, Vol. 267, No. 21. (25 July 1992), pp. 14767-14774 by R. R. Franke, T. P. Sakmar, R. M. Graham, H. G. Khorana posted by 1 person rasheedastlouis Abstract Copy My Attachments My Copy Abstract Structural requirements for the activation of transducin by rhodopsin have been studied by site-specific mutagenesis of bovine rhodopsin. A variety of single amino acid replacements and amino acid insertions and deletions of varying sizes were carried out in the two cytoplasmic loops CD (amino acids 134-151) and EF (amino acids 231-252). Except for deletion mutant delta 137-150, all the mutants bound 11-cis-retinal and displayed normal spectral characteristics. Deletion mutant delta 236-239 in loop EF caused a 50% reduction of transducin activation, ...
	✔ Accumulation of Rhodopsin in Late Endosomes Triggers Photoreceptor Cell Degeneration [Quick Edit] [CiTO] PLoS Genet, Vol. 5, No. 2. (13 February 2009), e1000377, doi:10.1371/journal.pgen.1000377 by Yashodhan Chinchore, Amitavo Mitra, Patrick J. Dolph posted by 2 people rasheedastlouis emptyhb Abstract Copy My Attachments My Copy Abstract Progressive retinal degeneration is the underlying feature of many human retinal dystrophies. Previous work using Drosophila as a model system and analysis of specific mutations in human rhodopsin have uncovered a connection between rhodopsin endocytosis and retinal degeneration. In these mutants, rhodopsin and its regulatory protein arrestin form stable complexes, and endocytosis of these complexes causes photoreceptor cell death. In this study we show that the internalized rhodopsin is not degraded in the lysosome but instead accumulates in the late endosomes. ...
	✔ Why Most Published Research Findings Are False [Quick Edit] [CiTO] PLoS Med, Vol. 2, No. 8. (30 August 2005), e124, doi:10.1371/journal.pmed.0020124 by John P. A. Ioannidis posted by 314 people scarlettb robertlischke austin987 fsesser shoshin pierredv ntsafnat irinas christianmbrodbeck makrehchi balabu jiyu jago cdsouthan DavidG67 shivakmr wwweagle polivares scmelton jphekman Borelli gareth williamrhenson cobi irishoconnor noamross sylvirgro kahenry dullhunk flbarroso xiaoheilong gena pvdg vprieto madhadron Thaverkamp famuvie raftlondon richardmcgee martzirnask sachingarg fergus austin jasonbobe Sciences_communication_societe pengchy vankov ddahlem mikeeck mzkbnt flieder NIlz Bioinformatics - CRUK Journal picks ELMlab cjeans LIBrain lafuente msierk weeks kristinchapman jtcribbs danlurie kentsis raulsierra jasonn paleorthid Tomste StephanMatthiesen fercosber iskanbasal daforerog Worlds of Perception arsyed loopback007 2007lab TeresitaAmezcua fgb RobIncAMDSPhD mwyarbro alexhakkinen thiagomanel chad_davis Aska GustavoLacerda fxdm hkreysa NicolasLescureux v_vinoj jclau nailest LSK olethros yujiangwang martainn apwheele mutesa Danoise freesci virolog orzenil hogneb thomashossie Demeter kevinemamy STS mrt2k9 EPLab oceanblue amargoli hmedal miccoli johntdelaney JoseBrox yochju staceygonzales eyliu Method_Engineering_Services tverwaes indigoviolet CIIR Knowledge Networks Yanno SCCS-iPS yezhenyu jmcarp sudarshaniisc jongrae sebpaquet livingthingdan dejang alhoori dnugent pawelsobko monod MariaChiaraP mawds yockeyc1 ajaymalik davebraze BSUH LKS Journal Club silberbauer shtrom barakplasma jacoboblanco vagantes tatome janosch_kellermann mbas achinerarias notorious_sos mrvaidya DGiles pigironjoe cbg TRHvidsten rossmounce timread BOUKACEM slack---line bibhmos Fneesen chriswillmott biblio24 UCD SILS IS40340 Research Practice inbetweener lynnefox EElliott neverendingaudit brembs davidmackinnon CulCog netzwerkerin FeltonM7675 benoitvalley suizan angelaoliveirapisco Zephyrus gbodner mfenner Anesthesia and Acute Pain mgaldino vikweiz acockfield ulmer InquilineKea zzb3886 systemsmedicine Sergey_gerbek tnhh Hanzhij bhengeveld Malc radoveden Torsten_Holmer ckai1 sona ypjones isp ehluis shangyung mxro klauso GuillaumeFilteau mixedman elsbury mismatched yyfwuhan R_C_Cordeiro feipan egonw lonettma steve3001 jamcar skjq olberd schoeband memming richardh garyfeng guillermina Publicase timhubbard MikolkaMikolka kaniko grolemund calsaleh eraderna richardhudson ErickOrtiz sherdim malkocb pauljaparrigor ellimart lmi_swap ilyashl fbaroni pmcmullen acrmartins girabbit mcartright Stew yangjustinc aarre Horduna JOcumpaugh lhuarotop aaltenburger malvikasharan bpcusack Rohdium lapher abellogin nnavarro Integrated Natural Resources Modelling and Management (INRMM) nicholso sdaehne pratap fuenfgeld jporternj chenlc03 buggle rklancer arne Minimalist__ MurphysLab apgaylard ekrzepka druvus Leho jirak monkare Convention on Knowledge Commons ReadingLab haloin idlegrraphx wmdeneve xmarquez christianholz RobertPollak sunilnarl PaperCollector gingerninja pcarbo dIEMoSIRIS arne_b timflutre helensouth neteler arthurdev gonzalez gbloeb EisenLab mtv renreff tictacgo odopo jmeppley rubenroa LucioAlencar jenniferwilms kubyaddi lord_weldaan Chrismartin76 KGelling banso Najamuddin chris kellychristoffersen spores cami plaissue Imat guhjy Abstract Copy My Attachments My Copy Abstract There is increasing concern that most current published research findings are false. The probability that a research claim is true may depend on study power and bias, the number of other studies on the same question, and, importantly, the ratio of true to no relationships among the relationships probed in each scientific field. In this framework, a research finding is less likely to be true when the studies conducted in a field are smaller; when effect sizes are smaller; when there ...
	✔ How To Choose a Good Scientific Problem [Quick Edit] [CiTO] Molecular Cell, Vol. 35, No. 6. (24 September 2009), pp. 726-728, doi:10.1016/j.molcel.2009.09.013 by Uri Alon posted by 720 people scarlettb robertlischke gotrunc sama535 sobolevnrm kshameer flieder pyjamashark mfrichar atzayacatluspitufresas kangism WeiquanXu alexandreconde claudehy hkimura adailtulima sergiodealencar eduberrocal malkocb martzirnask geoforester songpku Horduna Jansen04 munzmarci RkhBibliotekarien codonusage haiku dtbrookes rettema BOUKACEM ceft PWinter cefa wwweagle plenum renatomilani spongelab haibo031031 golharam derchao OliverHarriman andreasvoellmy yagyav Jporci whijae Chrismartin76 berganza Troolin ge11232002 landolf sandfire kaniko lq408 zhaishuang Gig77 jasonzou scmelton npalma rabit chemistry agyoung arjun_citeulike jessica5263 kaliczp jmzhuo kevinemamy pvriesendorp dgard UWA Science Communication lmountr burakeral nloose tuijajetsu xiaoheilong McCammon ansobol bmoores aprasad friveroll janderz8 maxweizhao lizethgonzalezcarabarn psiquiveth wbsnipes AnthonySoprano Philonski alebalbin annampage cbg cgleaniz TorstenWaldminghaus ommachi ceeskan hairuo flydream0428 simonalpha arpaton kaesmann gonzalez zhouyu aky123 fercosber Schopfel Gorzomagnificent schoeband amynmoore skolaric cynmcc thiago_r grahamc gena yezhenyu isp alun rudalert nicolasunsain rebeccamancy bborgeson akselbst mc1975 LourdesPalacios leonardo cisevol ErickOrtiz ramalldf ruvido R_C_Cordeiro dullhunk tuhinb asm666 smichan krisdye albertomartinez antonio-pgarcia penneyft A_Carrasco Rohdium Med Anthro @ UF nellygidas semillerotelesalud ngosvami karimartins GustavoLacerda floydreed pcalado melo_mcr singhsh dmozhdehi CIIR nbekko aaltenburger viktor21614 herraiz iturtle nellapower jjjpppfff toszko lwaldron chenyx kamilkonowalik dersebu csrocha andreff pvdg edwmac BergmanLab luzmagomezb Adrira flbarroso bgo lukafrei trgibbons laismara sorber thiemehennis Borelli tibbitts rocling kivancuyanik jzm10nyc Posgrado en Ciencias Biomédicas lenahhatem nicolastripaldi chenmengjie87 benoitvalley pjmaybank andreacapocci horus gareth JoseBrox mmborges Mars1986 mogwaing Sergey_gerbek dbikard mittinatten gyparikh langtu1840 wang_rui jhiggins mikeeck yochju dwenig rizal_alejaga davidbindel lmichan welliegirl Diego_Prada clopes2010 silberbauer jerry916 tnhh tomekgrobelski ronaldk SCCS-iPS Sklavit Mnourian guillermina jporternj angect5 nosemeocurrenada jwm Dijoe sgsfak eduardosl girabbit TMichael lyss mariopinedakrch merlinnz Naidel liblab mcartright livingthingdan Torsten_Holmer Publicase MarcoSchmidt rzfrantz dutilh jmchen1011 sd kruyvanna kwoodsend jdiezperezj maniruzzaman yueliu aalibes akuhn krapnik spores 8_01 greenmachine qMike cerkut muratsincan zhujiao Ioana_uva tariqabdulla wdbecker torewig eltonmc LucioAlencar brunofed Perea eisdur abr_pr90 lkakinami avoregnitiv SantoshKalwar ramosbello dejang zchen75 diogomartins snichols15 installe dill2009 marsl0am mthomure bertelsen Quotato hyunjin4jc kvalyi czarfv nandipati yetun sdaehne roys cardiffuni fheintz joliveirinha kunefge majdula Sal zalabani kirchengast timflutre ajstuapt icq242500260 cpeters tux2000 lurixea vallinder jcastillod milab@yzu megraw thiagomanel rumig aubinhon mahlow mcc19732001 poojamaj lynnefox yreich ghunter jsvoboda elsantosneto coleslaw ecircuit Layla-Renata grahampoulter chadgh seneca Dori2008 birukou bmsousa sriesenfeld Edwards Lab - molecular evolution & bioinformatics rubystallion hiec hollace abelibanez rcardoso snakehsu ddbb12 trolles wmspringer jwboy nailest socialSurfing mgaldino ff 6065002 Asclepius robfsouza wiizane wenhaosparty jichok daisukekomura garwood operon gstan atlantis2360 drmacgregor tamabravolillo Journal picks marycruzgomez reifmegan lp2 christianharris darshan iman_tavassoly bibhmos michaelbarton cabbagesofdoom GuillaumeFilteau dominique0705 sengelken leomoraes kees JoeVinegar kasianczuk Integrative_biology_behavior djproctor billhao yza0008228 ajaymalik mattbiehl zhangfh rogermug makrehchi leliavski assilva wuchangsong weeks kellychristoffersen CIBERLITERATURA UNAM karthikraman binghe karelvdv wilvancleve cbniles darian fgibson rrbarb icecrown krbanks Hanzhij hlustig kinestetika lzavala sby jirak dengjie robfree henningninneh petrposik alhoori plaissue ianwelch nanonan jtcribbs phreeza gfacciol lgedraite fergus mcartwri tellis Information Networks and Knowledge Management xiaokeeie Cavor hax123 applebyb hamannj netzwerkerin artaban421 eudominguezmartin owenlancaster Kaihami Bobbin isabelpinho GregJordan khpark firasofting chenlc03 peteshadbolt masmavituna lispler kvjanos lilianvt banus nickvandewiele istoyanov ypjones Gobbledygook mfenner phildeley druvus mtaifi rschaeff intelchen krisl AxelS chburrus herrk edws rvasa iammealva tmaillart williamrhenson oolfanska makukha ilyashl paulschlesinger viren4388 ckcheng Thaverkamp loopback007 asadcbt pajoma aldra claudiushauptmann shaw6955 richardhwest hmedal MV_KUL kokphinchooi kou_jinsei mculbert vprieto pgardois jdreyf Shared Literature Resource of the Microbial Fitness Group - MPI Bremen ayansamanta timread jalonsoarevalo nyarkohpeter briancpotter leepro chvlyl malvikasharan daforerog doraemonmaggie kizzie wmdeneve eesruiz allaves collier12 zhouwenbiao ishiken tahsin VisionLab fox201 sandyglobin en216 MartinD gvdh andreassorge meriemelkaroui emonroy abellogin myriasofo skywalkerus02 pdpcosta azwinkau marianp sachingarg tacocohen wangngu iskanbasal ExtremeTomato barry msteffen1392 jdoconnell _mkeller lijiejacy gego TeresitaAmezcua saramovahedi cathrinhyde rezash anuragsethi tarokiritani jrmacias krzstefaniak joshsziegler ZXMYST starless UAB Human Behavioral Neuroscience mcy rabio gjuggler ntsafnat zahreddin duel_jetty mmwoodman samjlord jschmidt fercisob henlimbai migee chriswillmott cgravlee goborobo m_lew myui sbarbit ihuston danlurie davidmam sfalsharif dna beshining moernerlab crlfr fgysin Personal interest huskeypm Avefenix Abero sandy19 zzb3886 flaviooliveirafrancisco sudarshaniisc yangjustinc josephjose Faerk skjq guhjy ct586 gdauria pullus Jorgelp rodney tonyralmeida shtrom NIlz fuenfgeld nbhammond digitaldingo arthurdev probinson vabresp schmidtm dmorti renatazarate MHRashid julianyl darwininseattle anallam christianholz shangyung annehilde mzabidi kozima arinbasu dianella mlee1 samiahk zouchen621 rdinnage GeeSharpMinor richardmcgee yendermclee Danoise ehluis pem galvareza10 flashton cm1acb daveGerrard agong xawen henning elvinado psique niallhaslam akreh yongliwang pigironjoe tiagoferreto Xusheng glynj yyfwuhan gatamir aip fierykylin kochungt WAF flips mordiano afcallender jeffcrow PollardWall coleoguy Mikaela321 jarekadam snezana srimandayam leocmcheung cxshang mblind christineK italiamercado mismatched toates kristina cbonfil npotters xavig wamserma oanapaun replacement phoenixzxl RobertSOakes edutars H-Arrietaumn irishbabe17k mxro sona erisen mikezburke aarre irishoconnor jasonn mwd kubyaddi doc_solitude konilovsky josefranco vschoefer JeremyZucker ama600 Brain-like research group lukeb musil cswarth lawa lesandrop realfsen mkrishna lxm1117 olethros momo54 junehlee peony LucaPappalardoPhD dmeister iannis limbu bayesian CulCog chrisd noamross rakitin jodeleeuw ibre robertporsch sspela EneidaH avilagalfredo yuifu cermak mariana_ma99 yeminjiao datom luciensamary michaelbanks acockfield mmuecke Abstract Copy My Attachments My Copy Abstract Choosing good problems is essential for being a good scientist. But what is a good problem, and how do you choose one? The subject is not usually discussed explicitly within our profession. Scientists are expected to be smart enough to figure it out on their own and through the observation of their teachers. This lack of explicit discussion leaves a vacuum that can lead to approaches such as choosing problems that can give results that merit publication in valued journals, resulting ...
