CiteULike: Author Cazenave

Characterization of CD1e, a Third Type of CD1 Molecule Expressed in Dendritic Cells

Catherine Angenieux — 2008-05-27T03:57:03-00:00

J. Biol. Chem., Vol. 275, No. 48. (22 November 2000), pp. 37757-37764.

Dendritic cells express several alternatively spliced CD1e mRNAs. These molecules encode proteins characterized by the presence of either one, two, or three [alpha] domains and either a 51- or 63-amino acid cytoplasmic domain. Moreover, mRNAs encoding isoforms lacking the transmembrane domain are observed. Several of these CD1e isoforms were expressed in transfected cells, and two of them, with three [alpha] domains, displayed a particular processing pathway. These latter isoforms slowly leave the endoplasmic reticulum due to the presence of atypical dilysine motifs in the cytoplasmic tail. These molecules are associated with the [beta]2-microglobulin and accumulate in late Golgi and late endosomal compartments. In the latter compartments, they are cleaved into soluble forms that appear to be stable. In dendritic cells, these isoforms are mainly located in the Golgi apparatus, and upon maturation they are redistributed to late endosomal compartments. This work demonstrates the existence of CD1e molecules. As compared with other CD1 molecules, CD1e displays fundamentally different properties and therefore may represent a third type of CD1 molecules. 10.1074/jbc.M007082200

Conservation et valorisation du patrimoine des organismes de recherche

Frédoc 2006 — 2008-04-25T07:16:22-00:00

(07 February 2008)

Computer Go: An AI oriented survey

Bruno Bouzy — 2008-02-19T11:33:03-00:00

Artificial Intelligence, Vol. 132, No. 1. (2001), pp. 39-103.

Since the beginning of AI, mind games have been studied as relevant application fields. Nowadays, some programs are better than human players in most classical games. Their results highlight the efficiency of AI methods that are now quite standard. Such methods are very useful to Go programs, but they do not enable a strong Go program to be built. The problems related to Computer Go require new AI problem solving methods. Given the great number of problems and the diversity of possible...

The Bw Cells, a Novel B Cell Population Conserved in the Whole Genus Mus

Aude Thiriot — 2007-11-13T16:38:38-00:00

J Immunol, Vol. 179, No. 10. (15 November 2007), pp. 6568-6578.

In common laboratory mouse strains, which are derived from the crossing between three subspecies, peritoneal B cells are enriched in B-1a cells characterized by the CD5+Mac-1+B220lowIgMhighIgDlowCD43+CD9+ phenotype. Intriguingly in other vertebrates, CD5+Mac-1+ cells have never been found in a specific anatomic site. To ascertain the peculiarity of the CD5+ peritoneal B cells in laboratory mice, we analyzed the peritoneal B cell subsets in 9 inbred and 39 outbred wild-derived mouse strains belonging to 13 different species/subspecies. We found that most of these strains do not have the CD5+ B-1a cell population. However, all of these strains including classical laboratory mouse strains, have variable proportions of a novel B cell population: Bw, which is characterized by a unique phenotype (CD5Mac-1+B220highIgMhighIgDhighCD43CD9) and is not restricted to the peritoneal cavity. Bw cells are also distinct from both B-1 and B-2 cells from a functional point of view both by proliferative responses, cytokine secretion and Ab synthesis. Moreover, transfer experiments show that bone marrow and fetal liver cells from wild mice can give rise to Bw cells in alymphoid mice. The conservation of this B cell population, but not of the CD5+ B-1a, during evolution of the genus Mus, its readiness to respond to TLR ligands and to produce high concentration of autoantibodies suggest that Bw cells play a key role in innate immunity.

Rab11A controls the biogenesis of Birbeck granules by regulating Langerin recycling and stability.

S Uzan-Gafsou — 2007-10-22T08:50:23-00:00

Mol Biol Cell, Vol. 18, No. 8. (August 2007), pp. 3169-3179.

The extent to which Rab GTPases, Rab-interacting proteins, and cargo molecules cooperate in the dynamic organization of membrane architecture remains to be clarified. Langerin, a recycling protein accumulating in the Rab11-positive compartments of Langerhans cells, induces the formation of Birbeck granules (BGs), which are membrane subdomains of the endosomal recycling network. We investigated the role of Rab11A and two members of the Rab11 family of interacting proteins, Rip11 and RCP, in Langerin traffic and the biogenesis of BGs. The overexpression of a dominant-negative Rab11A mutant or Rab11A depletion strongly influenced Langerin traffic and stability and the formation of BGs, whereas modulation of other Rab proteins involved in dynamic regulation of the endocytic-recycling pathway had no effect. Impairment of Rab11A function led to a missorting of Langerin to lysosomal compartments, but inhibition of Langerin degradation by chloroquine did not restore the formation of BGs. Loss of RCP, but not of Rip11, also had a modest, but reproducible effect on Langerin stability and BG biogenesis, pointing to a role for Rab11A-RCP complexes in these events. Our results show that Rab11A and Langerin are required for BG biogenesis, and they illustrate the role played by a Rab GTPase in the formation of a specialized subcompartment within the endocytic-recycling system.

Floodplain water storage in the Negro River basin estimated from microwave remote sensing of inundation area and water levels

Frederic Frappart — 2007-10-08T13:46:20-00:00

Remote Sensing of Environment, Vol. 99, No. 4. (15 December 2005), pp. 387-399.

The objective of this study is to determine spatio-temporal variations of water volume over inundated areas located in large river basins using combined observations from the Synthetic Aperture Radar (SAR) onboard the Japanese Earth Resources Satellite (JERS-1), the Topex/Poseidon (T/P) altimetry satellite, and in-situ hydrographic stations. Ultimately, the goal is to quantify the role of floodplains for partitioning water and sediment fluxes over the great fluvial basins of the world. SAR images are used to identify the type of surface (open water, inundated areas, forest) and, hence, the areas covered with water. Both radar altimetry data and in-situ hydrographic measurements yield water level time series. The basin of the Negro River, the tributary which carries the largest discharge to the Amazon River, was selected as a test site. By combining area estimates derived from radar images classification with changes in water level, variations of water volume (focusing on a seasonal cycle) have been obtained. The absence of relationship between water volume and inundated area, reflecting the diverse and widely dispersed floodplains of the basin, is one of the main result of this study.

Computer Go: An AI oriented survey

Bruno Bouzy — 2006-07-31T17:41:59-00:00

Artificial Intelligence, Vol. 132, No. 1. (October 2001), pp. 39-103.

Since the beginning of AI, mind games have been studied as relevant application fields. Nowadays, some programs are better than human players in most classical games. Their results highlight the efficiency of AI methods that are now quite standard. Such methods are very useful to Go programs, but they do not enable a strong Go program to be built. The problems related to Computer Go require new AI problem solving methods. Given the great number of problems and the diversity of possible solutions, Computer Go is an attractive research domain for AI. Prospective methods of programming the game of Go will probably be of interest in other domains as well. The goal of this paper is to present Computer Go by showing the links between existing studies on Computer Go and different AI related domains: evaluation function, heuristic search, machine learning, automatic knowledge generation, mathematical morphology and cognitive science. In addition, this paper describes both the practical aspects of Go programming, such as program optimization, and various theoretical aspects such as combinatorial game theory, mathematical morphology, and Monte Carlo methods.