	✔ Contrast, resolution, pixelation, dynamic range and signal-to-noise ratio: fundamental limits to resolution in fluorescence light microscopy [Quick Edit] [CiTO] Journal of Microscopy, Vol. 189, No. 1. (1998), pp. 15-24, doi:10.1046/j.1365-2818.1998.00290.x by Stelzer posted by 7 people willie_gt sjysst plaissue moernerlab lechristophe samjlord ashko Abstract Copy My Attachments My Copy Abstract In a perfect optical system numerical aperture and wavelength determine resolution. In a real optical system, however, the number of photons collected from a specimen determines the contrast and this limits the resolution. Contrast is affected by the number of picture elements per unit area, the number of photons and the aberrations present in every optical system. The concept of contrast vs. distance functions is used to compare the resolution achievable in confocal and wide-field fluorescence microscopes and the effect of ...
	✔ Colocalization of fluorescent markers in confocal microscope images of plant cells [Quick Edit] [CiTO] Nature Protocols, Vol. 3, No. 4. (20 March 2008), pp. 619-628, doi:10.1038/nprot.2008.31 by Andrew P. French, Steven Mills, Ranjan Swarup, Malcolm J. Bennett, Tony P. Pridmore posted by 5 people guhjy kedmond g-cianci mox plaissue Abstract Copy My Attachments My Copy Abstract This protocol describes the steps needed to perform quantitative statistical colocalization on two-color confocal images, specifically of plant cells. The procedure includes a calibration test to check the chromatic alignment of the confocal microscope. A software tool is provided to calculate the Pearson and Spearman correlation coefficients ('Pearson–Spearman correlation colocalization' ImageJ plug-in) across regions of interest within the image. Steps are included to help the user practice using the software. The result is a quantitative estimate of the amount of colocalization ...
	✔ On/off blinking and switching behaviour of single molecules of green fluorescent protein. [Quick Edit] [CiTO] Nature, Vol. 388, No. 6640. (24 July 1997), pp. 355-358, doi:10.1038/41048 by R. M. Dickson, A. B. Cubitt, R. Y. Tsien, W. E. Moerner posted by 7 people plaissue matthieu_b samjlord yanjiang rodney moernerlab Kopelman_Group Abstract Copy My Attachments My Copy Abstract Optical studies of individual molecules at low and room temperature can provide information about the dynamics of local environments in solids, liquids and biological systems unobscured by ensemble averaging. Here we present a study of the photophysical behaviour of single molecules of the green fluorescent protein (GFP) derived from the jellyfish Aequorea victoria. Wild-type GFP and its mutant have attracted interest as fluorescent biological labels because the fluorophore may be formed in vivo. GFP mutants immobilized in aereated aqueous polymer gels ...
	✔ Spherical nanosized focal spot unravels the interior of cells [Quick Edit] [CiTO] Nature Methods, Vol. 5, No. 6. (18 May 2008), pp. 539-544, doi:10.1038/nmeth.1214 by Roman Schmidt, Christian A. Wurm, Stefan Jakobs, Johann Engelhardt, Alexander Egner, Stefan W. Hell posted by 7 people marissalee plaissue alexknight samjlord moernerlab melting lechristophe Abstract Copy My Attachments My Copy Abstract The resolution of any linear imaging system is given by its point spread function (PSF) that quantifies the blur of an object point in the image. The sharper the PSF, the better the resolution is. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot, which extends over at least half the wavelength of light ( = 400–700 nm) in the focal plane and > along the optical axis (z). Although concepts have ...
	✔ Light microscopy techniques for live cell imaging. [Quick Edit] [CiTO] Science (New York, N.Y.), Vol. 300, No. 5616. (4 April 2003), pp. 82-86, doi:10.1126/science.1082160 by David J. Stephens, Victoria J. Allan posted by 15 people devinli plaissue kdouglass Sese schmidtm OliverHarriman abhishek_tiwari gabe_rosser marissalee ts3435 lmcf cherrie samjlord electricsbm moernerlab Abstract Copy My Attachments My Copy Abstract Since the earliest examination of cellular structures, biologists have been fascinated by observing cells using light microscopy. The advent of fluorescent labeling technologies plus the plethora of sophisticated light microscope techniques now available make studying dynamic processes in living cells almost commonplace. For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to ...
	✔ Four stages of a scientific discipline; four types of scientist. [Quick Edit] [CiTO] Trends in biochemical sciences, Vol. 34, No. 5. (09 May 2009), pp. 217-223, doi:10.1016/j.tibs.2009.02.002 by Alexander M. Shneider posted by 98 people yochju kevinemamy yyfwuhan cgleaniz nailest TeresitaAmezcua plaissue barry marclijour xiaoheilong quantumbiology jirak ParisMontagne GustavoLacerda benoitvalley memerman FAB-lab mrvaidya BergmanLab artaban421 lxm1117 TMichael ajaymalik yezhenyu ErickOrtiz williamrhenson skjq lynnefox guhjy rvosa hmedal andreassorge svenboekhoff banso Centre de Recherches Interdisciplinaires Horduna vprieto fuenfgeld spores qwermish dullhunk polivares sfalsharif Gorzomagnificent tomhebbron netzwerkerin mfrichar mismatched JoseBrox jtcribbs timflutre ClaireTruffinet parismontagne cbg Demeter zzb3886 SCCS-iPS mariopinedakrch trolles chemhackerutm elvinado rossmounce irishoconnor flbarroso noamross mordiano pralexation timread GuillaumeFilteau Rohdium mfenner lmichan iskanbasal agomez chriswillmott freesci reflist singhsh Sergey_gerbek plosmedicine daforerog CulCog yangjustinc abhishek_tiwari operon brembs ommachi Ryerson U. MMSc druvus Enro coela cisevol McCammon reyez silberbauer Zephyrus fgibson neils Abstract Copy My Attachments My Copy Abstract In this article I propose the classification of the evolutionary stages that a scientific discipline evolves through and the type of scientists that are the most productive at each stage. I believe that each scientific discipline evolves sequentially through four stages. Scientists at stage one introduce new objects and phenomena as subject matter for a new scientific discipline. To do this they have to introduce ...
	✔ Single-molecule mountains yield nanoscale cell images [Quick Edit] [CiTO] Nature Methods, Vol. 3, No. 10. (01 October 2006), pp. 781-782, doi:10.1038/nmeth1006-781 by W. E. Moerner posted by 5 people marissalee plaissue alexknight samjlord moernerlab Abstract Copy My Attachments My Copy Abstract Methods to simultaneously localize the positions of multiple single fluorophores by precisely determining their individual positions are now yielding impressive gains in fluorescence microscopy resolution. Optical microscopy has been a mainstay of cellular biology ever since van Leeuwenhoek used Hooke's microscope to observe the strange biological objects he called “animalcules.” Using light for biological microscopy takes advantage of its relatively noninvasive character, and with high magnification one can work at a distance, observing small objects. ...
	✔ Microscopy and its focal switch [Quick Edit] [CiTO] Nature Methods, Vol. 6, No. 1. (17 December 2008), pp. 24-32, doi:10.1038/nmeth.1291 by Stefan W. Hell posted by 7 people OliverHarriman meriemelkaroui plaissue alexknight moernerlab samjlord marissalee Abstract Copy My Attachments My Copy Abstract Until not very long ago, it was widely accepted that lens-based (far-field) optical microscopes cannot visualize details much finer than about half the wavelength of light. The advent of viable physical concepts for overcoming the limiting role of diffraction in the early 1990s set off a quest that has led to readily applicable and widely accessible fluorescence microscopes with nanoscale spatial resolution. Here I discuss the principles of these methods together with their differences in implementation and operation. Finally, I outline ...
	✔ Putting super-resolution fluorescence microscopy to work [Quick Edit] [CiTO] Nature Methods, Vol. 6, No. 1. (17 December 2008), pp. 21-23, doi:10.1038/nmeth.f.233 by Jennifer Lippincott-Schwartz, Suliana Manley posted by 6 people alexknight mousearms plaissue meriemelkaroui moernerlab samjlord Abstract Copy My Attachments My Copy Abstract Super-resolution microscopy is poised to revolutionize our understanding of the workings of the cell. But the technology still has some limitations, and these must be taken into consideration if widespread application is to yield biological insight. Light microscopy has been a favorite tool of biologists for unlocking life's mysteries since Leeuwenhoek first focused light through a lens to study living microorganisms, or “animalcules,” in the seventeenth century. Because of Leeuwenhoek's work and that of his successors, we know much about the ...
	✔ An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. [Quick Edit] [CiTO] Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 20. (1 October 2002), pp. 12651-12656, doi:10.1073/pnas.202320599 by Ryoko Ando, Hiroshi Hama, Miki Yamamoto-Hino, Hideaki Mizuno, Atsushi Miyawaki posted by 6 people moernerlab samjlord ts3435 mousearms marissalee plaissue Abstract Copy My Attachments My Copy Abstract We have cloned a gene encoding a fluorescent protein from a stony coral, Trachyphyllia geoffroyi, which emits green, yellow, and red light. The protein, named Kaede, includes a tripeptide, His-Tyr-Gly, that acts as a green chromophore that can be converted to red. The red fluorescence is comparable in intensity to the green and is stable under usual aerobic conditions. We found that the green-red conversion ...
	✔ Real Lives and White Lies in the Funding of Scientific Research [Quick Edit] [CiTO] PLoS Biol, Vol. 7, No. 9. (15 September 2009), e1000197, doi:10.1371/journal.pbio.1000197 by Peter A. Lawrence posted by 98 people yochju daveGerrard rodney barry Zephyrus spongelab kevinchannon polivares Caronte samjlord nGrenier banso MurphysLab dullhunk henlimbai jshigh tnhh chriswillmott rossmounce ppgardne fuenfgeld seb1 druvus silberbauer jago Mycology ntsafnat hmedal farhat aldra mkrishna NIlz renatomilani svenboekhoff jtcribbs Yanno ArtemPankin Torsten_Holmer mittinatten JOcumpaugh cbg Journal picks LolaOyebode flieder cgleaniz cucurbituril ifiokoye BergmanLab maren dmnapolitano McCammon JLab benoitvalley karthikraman 8_01 chrisd hawksjohnd rdiaz stajich williamrhenson ommachi lilith reyez nuin ePortfolio Convention on Knowledge Commons ubcg08l tsjipko Horduna sfalsharif richardhudson Rohdium GuillaumeFilteau spores jasonn mawds lmichan mrvaidya tomhebbron jirak mfenner plaissue andreassorge zzb3886 jclau cisevol ahmetbakan JoseBrox H-Arrietaumn Geknitics Laurentjp CulCog ngehlenborg eisdur Borelli ErickOrtiz MarcoSchmidt lynnefox Copy My Attachments My Copy
	✔ Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics [Quick Edit] [CiTO] Nature Methods, Vol. 5, No. 5. (13 April 2008), pp. 417-423, doi:10.1038/nmeth.1202 by Hari Shroff, Catherine G. Galbraith, James A. Galbraith, Eric Betzig posted by 6 people mousearms plaissue alexknight samjlord moernerlab melting Abstract Copy My Attachments My Copy Abstract We demonstrate live-cell super-resolution imaging using photoactivated localization microscopy (PALM). The use of photon-tolerant cell lines in combination with the high resolution and molecular sensitivity of PALM permitted us to investigate the nanoscale dynamics within individual adhesion complexes (ACs) in living cells under physiological conditions for as long as 25 min, with half of the time spent collecting the PALM images at spatial resolutions down to 60 nm and frame rates as short as 25 s. We visualized the formation of ...
	✔ Quantitative Colocalization Analysis of Multicolor Confocal Immunofluorescence Microscopy Images: Pushing Pixels to Explore Biological Phenomena [Quick Edit] [CiTO] Acta Histochemica et Cytochemica, Vol. 40, No. 4. (30 August 2007), pp. 101-111, doi:10.1267/ahc.07002 by Vadim Zinchuk, Olga Zinchuk, Teruhiko Okada posted by 5 people g-cianci mox guhjy plaissue kedmond Abstract Copy My Attachments My Copy Abstract Quantitative colocalization analysis is an advanced digital imaging tool to examine antigens of interest in immunofluorescence images obtained using confocal microscopes. It employs specialized algorithms to estimate the degree of overlap of fluorescence signals and thus enables acquiring important new information not otherwise obtainable using qualitative approaches alone. As raw confocal images have high levels of background, they should be prepared to become suitable for reliable calculation of colocalization coefficients by correcting it. We provide concise theoretical basis of quantitative colocalization ...
	✔ Fluorescence nanoscopy by ground-state depletion and single-molecule return [Quick Edit] [CiTO] Nature Methods, Vol. 5, No. 11. (15 September 2008), pp. 943-945, doi:10.1038/nmeth.1257 by Jonas Folling, Mariano Bossi, Hannes Bock, et al.Rebecca Medda, Christian A. Wurm, Birka Hein, Stefan Jakobs, Christian Eggeling, Stefan W. Hell posted by 7 people moernerlab djm3 bobturner plaissue samjlord alexknight marissalee Abstract Copy My Attachments My Copy Abstract We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein–labeled (living) samples, proving a simple yet powerful super-resolution approach. ...
	✔ Precise Nanometer Localization Analysis for Individual Fluorescent Probes [Quick Edit] [CiTO] Biophys J, Vol. 82, No. 5. (1 May 2002), pp. 2775-2783, doi:10.1016/s0006-3495(02)75618-x by Russell E. Thompson, Daniel R. Larson, Watt W. Webb posted by 12 people djm3 ARobson alexknight moernerlab bobturner sjysst baniel samjlord OliverHarriman mousearms plaissue melting Abstract Copy My Attachments My Copy Abstract Calculation of the centroid of the images of individual fluorescent particles and molecules allows localization and tracking in light microscopes to a precision about an order of magnitude greater than the microscope resolution. The factors that limit the precision of these techniques are examined and a simple equation derived that describes the precision of localization over a wide range of conditions. In addition, a localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm ...
	✔ Error bars in experimental biology [Quick Edit] [CiTO] The Journal of Cell Biology In J. Cell Biol., Vol. 177, No. 1. (09 April 2007), pp. 7-11, doi:10.1083/jcb.200611141 by Geoff Cumming, Fiona Fidler, David L. Vaux posted by 124 people lynx fxdm krokicki FlowCytometry abhishek_tiwari nahzef banso Posgrado en Ciencias Biomédicas fhsantanna Felipus_habilis srmblack flips mortenr guhjy structural_bioinformatics pcarbo cabbagezs Gorzomagnificent cbg wwweagle netzwerkerin Keksle natstreet amueller cdj39 silberbauer kristina williamrhenson awiedmer maniruzzaman inbetweener fredrikkarlsson emmahe Daria_G brian cisevol ardoi lgatto yangjustinc dullhunk fishtank rec3141 inesdesantiago Bioinformatics BergmanLab pjw2130 tt NIlz brianb davidmam vprieto yogeshpandit livingthingdan SCCS-iPS chrahn MingNi schoeband druvus gonzalez bmaust tny sen_cheng MHRashid dlabutin stalepig 217534 delenca ajaymalik Jporci lisa1 yangx82 VisionLab renatomilani mcc19732001 EvolSysBiol Microbial-Ecology manduca 2007lab yagyav cbonfil svenboekhoff takeshou arindrartow loopback007 kou_jinsei asadcbt amaru0867 jefferis idlegrraphx bangyuzhou cambray dbikard ana nelmor jdelcampo wilbur mjoach jfr tdmckee kuhn bayesian qwermish daforerog chriscole bpcusack jtcribbs aslupe maximilianh benoitvalley winterschlaefer ntakano Borelli Yanno jandam aleskladnik dayjm chad_davis devender rabu xiaoheilong batallah fuenfgeld rka2p lechristophe Abstract Copy My Attachments My Copy Abstract Error bars commonly appear in figures in publications, but experimental biologists are often unsure how they should be used and interpreted. In this article we illustrate some basic features of error bars and explain how they can help communicate data and assist correct interpretation. Error bars may show confidence intervals, standard errors, standard deviations, or other quantities. Different types of error bars give quite different information, and so figure legends must make clear what error bars represent. We suggest eight simple ...
	✔ How to Build a Motivated Research Group [Quick Edit] [CiTO] Mol Cell, Vol. 37, No. 2. (29 January 2010), pp. 151-152 by Uri Alon posted by 262 people dullhunk druvus songpku rossmounce Zephyrus azwinkau govindsimi rvosa leonardo rodney GuillaumeFilteau fungal djproctor timread herraiz VisionLab tarasdo VGreiff chenmengjie87 rebeccamancy jtcribbs geoforester matatk guillermina GustavoLacerda yyfwuhan cardiffuni edutars tnhh cerkut rodrigofaccioli vjekos Yanno jzm10nyc agy100 ama600 gvdh ianwelch ezm_mada JamesOrbinski Semantic-Social-Networks JeremyZucker ggailani logics of knowledge z3phyr jfrazee aaltenburger hayato1234 Zotero en español darian clairespahr mittinatten Torsten_Holmer Science of Teaching & Learning jenda_z istoyanov alun seduai alhoori 3127038 realfsen kees vprieto JoeVinegar kaniko rabelolincoln pesimov syah shaw6955 agong wangmimi kasianczuk Chang group schoeband kevinchannon mawds ajaymalik edws cambray cjfields alextrial ebojd ypjones migee emonroy cbg tuhinb plaissue starless afcallender R_C_Cordeiro JOcumpaugh Med Anthro @ UF smmy kevinemamy JoseBrox ihuston wsdou Publicase abellogin fsesser iman_tavassoly simonerfreitas ntsafnat weeks avilella Boxus jmchen1011 kvjanos CulCog Mnourian leegs ruvido burakeral TMichael pigironjoe daveGerrard mmuecke NIlz TeresitaAmezcua richardhwest sudarshaniisc netzwerkerin edwardbabkin zalabani hmedal spongelab christianharris replacement perforin CIDBH lijiejacy vitalyvchaban wmdeneve tejjyid pascalfrey maniruzzaman duplicates petrposik roryobrate ddahlem williamrhenson silberbauer xavig junghoo glynj nailest bertelsen surgeon167 gotrunc rettema Drrjhancock cwr ashko benoitvalley eumelus Diego_Prada maxweizhao mariopinedakrch andreassorge complex networks mblind megraw hmx _mkeller ks1250 spores svenboekhoff BOUKACEM cgleaniz lgevers jjray czarfv CFriedel skjq lmountr rodrigupo ErickOrtiz aky123 guhjy hairuo SCCS-iPS nbhammond MHRashid rybal AxelS jerry916 operon wwweagle cisevol ricardoerikson loison ThomasMichelsen iCeGS Thaverkamp kristina ingo cianan noamross viktor21614 INK-SSCI-SCI INK-SSCI-SCI@CiteuLike.org tmmurali uhale findroot tony_c PollardWall mordiano jalonsoarevalo drudmann miccoli runzi lwaldron cchang tiagoferreto fheintz nloose kozima bmoores di_hernandez xiaoheilong zzb3886 hectorcastro wahlstrom brunofed martydaniel ff FAB-lab Sal mc1975 thomaskreuz mschmuker atzayacatluspitufresas flbarroso kubyaddi phoenixzxl agyoung rcardoso malkocb NPress michaelbanks sasm csrocha joachimbaran Knowg-Mgt-CJCU theboyfree shangyung alexloh80 ddayjason A_Olympia hollace fbergmann applebyb jfr jketterer sby balabu UniQ mcc19732001 schlady BergmanLab Journal picks Abstract Copy My Attachments My Copy Abstract Motivated group members experience a full sense of choice: of doing what one wants. Such behavior shows high performance, is enjoyable, and enhances innovation. This essay describes principles of building a motivated research group. ...
	✔ Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time [Quick Edit] [CiTO] Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 5. (1 February 2005), pp. 1419-1423, doi:10.1073/pnas.0409487102 by L. Stirling Churchman, Zeynep Ökten, Ronald S. Rock, John F. Dawson, James A. Spudich posted by 9 people yunpeic mousearms plaissue alexknight quanwang moernerlab samjlord OliverHarriman melting Abstract Copy My Attachments My Copy Abstract Here we present a technique called single-molecule high-resolution colocalization (SHREC) of fluorescent dyes that allows the measurement of interfluorophore distances in macromolecules and macromolecular complexes with better than 10-nm resolution. By using two chromatically differing fluorescent molecules as probes, we are able to circumvent the Rayleigh criterion and measure distances much smaller than 250 nm. The probes are imaged separately and localized individually with high precision. The registration between the two imaging channels is measured by using fiduciary markers, and the ...
	✔ Image Analysis with Rapid and Accurate Two-Dimensional Gaussian Fitting [Quick Edit] [CiTO] Langmuir, Vol. 25, No. 14. (21 July 2009), pp. 8152-8160, doi:10.1021/la900393v by Stephen M. Anthony, Steve Granick posted by 4 people plaissue moernerlab samjlord kevinchannon Abstract Copy My Attachments My Copy Abstract PMID: 19419178 A computationally rapid image analysis method, weighted overdetermined regression, is presented for two-dimensional (2D) Gaussian fitting of particle location with subpixel resolution from a pixelized image of light intensity. Compared to least-squares Gaussian iterative fitting, which is most exact but prohibitively slow for large data sets, the precision of this new method is equivalent when the signal-to-noise ratio is high and approaches it when the signal-to-noise ratio is low, while enjoying a more than 100-fold improvement in computational time. ...
	✔ Communication in the Presence of Noise [Quick Edit] [CiTO] Proceedings of the IRE, Vol. 37, No. 1. (January 1949), pp. 10-21, doi:10.1109/jrproc.1949.232969 by C. E. Shannon posted by 12 people plaissue PierreRJ ejrrjs CarlosJ tictacgo jgrady75 douggeiger fredknabe moernerlab samjlord eyliu dcastro Abstract Copy My Attachments My Copy Abstract A method is developed for representing any communication system geometrically. Messages and the corresponding signals are points in two "function spaces," and the modulation process is a mapping of one space into the other. Using this representation, a number of results in communication theory are deduced concerning expansion and compression of bandwidth and the threshold effect. Formulas are found for the maxmum rate of transmission of binary digits over a system when the signal is perturbed by various types of noise. ...
	✔ Subdiffraction resolution in continuous samples [Quick Edit] [CiTO] Nature Photonics, Vol. 3, No. 7. (01 July 2009), pp. 362-364, doi:10.1038/nphoton.2009.102 by Rainer Heintzmann, Mats G. L. Gustafsson posted by 9 people meriemelkaroui plaissue eyliu Sese schmidtm pick600 moernerlab samjlord holx Abstract Copy My Attachments My Copy Abstract Super-resolution light microscopy methods either localize single molecular labels or treat the sample as a continuous object. The fundamental requirements for super-resolution in the continuum regime are spatially non-uniform illumination and a nonlinear photoresponse. ...
	✔ How to write consistently boring scientific literature [Quick Edit] [CiTO] Oikos, Vol. 116, No. 5. (May 2007), pp. 723-727, doi:10.1111/j.2007.0030-1299.15674.x by Kaj Sand-Jensen posted by 539 people LaurentPerrinet yochju fgb rhafner amerci janpaniev guhjy sspela Integrated Natural Resources Modelling and Management (INRMM) mtv goncalojustino brainpoolkurs rwoodhouse st3vil woodkn43 pratap lfriedl fierykylin cbonfil jdiezperezj pierredv fand9561 kristina JoseBrox derchao wuchangsong mz tminze pullus Semantic-Social-Networks hearton965 Anita13 tarokiritani syah fungal jornb87 nailest schobbes annampage Minimalist__ robsonbrown Enactment Neuroscience boosda cjeans brian dullhunk druvus brownstudy McCammon ckai1 tomhebbron proportional mittinatten ZhangXiatian ELMlab nuin ddimitriu pajoma gena mojin dynaopto phildeley mcc19732001 Rohdium kwoodsend timread idlegrraphx ucbcjbm mcartwri GuillaumeFilteau kevinemamy aidiss daichi_saito merlinnz antonijapetricusic jodeleeuw TVB a_u_petroff sensesublime rebeccamancy williamdwalker rickl dbk pawelsobko michaelbanks pirapira jrfine421 herrk ihuston pcalado leepro sebpaquet joawe96 maryamsa Usernamed BOUKACEM ulrix fergus rosenbla lmountr amoreno barry iskanbasal zhangrenxia janderz8 azwinkau czarfv buchgeher thorium klumsden agyoung librarymistress jcb1973 mrt2k9 OrganizationResearch konstantinos vengarre pick600 jgebbie c_hughes sgeuter ogerard jaia pipbird tkf MurphysLab jstenman cgleaniz Faerk ibuch orca allysonlister rohanlowe mjoach ombamawu operon CulCog rabio GustavoLacerda radoveden TKAB ommachi lq408 RobertSOakes henlimbai kpapachr kennysmith vabresp nellygidas wernien yezhenyu williamrhenson lenae simonalpha replacement robertporsch skjq christianharris rvasa aky123 spongelab dna tux2000 flydream0428 EneidaH Chrismartin76 tbastian jaswal4 akreh cmunson jmzhuo claudiushauptmann sachingarg jimo75 nellapower DavidG67 welliegirl kimbatchelor gvdh jaspervoskuilen fjanoos andre7520 richardmcgee coherent lperg Social Web arghavani debeerjonathan Geknitics nathancooper isp mykanth123 INK-SSCI-SCI scorullon nilda1ma loopback007 paas installe cm1acb clopes2010 fxdm lgedraite arthurdev telma_lopes renatazarate rajeshcsa cbniles randomname suizan ascharle sjones awooga ahaeusler ArtemPankin natstreet conradlee anphony Mohan-S soniapereira Dannycoutinho Horduna fredericraymond yeminjiao wkretzsch thiagomanel tverwaes mrvaidya Demeter CIIR michaelbreuker jeanwilson UAB Human Behavioral Neuroscience mfrichar johnmo mrsrikanth kdl kaniko pseudopharm scpeters ultrascichick VGreiff cisevol jjray charoy mmuecke bertelsen birte signori tnhh gwallau maglio polivares mdwelsh231 girabbit kaiserm thenose amadeusstevenson lanmuzi aldra SantoshKalwar pauljhurtado kieranhood cgravlee leonardo letillett livingthingdan UWA Science Communication Torsten_Holmer eudominguezmartin michaelfieseler jchiovaro marcela willwade teichman fisnik WeiquanXu wangxinxi kellychristoffersen rcardoso shivakmr netzwerkerin OPIG mcartright Laurentjp madderrabbit atlantis2360 ajaymalik mcastellani doc_solitude dejang jod999 Scholarly Communication knowlengr ntsafnat pbellec leliavski Wikipedia rudalert gareth rdonat alexandreconde fbaroni zzh1986 Layla-Renata simonerfreitas filipmarcinowski mschmuker sobolevnrm Leho DGiles atzayacatluspitufresas s-t-ahi TumSEC VisionLab applebyb fsesser prabhathanarajan freddemasi lxm1117 sebk hlbiotech magodehospital markeff BobWeiner tictacgo yusmi andreassorge mefrox beperron JOcumpaugh lhuarotop davidmackinnon stsaft aldamassinga nico_trompiste akid rsalmin bangyuzhou jasonn klazar JosephDavidson amhr jasonwalsh eduardoalvarez7 antonio-pgarcia amyfyn plaissue tuhinb chburrus banso mmansari dersebu nicolastripaldi annmcmahon trolles mzabidi chrisd arpaton makukha StephanMatthiesen arasbm mahlow dfdong mberger31 Librarians ehluis lynnefox ilyashl irishoconnor kaliczp Systematic Reviews Academic Development Group npotters aarre petrposik mcy scmelton zzb3886 wmdeneve spores noamross ypjones dutilh mahmoodr mariapage karimartins loison jennmaine calvin199 perbohner dalyx ararazul tuijajetsu Alessi mculbert chitsirin12 bmsousa rossmounce yetun guillermina zouchen621 jmchen1011 mfenner cs02 ErickOrtiz colmohara afcallender arthurkoestler hmedal sudarshaniisc LeilaTA asm666 jimeniux msbajammal dieabonnements starless ayolga galvareza10 istar TeresitaAmezcua klauso lyss bspackman robertlischke luciensamary CamilleH beete Fneesen metatheory Zephyrus nbawia zchen75 rabit NIlz AnthonySoprano pdpcosta eumelus jsatkosk wendygarnica jrw ansobol bhengeveld amynmoore christianholz poojamaj alhoori nmsx duel_jetty ulrichs simonhearne mmborges aaltenburger Sergey_gerbek lafuente sfalsharif Thaverkamp mthomure chriswillmott toszko cesardariovelandia cami mbromdhane gbierens kgrothoff brembs di_hernandez INK-SSCI-SCI@CiteuLike.org raf003 mzygmunt pcornill zio_tom78 tramullas alun hideojoho HCCSProgram Journal picks jimburton JillianHowlett orrajabi janneb clementinecornu amjedbj dgront ulmer sdaehne ibre timsenior pvdg Med Anthro @ UF lmichan sxs732 artaban421 akuhn hkimura jcreed R_C_Cordeiro nGrenier alexander_bauer Gaetan chris sniklas aryanshining mwd petko_bogdanov carelcad Tomste yangjustinc klexa tunheim Elley AJCann Publicase Thermaneutist Dori2008 flips arsyed sjefarrar abellogin psique jjrohal markusd mschmer BergmanLab toomash Borelli sterovetta maike echinotrix jford TaqSys soumitri GeeSharpMinor tumo erklaerbaer STS ColDyn feminist_technoscience Academic Writing, Literacies and Discourse hejibo Abstract Copy My Attachments My Copy Abstract Although scientists typically insist that their research is very exciting and adventurous when they talk to laymen and prospective students, the allure of this enthusiasm is too often lost in the predictable, stilted structure and language of their scientific publications. I present here, a top-10 list of recommendations for how to write consistently boring scientific publications. I then discuss why we should and how we could make these contributions more accessible and exciting. ...
	✔ Fiji: an open-source platform for biological-image analysis. [Quick Edit] [CiTO] Nature methods, Vol. 9, No. 7. (28 July 2012), pp. 676-682, doi:10.1038/nmeth.2019 by Johannes Schindelin, Ignacio Arganda-Carreras, Erwin Frise, et al.Verena Kaynig, Mark Longair, Tobias Pietzsch, Stephan Preibisch, Curtis Rueden, Stephan Saalfeld, Benjamin Schmid, Jean-Yves Y. Tinevez, Daniel James J. White, Volker Hartenstein, Kevin Eliceiri, Pavel Tomancak, Albert Cardona posted by 7 people polivares guhjy sgclendenon SoftlySplinter plaissue nickholway nsm120 Abstract Copy My Attachments My Copy Abstract Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis. Fiji uses modern software engineering practices to combine powerful software libraries with a broad range of scripting languages to enable rapid prototyping of image-processing algorithms. Fiji facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system. We propose Fiji as ...
	✔ ImageJ for microscopy. [Quick Edit] [CiTO] BioTechniques, Vol. 43, No. 1 Suppl. (July 2007), pp. 25-30 by Tony J. Collins posted by 3 people guhjy plaissue lechristophe Abstract Copy My Attachments My Copy Abstract ImageJ is an essential tool for us that fulfills most of our routine image processing and analysis requirements. The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget. ...
	✔ Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C [Quick Edit] [CiTO] Journal of Cell Science, Vol. 101, No. 2. (01 February 1992), pp. 415-425 by C. M. Anderson, G. N. Georgiou, I. E. Morrison, G. V. Stevenson, R. J. Cherry posted by 5 people dimiccolimariella rodney Kopelman_Group charsooghi plaissue Abstract Copy My Attachments My Copy Abstract A fluorescence imaging system, based on using a cooled slow-scan CCD camera, has been developed for tracking receptors on the surfaces of living cells. The technique is applicable to receptors for particles such as lipoproteins and viruses that can be labeled with a few tens of fluorophores. The positions of single particles in each image are determined to within 25 nm by fitting the fluorescence distribution to a two-dimensional Gaussian function. This procedure also provides an accurate measure of intensity, which ...
	✔ Quantitative comparison of algorithms for tracking single fluorescent particles. [Quick Edit] [CiTO] Biophysical journal, Vol. 81, No. 4. (1 October 2001), pp. 2378-2388, doi:10.1016/s0006-3495(01)75884-5 by M. K. Cheezum, W. F. Walker, W. H. Guilford posted by 5 people plaissue Kopelman_Group rodney kevinchannon MelissaMaczka Abstract Copy My Attachments My Copy Abstract Single particle tracking has seen numerous applications in biophysics, ranging from the diffusion of proteins in cell membranes to the movement of molecular motors. A plethora of computer algorithms have been developed to monitor the sub-pixel displacement of fluorescent objects between successive video frames, and some have been claimed to have "nanometer" resolution. To date, there has been no rigorous comparison of these algorithms under ...
	✔ Fluorescent protein FRET: the good, the bad and the ugly [Quick Edit] [CiTO] Trends in Biochemical Sciences, Vol. 32, No. 9. (September 2007), pp. 407-414, doi:10.1016/j.tibs.2007.08.003 by David W. Piston, Gert-Jan Kremers posted by 14 people dchughes kjell mousearms Vincent_Rouilly plaissue schmidtm haisent dark_innocent lechristophe G4ID neurolit Genetics-of-Gambling guhjy nahnah Abstract Copy My Attachments My Copy Abstract Dynamic protein interactions play a significant part in many cellular processes. A technique that shows considerable promise in elucidating such interactions is Förster resonance energy transfer (FRET). When combined with multiple, colored fluorescent proteins, FRET permits high spatial resolution assays of protein–protein interactions in living cells. Because FRET signals are usually small, however, their measurement requires careful interpretation and several control experiments. Nevertheless, the use of FRET in cell biological experiments has exploded over the past few years. Here we describe ...
	✔ Common tasks in microscopic and ultrastructural image analysis using ImageJ. [Quick Edit] [CiTO] Ultrastructural pathology, Vol. 31, No. 6. (c 2007), pp. 401-407, doi:10.1080/01913120701719189 by Francesca Papadopulos, Matthew Spinelli, Sabrina Valente, et al.Laura Foroni, Catia Orrico, Francesco Alviano, Gianandrea Pasquinelli posted by 2 people guhjy plaissue Abstract Copy My Attachments My Copy Abstract Cooperation between research communities and software-development teams has led to the creation of novel software. The purpose of this paper is to show an alternative work method based on the usage of ImageJ (http://rsb.info.nih.gov/ij/), which can be effectively employed in solving common microscopic and ultrastructural image analysis tasks. As an open-source software, ImageJ provides the possibility to work in a free-development/sharing world. Its very "friendly" graphical user interface helps users to manage and edit biomedical images. The on-line material such as ...
	✔ A high-level 3D visualization API for Java and ImageJ. [Quick Edit] [CiTO] BMC bioinformatics, Vol. 11, No. 1. (2010), 274, doi:10.1186/1471-2105-11-274 by Benjamin Schmid, Johannes Schindelin, Albert Cardona, Mark Longair, Martin Heisenberg posted by 5 people erexhepa cudmore cmor guhjy plaissue Abstract Copy My Attachments My Copy Abstract Current imaging methods such as Magnetic Resonance Imaging (MRI), Confocal microscopy, Electron Microscopy (EM) or Selective Plane Illumination Microscopy (SPIM) yield three-dimensional (3D) data sets in need of appropriate computational methods for their analysis. The reconstruction, segmentation and registration are best approached from the 3D representation of the data set. ...
	✔ Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting [Quick Edit] [CiTO] Science, Vol. 306, No. 5700. (19 November 2004), pp. 1370-1373, doi:10.1126/science.1102506 by Ryoko Ando, Hideaki Mizuno, Atsushi Miyawaki posted by 3 people inbetweener samjlord moernerlab Abstract Copy My Attachments My Copy Abstract The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, ...
	✔ Computational imaging in cell biology. [Quick Edit] [CiTO] The Journal of cell biology, Vol. 161, No. 3. (12 May 2003), pp. 477-481, doi:10.1083/jcb.200302097 by Roland Eils, Chaitanya Athale posted by 5 people plaissue Posgrado en Ciencias Biomédicas fercisob singhsh Tumor Microenvironment @ OSU Abstract Copy My Attachments My Copy Abstract Microscopy of cells has changed dramatically since its early days in the mid-seventeenth century. Image analysis has concurrently evolved from measurements of hand drawings and still photographs to computational methods that (semi-) automatically quantify objects, distances, concentrations, and velocities of cells and subcellular structures. Today's imaging technologies generate a wealth of data that requires visualization and multi-dimensional and quantitative image analysis as prerequisites to turning ...
	✔ The fence and picket structure of the plasma membrane of live cells as revealed by single molecule techniques (Review). [Quick Edit] [CiTO] Molecular membrane biology, Vol. 20, No. 1. (r 2003), pp. 13-18 by Ken Ritchie, Ryoto Iino, Takahiro Fujiwara, Kotono Murase, Akihiro Kusumi posted by 2 people plaissue madhadron Abstract Copy My Attachments My Copy Abstract Models of the organization of the plasma membrane of live cells as discovered through diffusion measurements of integral membrane molecules (transmembrane and GPI-anchored proteins, and lipid) at the single molecule level are discussed. Diffusion of transmembrane protein and, indeed, even lipid is anomalous in that the molecules tend to diffuse freely in limited size compartments, with infrequent intercompartment transitions. This average residency time in a ...
	✔ Surfing on a new wave of single-molecule fluorescence methods [Quick Edit] [CiTO] Physical Biology, Vol. 7, No. 3. (04 August 2010), 031001, doi:10.1088/1478-3975/7/3/031001 by Johannes Hohlbein, Kristofer Gryte, Mike Heilemann, Achillefs N. Kapanidis posted by 4 people russell_aidan samjlord plaissue moernerlab Abstract Copy My Attachments My Copy Abstract Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are characterized by a substantial increase in complexity of the instrumentation or biological samples involved. Specifically, we describe new multi-laser and multi-colour fluorescence spectroscopy and imaging techniques, super-resolution microscopy imaging and the development of instruments that combine fluorescence detection with other single-molecule methods such as force spectroscopy. We also highlight two pivotal ...
	✔ Superresolution Imaging using Single-Molecule Localization [Quick Edit] [CiTO] Annual Review of Physical Chemistry, Vol. 61, No. 1. (4 January 2010), pp. 345-367, doi:10.1146/annurev.physchem.012809.103444 by George Patterson, Michael Davidson, Suliana Manley, Jennifer L. Schwartz posted by 4 people ts3435 samjlord plaissue moernerlab Abstract Copy My Attachments My Copy Abstract Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (∼10–20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule-based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable ...
	✔ Real-time computation of subdiffraction-resolution fluorescence images [Quick Edit] [CiTO] Journal of Microscopy, Vol. 237, No. 1. (January 2010), pp. 12-22, doi:10.1111/j.1365-2818.2009.03287.x by S. Wolter, M. Schüttpelz, M. Tscherepanow, S. Van de Linde, M. Heilemann, M. Sauer posted by 4 people plaissue mousearms moernerlab samjlord Abstract Copy My Attachments My Copy Abstract Summary In the recent past, single-molecule based localization or photoswitching microscopy methods such as stochastic optical reconstruction microscopy (STORM) or photoactivated localization microscopy (PALM) have been successfully implemented for subdiffraction-resolution fluorescence imaging. However, the computational effort needed to localize numerous fluorophores is tremendous, causing long data processing times and thereby limiting the applicability of the technique. Here we present a new computational scheme for data processing consisting of noise reduction, detection of likely fluorophore positions, high-precision fluorophore localization and subsequent visualization ...
	✔ Handbook of Biological Confocal Microscopy [Quick Edit] [CiTO] (02 June 2006) posted by 8 people plaissue NicMag singhsh amcmorl cherrie pscopelliti Tumor Microenvironment @ OSU weeks Abstract Copy My Attachments My Copy Abstract This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. ...
	✔ Illuminating the life of GPCRs. [Quick Edit] [CiTO] Cell communication and signaling : CCS, Vol. 7, No. 1. (2009), 16, doi:10.1186/1478-811x-7-16 by Ilka Böhme, Annette G. Beck-Sickinger posted by 3 people plaissue guhjy viktor21614 Abstract Copy My Attachments My Copy Abstract ABSTRACT: The investigation of biological systems highly depends on the possibilities that allow scientists to visualize and quantify biomolecules and their related activities in real-time and non-invasively. G-protein coupled receptors represent a family of very dynamic and highly regulated transmembrane proteins that are involved in various important physiological processes. Since their localization is not confined to the cell surface they have been a very attractive "moving target" and the understanding of their intracellular pathways as well as the identified protein-protein-interactions has ...
	✔ Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae. [Quick Edit] [CiTO] The Journal of cell biology, Vol. 135, No. 6 Pt 1. (1 December 1996), pp. 1535-1549, doi:10.1083/jcb.135.6.1535 by K. L. Hill, N. L. Catlett, L. S. Weisman posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract During cell division, cytoplasmic organelles are not synthesized de novo, rather they are replicated and partitioned between daughter cells. Partitioning of the vacuole in the budding yeast Saccharomyces cerevisiae is coordinated with the cell cycle and involves a dramatic translocation of a portion of the parental organelle from the mother cell into the bud. While the molecular mechanisms that mediate this event are unknown, the vacuole's rapid and directed movements suggest cytoskeleton involvement. To identify cytoskeletal components that function in this ...
	✔ Optimising the precision for localising fluorescent proteins in living cells by 2D Gaussian fitting of digital images: application to COPII-coated endoplasmic reticulum exit sites. [Quick Edit] [CiTO] European biophysics journal : EBJ, Vol. 37, No. 8. (27 October 2008), pp. 1335-1349, doi:10.1007/s00249-008-0343-7 by Peter Spence, Vijay Gupta, David J. Stephens, Andrew J. Hudson posted by 2 people lechristophe plaissue Abstract Copy My Attachments My Copy Abstract An insight into the operation of molecular motors has already been obtained under in vitro conditions from single-molecule tracking of proteins. It remains to analyse the effects of these motors on the position and secretion of specific organelles in the environment of the cell. For this purpose, we have investigated the accuracy of a standard algorithm to enable the tracking of particles in live-cell microscopy. The results have been applied to an example study into the role of the microtubule-motor kinesin ...
	✔ Lipid Rafts As a Membrane-Organizing Principle [Quick Edit] [CiTO] Science, Vol. 327, No. 5961. (01 January 2010), pp. 46-50, doi:10.1126/science.1174621 by Daniel Lingwood, Kai Simons posted by 14 people Oxford Virology chiufanlee paulschlesinger bingcheng moernerlab dsmueller plaissue fehabesha TDF agrossfield guhjy samjlord mc1975 wsjames Abstract Copy My Attachments My Copy Abstract Cell membranes display a tremendous complexity of lipids and proteins designed to perform the functions cells require. To coordinate these functions, the membrane is able to laterally segregate its constituents. This capability is based on dynamic liquid-liquid immiscibility and underlies the raft concept of membrane subcompartmentalization. Lipid rafts are fluctuating nanoscale assemblies of sphingolipid, cholesterol, and proteins that can be stabilized to coalesce, forming platforms ...
	✔ Super-resolution optical microscopy: multiple choices [Quick Edit] [CiTO] Current Opinion in Chemical Biology, Vol. 14, No. 1. (10 February 2010), pp. 10-14, doi:10.1016/j.cbpa.2009.10.013 by Bo Huang posted by 4 people bobturner plaissue moernerlab samjlord Abstract Copy My Attachments My Copy Abstract The recent invention of super-resolution optical microscopy enables the visualization of fine features in biological samples with unprecedented clarity. It creates numerous opportunities in biology because vast amount of previously obscured subcellular processes now can be directly observed. Rapid development in this field in the past two years offers many imaging modalities that address different needs but they also complicates the choice of the ‘perfect’ method for answering a specific question. Here I will briefly describe the principles of super-resolution optical ...
	✔ Targeted Photoswitchable Probe for Nanoscopy of Biological Structures [Quick Edit] [CiTO] ChemBioChem, Vol. 11, No. 10. (10 June 2010), pp. 1361-1363, doi:10.1002/cbic.201000189 by Claudio Dellagiacoma, Gražvydas Lukinavičius, Noelia Bocchio, et al.Sambashiva Banala, Stefan Geissbühler, Iwan Märki, Kai Johnsson, Theo Lasser posted by 5 people djm3 Diels-Alder01 plaissue moernerlab samjlord Copy My Attachments My Copy
	✔ NIH Image to ImageJ: 25 years of image analysis [Quick Edit] [CiTO] Nature Methods, Vol. 9, No. 7. (28 June 2012), pp. 671-675, doi:10.1038/nmeth.2089 by Caroline A. Schneider, Wayne S. Rasband, Kevin W. Eliceiri posted by 5 people polivares plaissue nickholway Helium Ion Microscopy lawa Copy My Attachments My Copy
	✔ Coordinate-based colocalization analysis of single-molecule localization microscopy data. [Quick Edit] [CiTO] Histochemistry and cell biology, Vol. 137, No. 1. (1 January 2012), pp. 1-10, doi:10.1007/s00418-011-0880-5 by Sebastian Malkusch, Ulrike Endesfelder, Justine Mondry, Márton Gelléri, Peter J. Verveer, Mike Heilemann posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract Colocalization of differently labeled biomolecules is a valuable tool in fluorescence microscopy and can provide information on biomolecular interactions. With the advent of super-resolution microscopy, colocalization analysis is getting closer to molecular resolution, bridging the gap to other technologies such as fluorescence resonance energy transfer. Among these novel microscopic techniques, single-molecule localization-based super-resolution methods offer the advantage of providing single-molecule coordinates that, rather than intensity ...
	✔ Image acquisition for colocalization using optical microscopy [Quick Edit] [CiTO] Am J Physiol Cell Physiol, Vol. 294, No. 5. (1 May 2008), pp. C1119-1122, doi:10.1152/ajpcell.00133.2008 by David R. L. Scriven, Ronald M. Lynch, Edwin D. W. Moore posted by 2 people guhjy plaissue Abstract Copy My Attachments My Copy Abstract Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common analytical tool in cell biology. Interpreting the images and the meaning of this identified coincidence is difficult in the absence of basic information about the acquisition parameters. In this commentary, we highlight important factors in the acquisition of images used to demonstrate colocalization, and we discuss the minimum information that authors should include in a manuscript so ...
	✔ Colocalization analysis yields superior results after image restoration. [Quick Edit] [CiTO] Microscopy research and technique, Vol. 64, No. 2. (1 June 2004), pp. 103-112, doi:10.1002/jemt.20066 by Lukas Landmann, Permsin Marbet posted by 2 people guhjy plaissue Abstract Copy My Attachments My Copy Abstract Colocalization analysis is a powerful tool for the demonstration of spatial and temporal overlap in the distribution patterns of fluorescent probes. In unprocessed images, background affects image quality by impairing resolution and obscuring image detail in the low-intensity range. Because confocal images suffer from background levels up to 30% maximum intensity, colocalization analysis, which is a typical segmentation process, is limited to high-intensity signal. In ...
	✔ Quantifying colocalization by correlation: the Pearson correlation coefficient is superior to the Mander's overlap coefficient. [Quick Edit] [CiTO] Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 77, No. 8. (August 2010), pp. 733-742, doi:10.1002/cyto.a.20896 by Jeremy Adler, Ingela Parmryd posted by 2 people guhjy plaissue Abstract Copy My Attachments My Copy Abstract The Pearson correlation coefficient (PCC) and the Mander's overlap coefficient (MOC) are used to quantify the degree of colocalization between fluorophores. The MOC was introduced to overcome perceived problems with the PCC. The two coefficients are mathematically similar, differing in the use of either the absolute intensities (MOC) or of the deviation from the mean (PCC). A range of correlated datasets, which extend to the ...
	✔ Retinitis pigmentosa. [Quick Edit] [CiTO] Lancet, Vol. 368, No. 9549. (18 November 2006), pp. 1795-1809, doi:10.1016/s0140-6736(06)69740-7 by Dyonne T. Hartong, Eliot L. Berson, Thaddeus P. Dryja posted by 6 people silva45 Yanno adiazar yagyav zahir85 inbetweener Abstract Copy My Attachments My Copy Abstract Hereditary degenerations of the human retina are genetically heterogeneous, with well over 100 genes implicated so far. This Seminar focuses on the subset of diseases called retinitis pigmentosa, in which patients typically lose night vision in adolescence, side vision in young adulthood, and central vision in later life because of progressive loss of rod and cone photoreceptor cells. Measures of retinal function, such as the electroretinogram, show that photoreceptor function is diminished generally many years before symptomic night blindness, visual-field scotomas, ...
	✔ Visualization of Fermi's Golden Rule Through Imaging of Light Emission from Atomic Silver Chains [Quick Edit] [CiTO] Science, Vol. 325, No. 5943. (21 August 2009), pp. 981-985, doi:10.1126/science.1174592 by Chi Chen, C. A. Bobisch, W. Ho posted by 5 people andreassorge plaissue pem moernerlab samjlord Abstract Copy My Attachments My Copy Abstract Atomic-scale spatial imaging of one-dimensional chains of silver atoms allows Fermi's golden rule, a fundamental principle governing optical transitions, to be visualized. We used a scanning tunneling microscope (STM) to assemble a silver atom chain on a nickel-aluminum alloy surface. Photon emission was induced with electrons from the tip of the STM. The emission was spatially resolved with subnanometer resolution by changing the tip position along the chain. The number and positions of the emission maxima in the photon images match ...
	✔ A Framework for Detailed Objective Comparison of Non-rigid Registration Algorithms in Neuroimaging [Quick Edit] [CiTO] In Medical Image Computing and Computer-Assisted Intervention – MICCAI 2004, Vol. 3216 (2004), pp. 679-686, doi:10.1007/978-3-540-30135-6_83 by William R. Crum, Daniel Rueckert, Mark Jenkinson, David Kennedy, Stephen M. Smith edited by Christian Barillot, David R. Haynor, Pierre Hellier posted by 2 people plaissue ben500 Abstract Copy My Attachments My Copy Abstract Non-rigid image registration is widely used in the analysis of brain images to the extent it is provided as a standard tool in common packages such as SPM. However the performance of algorithms in specific applications remains hard to measure. In this paper a detailed comparison of the performance of an affine, B-Spline control-point and viscous fluid registration algorithm in inter-subject brain registration is presented. The comparison makes use of highly detailed expert manual labellings of a range of structures distributed ...
	✔ Neuropathological basis of magnetic resonance images in aging and dementia [Quick Edit] [CiTO] Annals of Neurology, Vol. 63, No. 1. (January 2008), pp. 72-80, doi:10.1002/ana.21296 by William J. Jagust, Ling Zheng, Danielle J. Harvey, et al.Wendy J. Mack, Harry V. Vinters, Michael W. Weiner, William G. Ellis, Chris Zarow, Dan Mungas, Bruce R. Reed, Joel H. Kramer, Norbert Schuff, Charles DeCarli, Helena C. Chui posted by 2 people mfirbank plaissue Abstract Copy My Attachments My Copy Abstract Magnetic resonance (MR) imaging is used widely for assessment of patients with cognitive impairment, but the pathological correlates are unclear, especially when multiple pathologies are present.This report includes 93 subjects from a longitudinally followed cohort recruited for the study of Alzheimer's disease (AD) and subcortical cerebrovascular disease (CVD). MR images were analyzed to quantify cortical gray matter volume, hippocampal volume, white matter hyperintensities, and lacunes. Neuropathological examination quantified CVD parenchymal pathology, AD pathology (defined as Consortium to Establish a Registry for ...
	✔ A comparative analysis of structural brain MRI in the diagnosis of Alzheimer's disease. [Quick Edit] [CiTO] Behavioural neurology, Vol. 21, No. 1. (2009), pp. 13-19, doi:10.3233/ben-2009-0225 by Jason Appel, Elizabeth Potter, Qian Shen, et al.Gustavo Pantol, Maria T. Greig, David Loewenstein, Ranjan Duara posted by 2 people srmblack plaissue Abstract Copy My Attachments My Copy Abstract Dementia is a debilitating and life-altering disease which leads to both memory impairment and decline of normal executive functioning. While causes of dementia are numerous and varied, the leading cause among patients 60 years and older is Alzheimer's disease. The gold standard for Alzheimer's diagnosis remains histological identification of amyloid plaques and neurofibrillary tangles within the medial temporal lobe, more specifically the entorhinal cortex and hippocampus. Although no definitive cure for Alzheimer's disease currently exists, there are treatments targeted at preserving ...
	✔ The physical chemistry of membrane curvature [Quick Edit] [CiTO] Nature Chemical Biology, Vol. 5, No. 11. (01 November 2009), pp. 783-784, doi:10.1038/nchembio.247 by Jay T. Groves posted by 5 people NE_baer plaissue paulschlesinger moernerlab samjlord Abstract Copy My Attachments My Copy Abstract Membrane curvature sensing by amphipathic helices is an emergent property of the ensemble of molecules and membrane sites. New data suggest that individual molecules do not experience stronger binding to curved membranes. Controlling the spatial arrangement of their constituent molecules is a defining characteristic of living organisms. Among the most fundamental of life's organizational motifs is the lipid bilayer membrane, and regulation of membrane curvature is central to innumerable biochemical processes1, 2. ...
	✔ Temperature Stress Causes Host Cell Detachment in Symbiotic Cnidarians: Implications for Coral Bleaching [Quick Edit] [CiTO] Biol Bull, Vol. 182, No. 3. (1 June 1992), pp. 324-332 by R. D. Gates, G. Baghdasarian, L. Muscatine posted by 2 people bgranger plaissue Copy My Attachments My Copy
	✔ Extraction of spots in biological images using multiscale products [Quick Edit] [CiTO] pp. 1989-1996 by J. C. Olivo-Marin posted by 2 people plaissue mkuzak Abstract Copy My Attachments My Copy Abstract We present a new method to detect and count bright spots in fluorescence images coming from biological immunomicroscopy experiments. It is based on the multiscale product of subband images resulting from the a trous wavelet transform decomposition of the original image, after thresholding of non-significant coefficients. The multiscale correlation of the filtered wavelet coefficients, which allows to enhance multiscale peaks due to spots while reducing noise, combines information coming from different levels of resolution and gives a clear and distinctive chacterization ...
	✔ Multimodal surface-based morphometry reveals diffuse cortical atrophy in traumatic brain injury. [Quick Edit] [CiTO] BMC medical imaging, Vol. 9 (31 December 2009), 20, doi:10.1186/1471-2342-9-20 by And U. Turken, Timothy J. Herron, Xiaojian Kang, et al.Larry E. O'Connor, Donna J. Sorenson, Juliana V. Baldo, David L. Woods posted by 2 people rcr1991 plaissue Abstract Copy My Attachments My Copy Abstract BACKGROUND: Patients with traumatic brain injury (TBI) often present with significant cognitive deficits without corresponding evidence of cortical damage on neuroradiological examinations. One explanation for this puzzling observation is that the diffuse cortical abnormalities that characterize TBI are difficult to detect with standard imaging procedures. Here we investigated a patient with severe TBI-related cognitive impairments whose scan was interpreted as normal by a board-certified radiologist in order to determine if quantitative neuroimaging could detect cortical abnormalities not evident with standard neuroimaging ...
	✔ Early diagnosis of Alzheimer's disease: contribution of structural neuroimaging. [Quick Edit] [CiTO] NeuroImage, Vol. 18, No. 2. (February 2003), pp. 525-541 by Gaël Chetelat, Jean-Claude C. Baron posted by 3 people plaissue soundray kamilvlcek Abstract Copy My Attachments My Copy Abstract To accurately predict the development of Alzheimer's disease (AD) at its predementia stage would be a major breakthrough from both therapeutic and research standpoints. In this review, our focus is on markers obtained with structural imaging--especially magnetic resonance imaging (MRI)--and on studies of subjects at risk of developing AD. Among the latter, amnestic mild cognitive impairment (MCI) is currently the most commonly accepted reference, and therefore is specially targeted in this review. MCI refers to patients with significant but isolated memory ...
	✔ Polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion. [Quick Edit] [CiTO] Molecular biology of the cell, Vol. 21, No. 10. (15 May 2010), pp. 1737-1752, doi:10.1091/mbc.e09-08-0706 by Dmitry V. Suchkov, Reagan DeFlorio, Edward Draper, et al.Amber Ismael, Madhushalini Sukumar, Robert Arkowitz, David E. Stone posted by 3 people m_j plaissue Keksle Abstract Copy My Attachments My Copy Abstract In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone induces its polarization into "crescents" that cap the future mating projection. Here, we find that in pheromone-treated cells, receptor crescents are visible before detectable polarization of actin cables and that the receptor can polarize in the absence of actin-dependent directed ...
	✔ Tracking single particles: a user-friendly quantitative evaluation [Quick Edit] [CiTO] Physical Biology, Vol. 2, No. 1. (01 March 2005), 60, doi:10.1088/1478-3967/2/1/008 by Brian C. Carter, George T. Shubeita, Steven P. Gross posted by 2 people plaissue kvdesai Abstract Copy My Attachments My Copy Abstract As our knowledge of biological processes advances, we are increasingly aware that cells actively position sub-cellular organelles and other constituents to control a wide range of biological processes. Many studies quantify the position and motion of, for example, fluorescently labeled proteins, protein aggregates, mRNA particles or virus particles. Both differential interference contrast (DIC) and fluorescence microscopy can visualize vesicles, nuclei or other small organelles moving inside cells. While such studies are increasingly important, there has been no complete analysis of the ...
	✔ Observations of the tissue-skeleton interface in the scleractinian coral Stylophora pistillata [Quick Edit] [CiTO] Coral Reefs In Coral Reefs, Vol. 26, No. 3. (1 September 2007), pp. 517-529, doi:10.1007/s00338-007-0263-5 by E. Tambutté, D. Allemand, D. Zoccola, et al.A. Meibom, S. Lotto, N. Caminiti, S. Tambutté posted by 3 people ericcsm Maysoon plaissue Abstract Copy My Attachments My Copy Abstract Recent micro-analytical studies of coral skeletons have led to the discovery that the effects of biology on the skeletal chemical and isotopic composition are not uniform over the skeleton. The aim of the present work was to provide histological observations of the coral tissue at the interface with the skeleton, using Stylophora pistillata as a model, and to discuss these observations in the context of skeletal ultra-structural organization and composition. Several important observations are reported: (1) At all scales of observation, ...
	✔ 3D deconvolution microscopy. [Quick Edit] [CiTO] Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] In Current Protocols in Cytometry, Vol. Chapter 12 (April 2010), doi:10.1002/0471142956.cy1219s52 by David S. Biggs posted by 2 people plaissue abrazhe Abstract Copy My Attachments My Copy Abstract 3D deconvolution microscopy is a combination of optical and computational techniques that are used to maximize the observed resolution and signal from a biological specimen. Mathematical models are used to predict the distribution of out-of-focus light caused by the inherent optical limitations of the instrument, which can then be compensated for using computer algorithms. This unit will review the theory of image formation and characteristics ...
	✔ Candida albicans hyphae have a Spitzenkörper that is distinct from the polarisome found in yeast and pseudohyphae [Quick Edit] [CiTO] Journal of Cell Science, Vol. 118, No. 13. (01 July 2005), pp. 2935-2947, doi:10.1242/jcs.02414 by Helen Crampin, Kenneth Finley, Maryam Gerami-Nejad, et al.Helen Court, Cheryl Gale, Judith Berman, Peter Sudbery posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract Fungi grow with a variety of morphologies: oval yeast cells, chains of elongated cells called pseudohyphae and long, narrow, tube-like filaments called hyphae. In filamentous fungi, hyphal growth is strongly polarised to the tip and is mediated by the Spitzenkörper, which acts as a supply centre to concentrate the delivery of secretory vesicles to the tip. In the budding yeast Saccharomyces cerevisiae, polarised growth is mediated by the polarisome, a surface cap of proteins that nucleates the formation of actin cables ...
	✔ Adaptation of core mechanisms to generate cell polarity. [Quick Edit] [CiTO] Nature, Vol. 422, No. 6933. (17 April 2003), pp. 766-774, doi:10.1038/nature01602 by W. James Nelson posted by 4 people plaissue wei spongelab Keksle Abstract Copy My Attachments My Copy Abstract Cell polarity is defined as asymmetry in cell shape, protein distributions and cell functions. It is characteristic of single-cell organisms, including yeast and bacteria, and cells in tissues of multi-cell organisms such as epithelia in worms, flies and mammals. This diversity raises several questions: do different cell types use different mechanisms to generate polarity, how is polarity signalled, how do cells react to that signal, and how is structural polarity translated into specialized functions? Analysis of evolutionarily diverse cell types reveals ...
	✔ Ustilago maydis, a new fungal model system for cell biology. [Quick Edit] [CiTO] Trends in cell biology, Vol. 18, No. 2. (1 February 2008), pp. 61-67, doi:10.1016/j.tcb.2007.11.008 by Gero Steinberg, Jose Perez-Martin posted by 4 people plaissue jecastillo stajich Mycology Abstract Copy My Attachments My Copy Abstract The use of fungal model systems, such as Saccharomyces cerevisisae and Schizosaccharomyces pombe, has contributed enormously to our understanding of essential cellular processes in animals. Here, we introduce the corn smut fungus Ustilago maydis as a new model organism for studying cell biological processes. Genome-wide analysis demonstrates that U. maydis is more closely related to humans than to budding yeast, and numerous proteins are shared ...
	✔ The role of image registration in brain mapping. [Quick Edit] [CiTO] Image and vision computing, Vol. 19, No. 1-2. (1 January 2001), pp. 3-24, doi:10.1016/s0262-8856(00)00055-x by A. W. Toga, P. M. Thompson posted by 2 people rv101 plaissue Abstract Copy My Attachments My Copy Abstract Image registration is a key step in a great variety of biomedical imaging applications. It provides the ability to geometrically align one dataset with another, and is a prerequisite for all imaging applications that compare datasets across subjects, imaging modalities, or across time. Registration algorithms also enable the pooling and comparison of experimental findings across laboratories, the construction of population-based brain atlases, and the creation ...
	✔ Homologues of yeast polarity genes control the development of multinucleated hyphae in Ashbya gossypii. [Quick Edit] [CiTO] Current opinion in microbiology, Vol. 8, No. 4. (August 2005), pp. 370-377, doi:10.1016/j.mib.2005.06.021 by Peter Philippsen, Andreas Kaufmann, Hans-Peter P. Schmitz posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract A few years ago, A. gossypii became recognized as an attractive model to study the growth of long and multinucleated fungal cells (hyphae) because of its small genome, haploid nuclei, and efficient gene targeting methods. It is generally assumed that a better understanding of filamentous fungal growth will greatly stimulate the development of novel fungicides. The use of Ashbya gossypii as a model is particularly promising because of the high level of gene order conservation (synteny) between the genomes of A. ...
	✔ Study of calcification during a daily cycle of the coral Stylophora pistillata: implications for 'light-enhanced calcification'. [Quick Edit] [CiTO] The Journal of experimental biology, Vol. 209, No. Pt 17. (September 2006), pp. 3413-3419, doi:10.1242/jeb.02382 by Aurélie Moya, Sylvie Tambutté, Eric Tambutté, Didier Zoccola, Natacha Caminiti, Denis Allemand posted by 2 people ericcsm plaissue Abstract Copy My Attachments My Copy Abstract This work, performed on the scleractinian coral Stylophora pistillata, aims at providing new information on the 'light-enhanced calcification' process. In a first step, in controlled conditions of culture and constant light supply, we studied the diurnal cycle of calcification. We determined that calcification rates are constant during the day and the night with a 2.6-fold difference between day and night calcification rates. We also showed that the photosynthetic rate is constant throughout the day when a constant light intensity is applied. ...
	✔ Coral Calcification, Cells to Reefs Coral Reefs: An Ecosystem in Transition [Quick Edit] [CiTO] In Coral Reefs: An Ecosystem in Transition (2011), pp. 119-150, doi:10.1007/978-94-007-0114-4_9 by Denis Allemand, Éric Tambutté, Didier Zoccola, Sylvie Tambutté edited by Zvy Dubinsky, Noga Stambler posted by 2 people rayjl plaissue Abstract Copy My Attachments My Copy Abstract In spite of more than one century and half of studies, mechanisms of coral biomineralization, leading to coral growth and reef formation, still remain poorly known, although major global threats to coral reefs, such as ocean acidification, primarily affect this process. Coral skeletons are used as environmental archives but the vital processes that govern incorporation of trace elements and stable isotope are still unknown. Our knowledge on coral physiology is restricted to the organismal level due to the lack of appropriate ...
	✔ Registering histologic and MR images of prostate for image-based cancer detection. [Quick Edit] [CiTO] Academic radiology, Vol. 14, No. 11. (November 2007), pp. 1367-1381, doi:10.1016/j.acra.2007.07.018 by Yiqiang Zhan, Yangming Ou, Michael Feldman, John Tomaszeweski, Christos Davatzikos, Dinggang Shen posted by 2 people ben500 plaissue Abstract Copy My Attachments My Copy Abstract RATIONALE AND OBJECTIVES: Needle biopsy is currently the only way to confirm prostate cancer. To increase prostate cancer diagnostic rate, needles are expected to be deployed at suspicious cancer locations. High-contrast magnetic resonance (MR) imaging provides a powerful tool for detecting suspicious cancerous tissues. To do this, MR appearances of cancerous tissue should be characterized and learned from a sufficient number of prostate MR images with known cancer information. However, ground-truth cancer information is only available in histologic images. Therefore it ...
	✔ A syntaxin 1, Galpha(o), and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization. [Quick Edit] [CiTO] The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, No. 16. (21 April 2004), pp. 4070-4081, doi:10.1523/jneurosci.0346-04.2004 by Qi Li, Anthony Lau, Terence J. Morris, Lin Guo, Christopher B. Fordyce, Elise F. Stanley posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract Presynaptic Ca(V)2.2 (N-type) calcium channels are subject to modulation by interaction with syntaxin 1 and by a syntaxin 1-sensitive Galpha(O) G-protein pathway. We used biochemical analysis of neuronal tissue lysates and a new quantitative test of colocalization by intensity correlation analysis at the giant calyx-type presynaptic terminal of the chick ciliary ganglion to explore the association of Ca(V)2.2 with syntaxin 1 and Galpha(O). Ca(V)2.2 could ...
	✔ Bell Labs and the ruby laser [Quick Edit] [CiTO] Physics Today, Vol. 63, No. 1. (2010), pp. 40-45 by Donald F. Nelson, Robert J. Collins, Wolfgang Kaiser posted by 4 people plaissue rodney moernerlab samjlord Abstract Copy My Attachments My Copy Abstract With the 50th anniversary of the first laser approaching, its early history once again takes center stage. Several books in recent years have related that history from several points of view. Among the authors are Theodore Maiman, who first observed laser action in crystalline pink ruby, and Charles Townes, who invented the original ammonia maser and shared the 1964 Nobel Prize in Physics with Nicolay Basov and Aleksandr Prokhorov for the “maser–laser principle.” But apart from our publication of scientific papers ...
	✔ Yeast and fungal morphogenesis from an evolutionary perspective. [Quick Edit] [CiTO] Seminars in cell & developmental biology, Vol. 19, No. 3. (June 2008), pp. 224-233, doi:10.1016/j.semcdb.2008.01.003 by Roland Wedlich-Soldner, Rong Li posted by 2 people plaissue Keksle Abstract Copy My Attachments My Copy Abstract Cellular morphogenesis is a complex process and molecular studies in the last few decades have amassed a large amount of information that is difficult to grasp in any completeness. Fungal systems, in particular the budding and fission yeasts, have been important players in unravelling the basic structural and regulatory elements involved in a wide array of cellular processes. In this article, we address the design principles underlying the various processes of yeast and fungal morphogenesis. We attempt to explain the apparent ...
	✔ Mechanisms of polarized growth and organelle segregation in yeast. [Quick Edit] [CiTO] Annual review of cell and developmental biology, Vol. 20 (2004), pp. 559-591, doi:10.1146/annurev.cellbio.20.010403.103108 by David Pruyne, Aster Legesse-Miller, Lina Gao, Yuqing Dong, Anthony Bretscher posted by 2 people plaissue Keksle Abstract Copy My Attachments My Copy Abstract Cell polarity, as reflected by polarized growth and organelle segregation during cell division in yeast, appears to follow a simple hierarchy. On the basis of physical cues from previous cell cycles or stochastic processes, yeast cells select a site for bud emergence that also defines the axis of cell division. Once polarity is established, rho protein-based signal pathways set up a polarized cytoskeleton by activating localized formins to nucleate and assemble polarized actin cables. These serve as tracks for the transport ...
	✔ Computer and Robot Vision [Quick Edit] [CiTO] (1992) by Robert M. Haralick, Linda G. Shapiro posted by 3 people plaissue mthomure stsaft Abstract Copy My Attachments My Copy Abstract From the Publisher: This two-volume set is an authoritative, comprehensive, modern work on computer vision that covers all of the different areas of vision with a balanced and unified approach. The discussion in "Volume I" focuses on image in, and image out or feature set out. "Volume II" covers the higher level techniques of illumination, perspective projection, analytical photogrammetry, motion, image matching, consistent labeling, model matching, and knowledge-based vision systems. ...
	✔ Image Analysis and Mathematical Morphology, Vol. 2: Theoretical Advances [Quick Edit] [CiTO] (11 February 1988) posted by 2 people plaissue foleyfresh Copy My Attachments My Copy
	✔ A Short Story of Aequorin [Quick Edit] [CiTO] Biol Bull, Vol. 189, No. 1. (1 August 1995), pp. 1-5 by O. Shimomura posted by 3 people plaissue moernerlab samjlord Copy My Attachments My Copy
	✔ The creation of a brain atlas for image guided neurosurgery using serial histological data. [Quick Edit] [CiTO] NeuroImage, Vol. 30, No. 2. (1 April 2006), pp. 359-376, doi:10.1016/j.neuroimage.2005.09.041 by Mallar M. Chakravarty, Gilles Bertrand, Charles P. Hodge, Abbas F. Sadikot, D. Louis Collins posted by 2 people plaissue nguizard Abstract Copy My Attachments My Copy Abstract Digital and print brain atlases have been used with success to help in the planning of neurosurgical interventions. In this paper, a technique presented for the creation of a brain atlas of the basal ganglia and the thalamus derived from serial histological data. Photographs of coronal histological sections were digitized and anatomical structures were manually segmented. A slice-to-slice nonlinear registration technique was used to correct for spatial distortions introduced into the histological data set at the time of acquisition. Since the ...
	✔ The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. [Quick Edit] [CiTO] Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 10. (9 May 2000), pp. 5273-5278, doi:10.1073/pnas.080585897 by P. A. Takizawa, R. D. Vale posted by 2 people plaissue jonathanjacobs Abstract Copy My Attachments My Copy Abstract In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of the five She proteins, She2p, She3p, and Myo4p, colocalize with Ash1 mRNA in vivo and coimmunoprecipitate with Ash1 mRNA from cell extracts. We also find that She3p binds to Myo4p in the absence of RNA and She2p is required for binding ...
	✔ Reef corals bleach to resist stress [Quick Edit] [CiTO] Marine Pollution Bulletin, Vol. 58, No. 2. (08 February 2009), pp. 206-212, doi:10.1016/j.marpolbul.2008.10.002 by David O. Obura posted by 3 people udhiekohernawan danlordan plaissue Abstract Copy My Attachments My Copy Abstract A rationale is presented here for a primary role of bleaching in regulation of the coral–zooxanthellae symbiosis under conditions of stress. Corals and zooxanthellae have fundamentally different metabolic rates, requiring active homeostasis to limit zooxanthellae production and manage translocated products to maintain the symbiosis. The control processes for homeostasis are compromised by environmental stress, resulting in metabolic imbalance between the symbionts. For the coral–zooxanthella symbiosis the most direct way to minimize metabolic imbalance under stress is to reduce photosynthetic production by ...
	✔ Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons [Quick Edit] [CiTO] Nature Cell Biology, Vol. 13, No. 1. (12 January 2011), pp. 40-48, doi:10.1038/ncb2132 by Wolfgang Wagner, Stephan D. Brenowitz, John A. Hammer posted by 2 people plaissue antonkratz Abstract Copy My Attachments My Copy Abstract The role of actin-based motors and the mode of endoplasmic reticulum (ER) transport into spines had remained unclear. Myosin-Va is now shown to act as a point-to-point ER transporter into dendritic spines. ...
	✔ Mutual information for automated unwarping of rat brain autoradiographs. [Quick Edit] [CiTO] NeuroImage, Vol. 5, No. 1. (January 1997), pp. 31-40, doi:10.1006/nimg.1996.0251 by B. Kim, J. L. Boes, K. A. Frey, C. R. Meyer posted by 2 people plaissue jclau Abstract Copy My Attachments My Copy Abstract An automated multimodal warping based on mutual information metric (MI) as a mapping cost function is demonstrated. Mutual information (I) is calculated from a two-dimensional (2D) gray scale histogram of an image pair, and MI (= -I) provides a matching cost function which can be effective in registration of two- or three-dimensional data sets independent of modality. Most histological image data, though information rich and high resolution, present nonlinear deformations due to the specimen sectioning and need reconstitution into deformation-corrected volumes ...
	✔ Specific organic matrix characteristics in skeletons of <i>Corallium</i> species [Quick Edit] [CiTO] Marine Biology (23 August 2011), pp. 1-10, doi:10.1007/s00227-011-1775-7 by J. Debreuil, S. Tambutté, D. Zoccola, et al.N. Segonds, N. Techer, C. Marschal, D. Allemand, S. Kosuge, Tambutté posted by 2 people plaissue ericcsm Abstract Copy My Attachments My Copy Abstract Corallium species have ecological, cultural and commercial importance and thus require tools to assist with their identification for both management and trade. The organic matrix (OM) of the skeletons of four Corallium species ( C. rubrum , C. konojoi , C. secundum and C. elatius ) was examined to provide insight into the biomineralization process and to develop a new tool of identification. The pattern of OM and the set of soluble organic matrix proteins (SOM) in the skeletons were examined ...
	✔ The fungus Armillaria bulbosa is among the largest and oldest living organisms [Quick Edit] [CiTO] Nature, Vol. 356, No. 6368. (02 April 1992), pp. 428-431, doi:10.1038/356428a0 by Myron L. Smith, Johann N. Bruhn, James B. Anderson posted by 4 people kvalyi Mycology stajich plaissue Copy My Attachments My Copy
	✔ Resonance energy transfer in cells: a new look at fixation effect and receptor aggregation on cell membrane. [Quick Edit] [CiTO] Biophysical journal, Vol. 95, No. 3. (21 August 2008), pp. 1349-1359, doi:10.1529/biophysj.107.124313 by Max Anikovsky, Lianne Dale, Stephen Ferguson, Nils Petersen posted by 2 people plaissue paulschlesinger Abstract Copy My Attachments My Copy Abstract Fluorescence resonance energy transfer (FRET) measurements offer a reliable and noninvasive approach to studying protein and lipid colocalization in cells. We have considered systems in which FRET occurs as intramolecular and/or intermolecular process. The proposed dynamic FRET model shows that in the case of intermolecular process the degree of aggregation only slightly affects the energy transfer efficiency. The theory was tested on a set of donor-acceptor pairs in which energy transfer occurs intramolecularly, intermolecularly, or both. The obtained experimental results are ...
	✔ Gaussian approximations of fluorescence microscope point-spread function models [Quick Edit] [CiTO] Appl. Opt., Vol. 46, No. 10. (1 April 2007), pp. 1819-1829, doi:10.1364/ao.46.001819 by Bo Zhang, Josiane Zerubia, Jean-Christophe Olivo-Marin posted by 3 people plaissue willie_gt NE_baer Abstract Copy My Attachments My Copy Abstract We comprehensively study the least-squares Gaussian approximations of the diffraction-limited 2D-3D paraxial-nonparaxial point-spread functions (PSFs) of the wide field fluorescence microscope (WFFM), the laser scanning confocal microscope (LSCM), and the disk scanning confocal microscope (DSCM). The PSFs are expressed using the Debye integral. Under an L∞ constraint imposing peak matching, optimal and near-optimal Gaussian parameters are derived for the PSFs. With an L1 constraint imposing energy conservation, an optimal Gaussian parameter is derived for the 2D paraxial WFFM PSF. We found ...
	✔ Central roles of small GTPases in the development of cell polarity in yeast and beyond. [Quick Edit] [CiTO] Microbiology and molecular biology reviews : MMBR, Vol. 71, No. 1. (March 2007), pp. 48-96, doi:10.1128/mmbr.00028-06 by Hay-Oak O. Park, Erfei Bi posted by 2 people plaissue Keksle Abstract Copy My Attachments My Copy Abstract Summary: The establishment of cell polarity is critical for the development of many organisms and for the function of many cell types. A large number of studies of diverse organisms from yeast to humans indicate that the conserved, small-molecular-weight GTPases function as key signaling proteins involved in cell polarization. The budding yeast Saccharomyces cerevisiae is a particularly attractive model because it displays pronounced cell polarity in response to intracellular and extracellular cues. Cells of S. cerevisiae undergo polarized growth during various ...
	✔ An Algorithm for Least-Squares Estimation of Nonlinear Parameters [Quick Edit] [CiTO] Journal of the Society for Industrial and Applied Mathematics, Vol. 11, No. 2. (1963), pp. 431-441, doi:10.2307/2098941 by Donald W. Marquardt posted by 7 people plaissue xyz123 bruno dcombest Aolty MdSalam eyliu Copy My Attachments My Copy
	✔ A Model for Multiparametric MRI Tissue Characterization in Experimental Cerebral Ischemia With Histological Validation in Rat : Part 1 [Quick Edit] [CiTO] Stroke, Vol. 32, No. 4. (1 April 2001), pp. 943-949 by Michael A. Jacobs, Zheng G. Zhang, Robert A. Knight, et al.Hamid Soltanian-Zadeh, Anton V. Goussev, Donald J. Peck, Michael Chopp posted by 2 people lrittner plaissue Abstract Copy My Attachments My Copy Abstract Background and Purpose--After stroke, brain tissue undergoes time-dependent heterogeneous histopathological change. These tissue alterations have MRI characteristics that allow segmentation of ischemic from nonischemic tissue. Moreover, MRI segmentation generates different zones within the lesion that may reflect heterogeneity of tissue damage. Methods--A vector tissue signature model is presented that uses multiparametric MRI for segmentation and characterization of tissue. An objective (unsupervised) computer segmentation algorithm was incorporated into this model with the use of a modified version of the Iterative Self-Organizing ...
	✔ A scheme for automatically building three-dimensional morphometric anatomical atlases: application to a skull atlas. [Quick Edit] [CiTO] Medical image analysis, Vol. 2, No. 1. (March 1998), pp. 37-60 by G. Subsol, J. P. Thirion, N. Ayache posted by 2 people jclau plaissue Abstract Copy My Attachments My Copy Abstract We present a general scheme for automatically building a morphometric anatomical atlas. We detail each stage of the method, including the non-rigid registration algorithm, three-dimensional line averaging and statistical processes. We apply the method to obtain a quantitative atlas of skull crest lines. Finally, we use the resulting atlas to study a craniofacial disease; we show how we can obtain qualitative and quantitative results by ...
	✔ Excited state reactions in fluorescent proteins [Quick Edit] [CiTO] Chem. Soc. Rev., Vol. 38, No. 10. (2009), pp. 2922-2934, doi:10.1039/b820168b by Stephen R. Meech posted by 5 people danmaftei joachimg plaissue moernerlab samjlord Abstract Copy My Attachments My Copy Abstract The green fluorescent protein is a key technology in bioimaging. In this critical review, we consider how its various applications can be tailored from knowledge of the excited state chemistry. The photophysics of the basic chromophore in solution are described in detail, and the dominant radiationless decay mechanism is characterised. The quite different photophysics of wild type GFP are described next. The unique excited state proton transfer reaction observed can be used to model proton transfer processes in proteins. Examples where ...
	✔ Labels and Probes for Live Cell Imaging: Overview and Selection Guide [Quick Edit] [CiTO] In Live Cell Imaging, Vol. 591 (2010), pp. 17-45, doi:10.1007/978-1-60761-404-3_2 by Hilderbrand edited by Dmitri .. Papkovsky, posted by 4 people fergus plaissue moernerlab samjlord Abstract Copy My Attachments My Copy Abstract Fluorescence imaging is an important tool for molecular biology research. There is a wide array of fluorescent labels and activatable probes available for investigation of biochemical processes at a molecular level in living cells. Given the large number of potential imaging agents and numerous variables that can impact the utility of these fluorescent materials for imaging, selection of the appropriate probes can be a difficult task. In this report an overview of fluorescent imaging agents and details on their optical and ...
	✔ Apical sterol-rich membranes are essential for localizing cell end markers that determine growth directionality in the filamentous fungus Aspergillus nidulans. [Quick Edit] [CiTO] Molecular biology of the cell, Vol. 19, No. 1. (1 January 2008), pp. 339-351, doi:10.1091/mbc.e07-06-0523 by Norio Takeshita, Yuhei Higashitsuji, Sven Konzack, Reinhard Fischer posted by 1 person plaissue Abstract Copy My Attachments My Copy Abstract In filamentous fungi, hyphal extension depends on the continuous delivery of vesicles to the growing tip. Here, we describe the identification of two cell end marker proteins, TeaA and TeaR, in Aspergillus nidulans, corresponding to Tea1 and Mod5 in Schizosaccharomyces pombe. Deletion of teaA or teaR caused zig-zag-growing and meandering hyphae, respectively. The Kelch-repeat protein TeaA, the putatively prenylated TeaR protein, and the formin SepA were highly concentrated in the Spitzenkörper, a vesicle transit station at the tip, and localized along ...
	✔ Class III chitin synthase ChsB of Aspergillus nidulans localizes at the sites of polarized cell wall synthesis and is required for conidial development. [Quick Edit] [CiTO] Eukaryotic cell, Vol. 8, No. 7. (1 July 2009), pp. 945-956, doi:10.1128/ec.00326-08 by Kazuharu Fukuda, Kazunari Yamada, Ken Deoka, Shuichi Yamashita, Akinori Ohta, Hiroyuki Horiuchi posted by 3 people Mycology stajich plaissue Abstract Copy My Attachments My Copy Abstract Class III chitin synthases play important roles in tip growth and conidiation in many filamentous fungi. However, little is known about their functions in those processes. To address these issues, we characterized the deletion mutant of a class III chitin synthase-encoding gene of Aspergillus nidulans, chsB, and investigated ChsB localization in the hyphae and conidiophores. Multilayered cell walls and intrahyphal hyphae were observed in the hyphae of the chsB deletion mutant, and wavy septa were also occasionally observed. ChsB tagged with ...

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